| 出品公司: | Takara |
|---|---|
| 载体名称: | pEF1α-Tet3G |
| 载体又名: |
pEF1α-Tet3G载体，pEF1α-Tet3G质粒，pEF1α-Tet3G载体质粒 pEF1 alpha-Tet3G载体，pEF1 alpha-Tet3G质粒，pEF1alpha-Tet3G载体质粒 |
| 英文名称: |
pEF1α-Tet3G, pEF1α-Tet3G Vector, pEF1α-Tet3G Plasmid pEF1alpha-Tet3G, pEF1 alpha-Tet3G Vector, pEF1 alpha-Tet3G Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | EF1α |
| 载体大小: | 7.9 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 新霉素（Neomycin） |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | 631167 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pEF1α-Tet3G载体 pEF1alpha-Tet3G质粒基本信息
pEF1α-Tet3G质粒图谱 pEF1alpha-Tet3G载体图谱和pEF1α-Tet3G载体序列 pEF1alpha-Tet3G质粒序列多克隆位点信息
pEF1α-Tet3G质粒 pEF1alpha-Tet3G载体简介
pEF1α-Tet3G质粒序列 pEF1alpha-Tet3G载体序列
LOCUS pEF1α-Tet3G 7859 bp DNA circular SYN 11-JUN-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7859)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Jun 11, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7859
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 206..1387
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
/note="color: #ffffff; direction: RIGHT"
intron 436..1378
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
/note="color: #------"
CDS 1495..2241
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/note="color: #993366"
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
polyA_signal 2251..2385
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(3105..3693)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(3864..4724)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 3864 .. 4655 / #ccffcc
2: 4656 .. 4724 / #ccffcc / signal sequence
Cleavage site after base 4655"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4725..4829)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
polyA_signal 4942..5076
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
CDS complement(5501..5521)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/note="color: #993366"
/translation="PKKKRKV"
intron 5651..5716
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
/note="color: #------"
CDS complement(6136..6930)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/note="color: #ccffcc"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(7291..7620)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
/note="color: #ffffff; direction: LEFT"
rep_origin 7305..7440
/label=SV40 ori
/note="SV40 origin of replication"
/note="color: #ffff00"
ORIGIN
1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta cagagtaatt
61 catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact
121 aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac
181 tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
241 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
301 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
361 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
421 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
481 tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga
541 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
601 ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg
661 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
721 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
781 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
841 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
901 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
961 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
1021 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
1081 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
1141 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
1201 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
1261 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
1321 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
1381 gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
1441 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccatgtct
1501 agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg agtcggtatc
1561 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
1621 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
1681 catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
1741 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
1801 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
1861 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
1921 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
1981 accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc
2041 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
2101 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
2161 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
2221 cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata cattgatgag
2281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
2341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
2401 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
2461 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca
2521 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc
2581 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc
2641 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg
2701 aagtatccag ctcgaccaag cttggcgaga ttttcaggag ctaaggaagc taaaatggag
2761 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
2821 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct gcattaatga
2881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
2941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
3001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
3061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
3121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
3181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
3241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
3301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
3361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
3421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
3481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
3541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
3601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
4021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
4081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
4141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
4201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
4261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
4321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
4381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
4441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
4501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
4561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
4621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
4861 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
4921 cgtcttcact cgaggtcgag ggatccagac atgataagat acattgatga gtttggacaa
4981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
5041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
5101 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
5161 tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa
5221 cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag
5281 acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc
5341 tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt
5401 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
5461 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
5521 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
5581 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
5641 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
5701 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
5761 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
5821 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
5881 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc
5941 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
6001 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
6061 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
6121 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
6181 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
6241 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
6301 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
6361 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
6421 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
6481 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
6541 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
6601 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
6661 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
6721 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
6781 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
6841 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
6901 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
6961 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
7021 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
7081 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
7141 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
7201 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
7261 tgagtgcttg cggcagcgtg aagctagctt tttgcaaaag cctaggcctc caaaaaagcc
7321 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
7381 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
7441 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
7501 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
7561 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
7621 attccacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
7681 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
7741 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
7801 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcacct
//
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