引物名称: | Xpress Forward、Xpress-F |
---|---|
引物又名: | Xpress Forward引物、Xpress-F引物、Xpress Forward测序引物、Xpress-F测序引物、Xpress Forward通用引物、Xpress-F通用引 物、Xpress Forward通用测序引物、Xpress-F通用测序引物、Xpress Forward Primer、 Xpress-F Primer、Xpress Forward Sequencing Primer、Xpress-F Sequencing Primer、 |
引物序列: | TATGGCTAGCATGACTGGT (Invitrogen) |
引物说明: | Xpress epitope, forward primer |
引物类型: | 测序引物,通用引物,通用测序引物 |
级别: | HPLC |
用途: | 测序, PCR |
产品目录号: | P1132 |
产品规格: | 2OD、 10OD |
保存条件: | 密封,2-25℃保存。 |
Commonly Used Primers
CMV Forward |
CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter, forward primer |
LKO.1 5' |
GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer |
LucNrev |
CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer |
M13 Reverse |
CAGGAAACAGCTATGAC In lacZ gene |
MSCV |
CCCTTGAACCTCCTCGTTCGACC (BD Biosciences) Murine stem cell virus, forward primer |
pBABE 5' |
CTTTATCCAGCCCTCAC (Weinberg Lab) Psi packaging signal, 5' of MCS in pBABE vectors, forward primer |
pGEX 5' |
GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer |
SP6 |
ATTTAGGTGACACTATAG SP6 promoter, forward primer |
T3 |
GCAATTAACCCTCACTAAAGG T3 promoter, forward primer |
T7 |
TAATACGACTCACTATAGGG T7 promoter, forward primer |
Full Primer List
3'AOX1 |
GCAAATGGCATTCTGACATCC (Invitrogen) For Pichia vectors with AOX1 terminator, reverse primer |
5'AOX1 |
GACTGGTTCCAATTGACAAGC (Invitrogen) For Pichia vectors with AOX1 promoter, forward primer |
35S promoter |
CTATCCTTCGCAAGACCCTTC CaMV 35S promoter, forward primer |
AC5 |
ACACAAAGCCGCTCCATCAG (Invitrogen) Drosophila Actin 5C promoter, forward primer |
Alpha-factor |
TACTATTGCCAGCATTGCTGC (Invitrogen) Alpha factor signal sequence, forward primer |
Amp-R |
ATAATACCGCGCCACATAGC 5' end of ampicillin resistance gene, reverse primer |
AUG1 Forward |
CAATTTACATCTTTATTTATTAACG (Invitrogen) For Pichia vectors with AUG1 promoter, forward primer |
AUG1 Reverse |
GAAGAGAAAAACATTAGTTGGC (Invitrogen) For Pichia vectors with AUG1 promoter, reverse primer |
BGH Reverse |
TAGAAGGCACAGTCGAGG (Invitrogen) Bovine growth hormone terminator, reverse primer |
Bglob-intron-F |
CTGGTCATCATCCTGCCTTT Rabbit beta-globin intron, forward primer |
Bglob-intron-R |
TTTGCCCCCTCCATATAACA Rabbit beta-globin intron, reverse primer |
Bglob-pA-R |
TTTTGGCAGAGGGAAAAAGA Rabbit beta-globin polyA region, reverse primer |
CAT-R |
GCAACTGACTGAAATGCCTC 5' end of chloramphenicol resistance gene, reverse primer |
CMV Forward |
CGCAAATGGGCGGTAGGCGTG (Invitrogen) Human CMV immediate early promoter, forward primer |
CRE-R |
GCAAACGGACAGAAGCATTT 5' end of Cre recombinase, reverse primer |
CYC1 |
GCGTGAATGTAAGCGTGAC (Invitrogen) CYC1 transcription termination signal, reverse primer |
DsRed1-C |
AGCTGGACATCACCTCCCACAACG (BD Biosciences) 3' end of DsRed1, forward primer |
DsRed1-N |
GTACTGGAACTGGGGGGACAG (BD Biosciences) 5' end of DsRed1, reverse primer |
EBV Reverse |
GTGGTTTGTCCAAACTCATC (Invitrogen) SV40 polyA terminator, reverse primer |
Ecdysone Forward |
CTCTGAATACTTTCAACAAGTTAC (Invitrogen) Drosophila heat shock promoter, forward primer |
EF-1a Forward |
TCAAGCCTCAGACAGTGGTTC (Invitrogen) Human elongation factor-1a promoter, forward primer |
EGFP-C |
CATGGTCCTGCTGGAGTTCGTG (BD Biosciences) 3' end of EGFP, forward primer |
EGFP-N |
CGTCGCCGTCCAGCTCGACCAG (BD Biosciences) 5' end of EGFP, reverse primer |
EXFP-R |
GTCTTGTAGTTGCCGTCGTC (Golenbock lab) For distinguishing EGFP vs ECFP vs EYFP, reverse primer |
F1ori-F |
GTGGACTCTTGTTCCAAACTGG F1 origin, forward primer |
GAL1 |
AATATACCTCTATACTTTAACGTC (Invitrogen) S. cerevisiae GAL1 promoter, forward primer |
Gal10pro-F |
GGTGGTAATGCCATGTAATATG (Stratagene) S. cerevisiae GAL10 promoter, forward primer |
Gal4 N-term |
GAGTAGTAACAAAGGTCAA 3' end of Gal4 DNA binding domain, forward primer |
Gal4-AD |
AATACCACTACAATGGAT (BD Biosciences) 3' end of Gal4 activation domain, forward primer |
GFP-F |
GGTCCTTCTTGAGTTTGTAAC 3' end of GFP, forward primer |
GFP-R |
CCATCTAATTCAACAAGAATTGGGACAAC (Ahmad lab) 5' end of GFP, reverse primer |
GPDpro-F |
CGGTAGGTATTGATTGTAATTCTG S. cerevisiae GPD promoter, forward primer |
GW-3' |
GCATGATGACCACCGATATG 3' end of Gateway cassette, forward primer |
GW-5' |
AATCTCGCCGGATCCTAACT 5' end of Gateway cassette, reverse primer |
H1 |
TCGCTATGTGTTCTGGGAAA Human H1 promoter, forward primer |
HA-F |
TACCCATACGACGTCCCAGA HA tag, forward primer |
HA-R |
TCTGGGACGTCGTATGGGTA HA tag, reverse primer |
HAT |
GAGGAGCACGCTCATGCCCAC (BD Biosciences) Histidine affinity tag, forward primer |
hGH-PA-R |
CCAGCTTGGTTCCCAATAGA Human growth hormone terminator, reverse primer |
hrGFP-R |
TCCCCGAGTACCACTTCATC hrGFP (humanized Renilla GFP), forward primer |
hUBCpro-F |
TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter, forward primer |
IRES-F |
TGGCTCTCCTCAAGCGTATT 3' end of IRES, forward primer |
IRES-R |
CCTCACATTGCCAAAAGACG 5' end of IRES, reverse primer |
L4440 |
AGCGAGTCAGTGAGCGAG (Caldwell lab) 5' of MCS in L4440 vector, forward primer |
LacI-R |
GGCATACTCTGCGACATCGT 5' end of LacI, reverse primer |
LacZ-R |
GACAGTATCGGCCTCAGGAA 5' end of LacZ, reverse primer |
LexA |
CGTCAGCAGAGCTTCACCATTG (Caldwell lab) 3' end of LexA DNA binding domain, forward primer |
LKO.1 5' |
GACTATCATATGCTTACCGT (Weinberg Lab) Human U6 promoter, forward primer |
LNCX |
AGCTCGTTTAGTGAACCGTCAGATC (BD Biosciences) Human CMV promoter, forward primer |
Luc-F |
AGTCAAGTAACAACCGCGA 3' end of luciferase, forward primer |
LucNrev |
CCTTATGCAGTTGCTCTCC 5' end of luciferase, reverse primer |
M13 (-21) Forward |
TGTAAAACGACGGCCAGT In lacZ gene |
M13 (-40) |
GTTTTCCCAGTCACGAC In lacZ gene |
M13 Reverse |
CAGGAAACAGCTATGAC In lacZ gene |
M13/pUC Forward |
CCCAGTCACGACGTTGTAAAACG (Invitrogen) In lacZ gene |
M13/pUC Reverse |
AGCGGATAACAATTTCACACAGG (Invitrogen) In lacZ gene |
MBP-F |
GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) 3' end of maltose binding protein, forward primer |
mCherry-F |
CCCCGTAATGCAGAAGAAGA 3' end of mCherry, forward primer |
mCherry-R |
TTGGTCACCTTCAGCTTGG 5' end of mCherry, reverse primer |
MT Forward |
CATCTCAGTGCAACTAAA (Invitrogen) Drosophila metallothionein promoter, forward primer |
MMLV-F |
ATCAGTTCGCTTCTCGCTTC Moloney murine leukemia virus LTR (MoMuLV), forward primer |
mPGK-F |
CATTCTGCACGCTTCAAAAG Mouse PGK promoter, forward primer |
MSCV |
CCCTTGAACCTCCTCGTTCGACC (BD Biosciences) Murine stem cell virus, forward primer |
MSCV-rev |
CAGCGGGGCTGCTAAAGCGCATGC Murine stem cell virus, reverse primer |
MT1-F |
GCTGTCCTCTAAGCGTCACC Mouse metallothionein 1 promoter, forward primer |
mU6-F |
CAGCACAAAAGGAAACTCACC Mouse U6 promoter, forward primer |
Myc |
GCATCAATGCAGAAGCTGATCTCA (BD Biosciences) Myc tag, forward primer |
Neo-F |
CGTTGGCTACCCGTGATATT 3' end of neomycin resistance gene, forward primer |
Neo-R |
GCCCAGTCATAGCCGAATAG 5' end of neomycin resistance gene, reverse primer |
NOS-F |
GCGTTCAAAAGTCGCCTAAG Nopaline synthase promoter, forward primer |
Nmt1-F |
GCAATGTGCAGCGAAACTAA S. pombe nmt1 promoter, forward primer |
OpIE2 Forward |
CGCAACGATCTGGTAAACAC (Invitrogen) OpIE2 promoter, forward primer |
pACYC-F |
TGAAGTCAGCCCCATACGAT p15A origin, forward primer |
pAd-CMV |
GCTAGAGATCTGGTACCGTC For cloning sites after SalI in pAd-CMV vector |
pBABE 3' |
ACCCTAACTGACACACATTCC (Weinberg Lab) SV40 enhancer, 3' of MCS in pBABE vectors, reverse primer |
pBABE 5' |
CTTTATCCAGCCCTCAC (Weinberg Lab) Psi packaging signal, 5' of MCS in pBABE vectors, forward primer |
pBAD Forward |
ATGCCATAGCATTTTTATCC (Invitrogen) For vectors with E. coli araBAD promoter, forward primer |
pBAD Reverse |
GATTTAATCTGTATCAGG (Invitrogen) For vectors with E. coli araBAD promoter, reverse primer |
pBluescriptKS |
TCGAGGTCGACGGTATC For pBluescript vector |
pBluescriptSK |
TCTAGAACTAGTGGATC For pBluescript vector |
pBMN 5' |
GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector |
pBR322ori-F |
GGGAAACGCCTGGTATCTTT pBRS322 origin, forward primer |
pBRforBam |
CTTGGAGCCACTATCGAC In pBR322 tet region, upstream of BamHI, forward primer |
pBRforEco |
AATAGGCGTATCACGAGGC In pBR322, upsteam of EcoRI site, forward primer |
pBRrevBam |
GGTGATGTCGGCGATATAGG In pBR322 tet region, downstream of BamHI, reverse primer |
pCAG-F |
GCAACGTGCTGGTTATTGTG Rabbit beta-globin intron, for pCAG plasmids, forward primer |
pCasper-F |
GGGTTTTATTAACTTACAT (Vosshall lab) 5' end of Drosophila mini-white gene, reverse primer |
pCasper-hs |
GCAACTACTGAAATCTGCCAAG Drosophila Hsp70 promoter, forward primer |
pcDL-F |
GTTGCCTTTACTTCTAGGCCT (Kinet lab) 5' of EcoRI site in pcDL vector, forward primer |
pENTR-F |
CTACAAACTCTTCCTGTTAGTTAG 5' of attL1 in pENTR vector, forward primer |
pENTR-R |
ATGGCTCATAACACCCCTTG 3' of attL2 in pENTR vector, reverse primer |
pGEX 3' |
CCGGGAGCTGCATGTGTCAGAGG 3' of MCS in pGEX vectors, reverse primer |
pGEX 5' |
GGGCTGGCAAGCCACGTTTGGTG 3' end of glutathione-S-transferase, forward primer |
pGP704-R |
AACAAGCCAGGGATGTAACG R6K gamma origin, 3' of MCS in pGP704 vector, reverse primer |
pHybLex Reverse |
GAGTCACTTTAAAATTTGTATACAC (Invitrogen) ADH terminator, reverse primer |
pLTet-F |
ACTGAGCACATCAGCAGGAC Lambda phage early leftward (pL) promoter, forward primer |
pLXSN 5' |
CCCTTGAACCTCCTCGTTCGACC (MSCV) Murine stem cell virus, same as MSCV, forward primer |
pMRB101-F |
AAGATGCAGGCAGCTGAGTT HCMV major immediate-early protein (IE), forward primer |
pMT2-F |
TTGCCTTTCTCTCCACAGGT 3' end of synthetic intron, forward primer |
pMX-S1811 |
GACGGCATCGCAGCTTGGATACAC (Yamanaka lab) MMLV sequence, 5' of MCS in pMXs vector, forward primer |
Polyhedrin forward |
AAATGATAACCATCTCGC (Invitrogen) Polyhedrin promoter, forward primer |
Polyhedrin reverse |
GTCCAAGTTTCCCTG (Invitrogen) For baculovirus vector with polyhedrin promoter, reverse primer |
pQE promoter |
CCCGAAAAGTGCCACCTG (Qiagen) 5' of MCS in pQE vectors, forward primer |
pREP Forward |
GCTCGATACAATAAACGCC (Invitrogen) Rous sarcoma virus (RSV) promoter, forward primer |
pRS-marker |
CGGCATCAGAGCAGATTGTA To sequence yeast selectable marker in pRS vectors |
Pry1 |
CTTAGCATGTCCGTGGGGTTTGAAT PZ P-element, reverse primer |
pTrcHis Forward |
GAGGTATATATTAATGTATCG (Invitrogen) 5' of MCS in pTrcHis vector, forward primer |
pTrcHis Reverse |
GATTTAATCTGTATCAGG (Invitrogen) 3' of MCS in pTrcHis vector, same as pBAD-R, reverse primer |
Puro-F |
GCAACCTCCCCTTCTACGAGC 3' end of puromycin resistance gene, forward primer |
pZIP |
TCCTTTCCAGCGAGGTTCTA Murine leukemia virus (MuLV), reverse primer |
RCAS-F |
ACATGGGTGGTGGTATAGCGCTTGCG (Orsulic lab) 3' of Rous sarcoma virus (RSV) env gene, forward primer |
Rluc-F |
CCAGGATTCTTTTCCAATGC 3' end of Renilla luciferase, forward primer |
RVprimer3 |
CTAGCAAAATAGGCTGTCCC (Promega) 5' of MCS in pGL3 vector, forward primer |
SFFV-F |
ATTGATTGACTGCCCACCTC Spleen focus forming virus 5' LTR, forward primer |
SP6 |
ATTTAGGTGACACTATAG SP6 promoter, forward primer |
SV40pA-R |
GAAATTTGTGATGCTATTGC SV40 polyA, reverse primer |
SV40pro-F |
TATTTATGCAGAGGCCGAGG SV40 promoter/origin, forward primer |
SV40-spliceR |
CACAAAGATCCGGACCAAAG SV40 splice sequence, reverse primer |
T3 |
GCAATTAACCCTCACTAAAGG T3 promoter, forward primer |
T7 |
TAATACGACTCACTATAGGG T7 promoter, forward primer |
T7 Terminal |
GCTAGTTATTGCTCAGCGG T7 terminator, reverse primer |
Tac promoter |
GAGCGGATAACAATTTCACACAGG (Waugh lab) Tac promoter, forward primer |
tdTomato-Fwd |
CTGTTCCTGTACGGCATGG 3' end of tdTomato, forward primer |
tdTomato-Rev |
TCTTTGATGACGGCCATGT 5' end of tdTomato, reverse primer |
Tet-R |
GGCGAGTTTACGGGTTGTTA 5' end of tetracycline resistance gene, reverse primer |
TK-pA-R |
TTGTCTCCTTCCGTGTTTCA Thymidine kinase polyA, reverse primer |
Tn7-end |
GGGGTGGAAATGGAGTTTTT Bacterial transposon Tn7 |
TRC-F |
CAAGGCTGTTAGAGAGATAATTGGA (Root lab) Human U6 promoter, forward primer |
Ubx-F |
AACTCGTACTTTGAACAGGC Drosophila Ultrabithorax gene, forward primer |
V5 Reverse |
ACCGAGGAGAGGGTTAGGGAT (Invitrogen) V5 epitope, reverse primer |
WPRE-R |
CATAGCGTAAAAGGAGCAACA 5' end of WPRE, reverse primer |
XBG-R |
GACTCCATTCGGGTGTTC Xenopus beta-globin 3'UTR, reverse primer |
XEF1a |
TTTCGCCCTAACTTCGTGAT Xenopus EF1 alpha enhancer/promoter, forward primer |
Xpress Forward |
TATGGCTAGCATGACTGGT (Invitrogen) Xpress epitope, forward primer |