解脲支原体肉汤

价格:

规格: 2x500ML 6x500ML

联系方式:I47-825O-882O

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Ureaplasma Broth产品基本信息

产品名称: 解脲支原体肉汤;脲原体肉汤
英文名称: Ureaplasma Broth
级别: for microbiology
品牌名称: ELITE-MEDIA(艾礼培养基)
产品目录号: M880-01、M880-02
产品规格: 2x500ML、6x500ML(完全培养基)
产品外观: 橘黄色溶液。
保存条件: 避光,2-8°C保存,至少可以保存3个月。
注意事项: 避免摄入、吸入、皮肤接触。
相关产品: --
艾礼培养基


产品描述:

解脲支原体肉汤(Ureaplasma Broth)推荐用于培养和分离解脲支原体(Ureaplasma urealyticum)和人型支原体(Mycoplasma hominis)。


原理:

解脲支原体(M.urealyticum)为脲原体属中唯一菌种,生长需要胆固醇和尿素。分解尿素为其代谢特征,产生氨态氮,使培养基pH上升,导致自身死亡。菌落微小,直径仅有15~25um,须在低倍显微镜下观察,故旧称T株(tinystrain)。菌落表面有粗糙颗粒,在合适条件下可转成典型的荷包蛋样菌落。

该培养基基础是牛心肉汤,补充以新鲜的叫酵母提取物和马血清。通常加入青霉素以抑制其它细菌生长。酵母提取物是肺炎支原体(Mycoplasma pneumoniae)生长所必需的。其它人型菌株没有该需求。苯酚红作为培养基中的酸碱指示剂。



配方与配制方法

酪蛋白胨 17 g
大豆蛋白胨 3.0 g
D-葡萄糖 2.5 g
磷酸氢二钾 2.5 g
氯化钠 5.0 g
硫酸锰 0.2 g
10%尿素溶液 5.0 mL
NEL-X Enrichment 5.0 mL
马血清 155 mL
苯酚红 16 mg
氨苄青霉素 50 mg
两性霉素B 4 mg
蒸馏水 835 mL
pH 6.0-6.6



实验方法

It is advisable to inoculate all specimens, swabs as well as exudates, in Ureaplasma Broth and Agar media immediately on arrival in the laboratory to avoid loss of viability. Swabs and exudates may, if necessary, be inoculated into broth and held no longer than four days at 4°C before incubation. Specimens should be streaked over a wide area of the agar plate. Urine specimens are prepared by centrifugation and the sediment inoculated immediately. Agar cultures are incubated anaerobically at 35-37°C. Cultures of Ureaplasma may show a color change in the plate from yellow to pink as early as 24 hours after incubation. Since viability is limited, Ureaplasma broth cultures should be transplanted as soon as the color change begins to show. This may occur as early as 12 to 16 hours after incubation. Although storage conditions have not been established for all strains or species, most Mycoplasmas and Ureaplasma when fully grown can be stored indefinitely at -80°C. Positive broth cultures must be frozen as soon as possible after indicator change has occurred to preserve viability.



鉴别方法

When seen under the microscope, Ureaplasma colonies produce the manganese reaction turning them dark golden brown or rich deep brown of variable size (depending on crowding). If specimens contain both Mycoplasma hominis and Ureaplasma urealyticum, differentiation may be enhanced by staining agar block preparations with the Dienes stain (6). Ureaplasma colonies retain the brown color, while Mycoplasma hominis and other large colony Mycoplasmas stain azure blue and exhibit the typical "fried egg appearance" due to growth of the organisms into the agar.

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