哥伦比亚血琼脂

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液体哥伦比亚肉汤培养基、哥伦比亚血平板产品基本信息

产品名称: 哥伦比亚培养基、哥伦比亚血琼脂 ; CBA培养基
产品又名:
 哥伦比亚液体培养基、哥伦比亚肉汤培养基、哥伦比亚血琼脂培养基、哥伦比亚血琼脂基础培养基、哥伦比亚血平板培养基、哥伦比亚溶血培养基、哥伦比亚基础培养基、哥伦比亚血琼脂平板培养基、哥伦比亚羊血培养基、哥伦比亚羊血平板培养基

哥伦比亚血平板、哥伦比亚血琼脂培养基平板、哥伦比亚羊血平板、哥伦比亚琼脂培养基
 
英文名称: Columbia Blood Agar;CBA
培养基类型: 加富培养基
品牌: ELITE-MEDIA(艾礼培养基)
产品目录号: M1005-01(哥伦比亚琼脂250g)、M1005-02(哥伦比亚琼脂500g)、M1489-01(哥伦比亚肉汤250g)、M1489-02(哥伦比亚肉汤500g)
产品规格: 20个平板/包、100个平板/箱(9cm,无菌即用)
保存条件: 2-8°C,避光
保质期: 2个月
产品性状: 深红色,不透明凝胶。
注意事项: 避免摄入、呼入、皮肤接触。配制时在通风橱中进行,戴口罩、手套、护目镜。
相关产品: 哥伦比亚琼脂基础
脱纤维绵羊血



哥伦比亚培养基产品描述:

哥伦比亚血琼脂(Columbia Blood Agar)培养基是以哥伦比亚琼脂或胰蛋白胨大豆琼脂(TSA培养基)为培养基基础,加5%脱纤羊血配制而成的。哥伦比亚血琼脂是一种高度营养型的固体培养基,可以用来培养和回收各种苛养和非苛养型微生物,是应用非常广泛的培养基。

哥伦比亚血琼脂高度营养性来自于配方中的特殊混合蛋白胨,酵母提取物则提供B族维生素,刺激微生物的生长,尤其是细菌的生长。羊血提供X因子(亚铁血红素),是很多细菌生长所必须的,但是缺少V因子(烟酰胺腺嘌呤二核苷酸,NAD)。因此,既需要X因子又需要V因子的流感嗜血杆菌不能在哥伦比亚血琼脂培养基生长。该培养基能够支持微生物快速茁壮的生长,还能提供高分辨率的溶血反应,更具特征性的菌落形态特征和更高的色素生成量。



哥伦比亚琼脂培养基用途

临床实验室里常用哥伦比亚血琼脂鉴定溶血类型。但需要注意的是,哥伦比亚学琼脂有相对较高的含糖量,造成链球菌的Beta溶血反应偏绿色,可能误读成Alpha。

哥伦比亚血琼脂培养基本身不具有选择性,加入不同的抑制剂,如抗生素及其组合,就成了具有选择性和鉴别性的培养基,用于各种微生物的分离。



哥伦比亚血琼脂基础培养基配方与配制方法

哥伦比亚琼脂培养基配方:

成分 g/L
特殊蛋白胨 23.0
玉米淀粉 1.0
氯化钠 5.0
琼脂 15.0
脱纤维绵羊血(制作血平板) 50ml
pH 7.3 ± 0.2  

哥伦比亚血平板培养基配制方法: 1. 称取44g 哥伦比亚血琼脂(ISO),加1000mL去离子水重悬。 2. 边加热边搅动,煮沸1min,使其完全溶解。 3. 121°C高温蒸汽灭菌15min。 4. 待冷却至50°C左右时,加入50ml脱纤维羊血,混匀后倒平板。 配制好的培养基应2-8°C避光保存。


实验方法

Elek Test Elek-Test 示意图 即白喉棒状杆菌毒力实验,48h,即可见毒素-抗毒素沉淀线。 实验流程: 1. Mix a tube of melted nutrient agarwith 2 ml of sterile horse serum. 2. Rotate the tube to mix the serum and agar. Do not shake the tube. 3. Pour the mixture into a sterile petri dish. 4. Using lightly flamed forceps, lay the strip of anti-toxin impregnated filter paper across the centre of the petri dish allowing it to sink beneath the agar surface. 5. Allow the agar to set, then lift one corner of the lid and let the plate dry for 30-45 min in the incubator. 6. When dry inoculate with a toxinogenic strain of C. diphtheriae by streaking a single line of inoculum across the plate and paper strip at right angles to the strip. 7. Repeat this about 1 inch away from the C. diphtheriae inoculum with a test strain. 8. Incubate the plate for 24 hrs and observe the results. 实验结果: After 24 hours of incubation at 37°C, plate is examined with transmitted light for the presence of fine precipitin lines at 45 degree angle to the streaks. Positive Test: Precipitin lines form at zone of equivalence, test organism is toxigenic. Nagler Test(Nagler Reaction) 是产气荚膜梭菌诊断实验。 100ml哥伦比亚学琼脂中加入5mlFildes提取物,10ml卵黄乳液,100-125mg/L新霉素,产气荚膜梭菌抗毒素。 产气荚膜梭菌鉴定Nagleer-reaction示意图 实验流程: 1. Label and dry a egg yolk media plate and mark the plate into two halves. 2. Inoculate 60 µl of Clostridium perfringens type A antitoxin in half of the plate, spread over the surface of agar using a spreader and allow to absorb and dry. 3. Mark the side of the plate in which the antitoxin is inoculated. 4. Streak the test organism in a straight line from the toxin free agar half of the plate to toxin containing side. Repeat the same procedure with control strains on the same plate. 5. Incubate anaerobically at 35-37°C for 24-48 hrs. 6. Examine the plate for an opalescent halo around the inoculum and inhibition by antitoxin. 结果与分析: 产气荚膜梭菌Naglers-reaction结果

CAMP Test 初步鉴定无乳链球菌,将无乳链球菌与其它Beta溶血B群链球菌区别开来。初步鉴定单增李斯特菌。

A. Standard CAMP test 1. Using an inoculating loop, streak a beta-lysin-producing Staphylococcus aureus (ATCC25923) in a straight line across the center of a sheep blood agar plate. 2. Streak test organism in a straight line perpendicular to the S. aureus leaving 1cm space between the two streaks. (Multiple organisms can be tested on a single plate if they are 3 to 4mm apart). 3. Incubate the plate at 37 degree Celsius in ambient air for 18-24 hours. B. CAMP Spot test 1. Remove test kit from the freezer and allow it to thaw. 2. Using a pipette, place one drop of CAMP spot test reagent next to a characteristic colony grown for 18-24 hours on blood agar plate. 3. Incubate the plate aerobically or in 5-10% CO2 at 37°C for 20-30 minutes. 4. Observe, using transmitted light, for an arc or circle of enhanced hemolysis next to the colony. If reaction is negative, re-incubate for an additional 30 minutes.

Reversed CAMP Test 是高度灵敏性和专一性的产气荚膜梭菌检测实验。与Nagler Test作用相同,可相互替换。

液体哥伦比亚肉汤培养基、哥伦比亚血平板产品说明书pdf版和相关资料下载

液体哥伦比亚肉汤培养基、哥伦比亚血平板产品应用举例

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