| 质粒名称: | pcdna3.1 hciap1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 8311 |
| 存储实验室: | Jon Ashwell |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 7358 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | cIAP1 / BIRC2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1700 bp |
| 标签/融合蛋白: | myc (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | T7 |
| 3’测序引物 | SP6 |
|
文献引用方法 |
|
| 材料和方法部分: |
pcdna3.1 hciap1 was a gift from Jon Ashwell (Addgene plasmid # 8311 ; http://n2t.net/addgene:8311 ; RRID:Addgene_8311)
|
| 参考文献部分: | TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2. Li X, Yang Y, Ashwell JD. Nature 2002 Mar 21;416(6878):345-7. 10.1038/416345a PubMed 11907583 |
买家导航
pcdna3.1 hciap1载体质粒基本信息
pcdna3.1 hciap1质粒图谱载体图谱和pcdna3.1 hciap1载体序列质粒序列多克隆位点信息
pcdna3.1 hciap1质粒载体简介
pcdna3.1 hciap1质粒序列载体序列
LOCUS pcdna3.1 hciap1 7358 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcdna3.1 hciap1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7358)
AUTHORS Li X, Yang Y, Ashwell JD
TITLE TNF-RII and c-IAP1 mediate ubiquitination and degradation of TRAF2.
JOURNAL Nature 2002 Mar 21;416(6878):345-7.
PUBMED 11907583
REFERENCE 2 (bases 1 to 7358)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7358
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 120..499
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 500..703
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 654..674
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 748..767
/label=T7
/note="T7 promoter, forward primer"
promoter 748..766
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 822..851
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(2796..2814)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2797..2814)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(2834..2851)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2840..3064
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3110..3538
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3197..3216)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3407..3428
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3547..3567)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3552..3881
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3732..3867
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3794..3813
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3948..4742
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4002..4021)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4612..4631
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4916..5037
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4953..4972)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5007..5026
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5086..5102)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5086..5102)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5099..5121)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5110..5126
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5134..5164)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5179..5200
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5317..5334)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5488..6076)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5568..5587)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6247..7107)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6870..6889
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7108..7212)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7287..7306)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
61 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
121 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
481 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
541 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
601 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
661 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
721 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
781 gtaccgagct cggatccccc gggctgcagg aattcacaat ggagcagaag ctgatttccg
841 aggaggacct gggggccatg cacaaaactg cctcccaaag acttttccca ggtccctcgt
901 atcaaaacat taagagtata atggaagata gcacgatctt gtcagattgg acaaacagca
961 acaaacaaaa aatgaagtat gacttttcct gtgaactcta cagaatgtct acatattcaa
1021 ctttccccgc cggggtgcct gtctcagaaa ggagtcttgc tcgtgctggt ttttattata
1081 ctggtgtgaa tgacaaggtc aaatgcttct gttgtggcct gatgctggat aactggaaac
1141 taggagacag tcctattcaa aagcataaac agctatatcc tagctgtagc tttattcaga
1201 atctggtttc agctagtctg ggatccacct ctaagaatac gtctccaatg agaaacagtt
1261 ttgcacattc attatctccc accttggaac atagtagctt gttcagtggt tcttactcca
1321 gcctttctcc aaaccctctt aattctagag cagttgaaga catctcttca tcgaggacta
1381 acccctacag ttatgcaatg agtactgaag aagccagatt tcttacctac catatgtggc
1441 cattaacttt tttgtcacca tcagaattgg caagagctgg tttttattat ataggacctg
1501 gagatagggt agcctgcttt gcctgtggtg ggaagctcag taactgggaa ccaaaggatg
1561 atgctatgtc agaacaccgg aggcattttc ccaactgtcc atttttggaa aattctctag
1621 aaactctgag gtttagcatt tcaaatctga gcatgcagac acatgcagct cgaatgagaa
1681 catttatgta ctggccatct agtgttccag ttcagcctga gcagcttgca agtgctggtt
1741 tttattatgt gggtcgcaat gatgatgtca aatgcttttg ttgtgatggt ggcttgaggt
1801 gttgggaatc tggagatgat ccatgggtag aacatgccaa gtggtttcca aggtgtgagt
1861 tcttgatacg aatgaaaggc caagagtttg ttgatgagat tcaaggtaga tatcctcatc
1921 ttcttgaaca gctgttgtca acttcagata ccactggaga agaaaatgct gacccaccaa
1981 ttattcattt tggacctgga gaaagttctt cagaagatgc tgtcatgatg aatacacctg
2041 tggttaaatc tgccttggaa atgggcttta atagagacct ggtgaaacaa acagttcaaa
2101 gtaaaatcct gacaactgga gagaactata aaacagttaa tgatattgtg tcagcacttc
2161 ttaatgctga agatgaaaaa agagaagagg agaaggaaaa acaagctgaa gaaatggcat
2221 cagatgattt gtcattaatt cggaagaaca gaatggctct ctttcaacaa ttgacatgtg
2281 tgcttcctat cctggataat cttttaaagg ccaatgtaat taataaacag gaacatgata
2341 ttattaaaca aaaaacacag atacctttac aagcgagaga actgattgat accattttgg
2401 ttaaaggaaa tgctgcggcc aacatcttca aaaactgtct aaaagaaatt gactctacat
2461 tgtataagaa cttatttgtg gataagaata tgaagtatat tccaacagaa gatgtttcag
2521 gtctgtcact ggaagaacaa ttgaggaggt tgcaagaaga acgaacttgt aaagtgtgta
2581 tggacaaaga agtttctgtt gtatttattc cttgtggtca tctggtagta tgccaggaat
2641 gtgccccttc tctaagaaaa tgccctattt gcaggggtat aatcaagggt actgttcgta
2701 catttctctc ttaaggtcac cagatctagt aagccgaatt ctgcagatat ccatcacact
2761 ggcggccgct cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
2821 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
2881 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
2941 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
3001 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
3061 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
3121 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
3181 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
3241 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
3301 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
3361 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
3421 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
3481 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
3541 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
3601 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
3661 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
3721 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
3781 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
3841 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
3901 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
3961 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
4021 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
4081 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
4141 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
4201 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
4261 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
4321 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
4381 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
4441 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
4501 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
4561 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
4621 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
4681 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
4741 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
4801 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
4861 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
4921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
4981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
5041 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
5101 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
5161 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
5221 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
5281 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5341 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5401 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
5461 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5521 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5581 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5641 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5701 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
5761 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5821 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5881 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5941 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
6001 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
6061 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
6121 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6181 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6241 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6301 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6361 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6421 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6481 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6541 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6601 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6661 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6721 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6781 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6841 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6901 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6961 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
7021 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
7081 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
7141 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7201 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
7261 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
7321 tatctgctcc ctgcttgtgt gttggaggtc gctgagta
//
pcdna3.1 hciap1载体图谱质粒图谱pdf版和pcdna3.1 hciap1载体序列质粒序列等相关资料下载
pcdna3.1 hciap1质粒载体应用举例
- 上一篇：plRL2
- 下一篇：EGFP-Rubicon
