| 质粒名称: | pRRLsin-SV40 T antigen-IRES-mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 58993 |
| 存储实验室: | Snorri Thorgeirsson |
|
载体骨架基本信息 |
|
| 载体名称: | pRRLsin.cPPT.PGK/GFP.WPRE |
| 载体出品公司: | -- |
| 空载体大小: | 6110 bp |
| 完整质粒大小: | 11405 bp |
| 载体修饰: | 位于nt 924的NotI站点被删除 |
| 载体类型: | 哺乳细胞表达,慢病毒 |
| 筛选标记: | mCherry 红色荧光 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CMV enhancer-chicken beta actin promoter |
| 物种来源: | -- |
| 插入大小: | 1730 bp |
| 基因突变: | MCS 中的 EcoRV 站点转换为 BamHI 站点 |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SalI（克隆期间销毁） |
| 3’克隆位点: | BamHI(未破坏) |
| 5’测序引物 | -- |
| 3’测序引物 | pCAG-F |
|
文献引用方法 |
|
| 材料和方法部分: | pRRLsin-SV40 T antigen-IRES-mCherry was a gift from Snorri Thorgeirsson (Addgene plasmid # 58993 ; http://n2t.net/addgene:58993 ; RRID:Addgene_58993) |
| 参考文献部分: | Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types. Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS. Gastroenterology. 2013 Jul;145(1):221-31. doi: 10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19. 10.1053/j.gastro.2013.03.013 PubMed 23523670 |
买家导航
pRRLsin-SV40 T antigen-IRES-mCherry载体质粒基本信息
pRRLsin-SV40 T antigen-IRES-mCherry质粒图谱载体图谱和pRRLsin-SV40 T antigen-IRES-mCherry载体序列质粒序列多克隆位点信息
pRRLsin-SV40 T antigen-IRES-mCherry质粒载体简介
pRRLsin-SV40 T antigen-IRES-mCherry质粒序列载体序列
LOCUS pRRLsin-SV40 T antigen-IRES-mCherry 11418 bp ds-DNA circular SYN 08-SEP-2021
DEFINITION lentiviral expression of SV40 large T antigen and mCherry red
fluorescent reporter.
ACCESSION .
VERSION .
KEYWORDS pRRLsin-SV40 T antigen-IRES-mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11418)
AUTHORS Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE,
Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS
TITLE Modeling pathogenesis of primary liver cancer in lineage-specific
mouse cell types.
JOURNAL Gastroenterology. 2013 Jul;145(1):221-31. doi:
10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19.
PUBMED 23523670
REFERENCE 2 (bases 1 to 11418)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 8, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11418
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 8..234
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 235..415
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 462..587
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1085..1318
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1503..1547
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1814..1931
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1953..2332
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 2611..3627
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 3551..3573
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 3635..3654
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 3726..5852
/codon_start=1
/product="large T antigen from SV40 (simian virus 40)"
/label=large T antigen
/translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLF
CSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYT
TKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLI
CKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETK
CDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQA
VDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLP
KMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLN
FELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKR
TQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALL
LMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDD
DEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFT
CFKKPPTPPPEPET"
misc_feature 5921..6507
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(6088..6105)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 6315..6334
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 6508..7218
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(6653..6671)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 6921..6940
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind complement(7238..7254)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7238..7254)
/label=pBluescriptSK
/note="For pBluescript vector"
misc_feature 7269..7857
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7322..7342)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7740..7751)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7944..8177
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 8249..8370
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8286..8305)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8340..8359
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 8410..8545
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8472..8491
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(8565..8584)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8566..8584)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(8594..8611)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(8594..8610)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8603..8625)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 8752..9207
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8839..8858)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9049..9070
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 9233..9337
/gene="bla"
/label=AmpR promoter
CDS 9338..10198
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9556..9575)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10369..10957
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10858..10877
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11111..11128
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 11245..11266
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11281..11311
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11319..11335
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11324..11346
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11343..11359
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11343..11359
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 11378..11398
/label=T3
/note="T3 promoter, forward primer"
promoter 11380..11398
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
901 aaagtaagac caccgcacag caagcggcct ggccgctgat cttcagacct ggaggaggag
961 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
1021 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
1081 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
1141 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
1201 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
1261 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
1321 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
1381 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
1441 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
1501 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
1561 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
1621 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
1681 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
1741 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
1801 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
1861 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
1921 ttcaaaattt tatcgatcac gagactagcc tcgacattga ttattgacta gttattaata
1981 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
2041 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
2101 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
2161 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
2221 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
2281 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
2341 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
2401 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggnsgcgcg
2461 ccrggsgggg sggggsgggg sgrggggsgg ggsggggsgr ggsggrgagg tgcggcggca
2521 gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg
2581 ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct tcgccccgtg
2641 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
2701 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
2761 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg
2821 tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc
2881 ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
2941 cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc cgcggtgcgg ggggggctgc
3001 gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg gggtgtgggc
3061 gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc gagttgctga gcacggcccg
3121 gcttcgggtg cggggctccg tacggggcgt ggcgcggggc tcgccgtgcc gggcgggggg
3181 tggcggcagg tgggggtgcc gggcggggcg gggccgcctc gggccggggr gggctcgggg
3241 grggggcgcg gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat
3301 tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg
3361 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc
3421 ggcgccggca ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc
3481 cttctccctc tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg
3541 ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat
3601 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
3661 catcattttg gcaaagaatt cctcgaggat gaggatcccg cgaccggtcg accagctttg
3721 caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
3781 ttgaaaggag tgcctggggg aatattcctc tgatgagaaa ggcatattta aaaaaatgca
3841 aggagtttca tcctgataaa ggaggagatg aagaaaaaat gaagaaaatg aatactctgt
3901 acaagaaaat ggaagatgga gtaaaatatg ctcatcaacc tgactttgga ggcttctggg
3961 atgcaactga gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
4021 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
4081 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
4141 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
4201 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
4261 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
4321 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
4381 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
4441 atccattttc tgttattgag gaaagtttgc caggtgggtt aaaggagcat gattttaatc
4501 cagaagaagc agaggaaact aaacaagtgt cctggaagct tgtaacagag tatgcaatgg
4561 aaacaaaatg tgatgatgtg ttgttattgc ttgggatgta cttggaattt cagtacagtt
4621 ttgaaatgtg tttaaaatgt attaaaaaag aacagcccag ccactataag taccatgaaa
4681 agcattatgc aaatgctgct atatttgctg acagcaaaaa ccaaaaaacc atatgccaac
4741 aggctgttga tactgtttta gctaaaaagc gggttgatag cctacaatta actagagaac
4801 aaatgttaac aaacagattt aatgatcttt tggataggat ggatataatg tttggttcta
4861 caggctctgc tgacatagaa gaatggatgg ctggagttgc ttggctacac tgtttgttgc
4921 ccaaaatgga ttcagtggtg tatgactttt taaaatgcat ggtgtacaac attcctaaaa
4981 aaagatactg gctgtttaaa ggaccaattg atagtggtaa aactacatta gcagctgctt
5041 tgcttgaatt atgtgggggg aaagctttaa atgttaattt gcccttggac aggctgaact
5101 ttgagctagg agtagctatt gaccagtttt tagtagtttt tgaggatgta aagggcactg
5161 gaggggagtc cagagatttg ccttcaggtc agggaattaa taacctggac aatttaaggg
5221 attatttgga tggcagtgtt aaggtaaact tagaaaagaa acacctaaat aaaagaactc
5281 aaatatttcc ccctggaata gtcaccatga atgagtacag tgtgcctaaa acactgcagg
5341 ccagatttgt aaaacaaata gattttaggc ccaaagatta tttaaagcat tgcctggaac
5401 gcagtgagtt tttgttagaa aagagaataa ttcaaagtgg cattgctttg cttcttatgt
5461 taatttggta cagacctgtg gctgagtttg ctcaaagtat tcagagcaga attgtggagt
5521 ggaaagagag attggacaaa gagtttagtt tgtcagtgta tcaaaaaatg aagtttaatg
5581 tggctatggg aattggagtt ttagattggc taagaaacag tgatgatgat gatgaagaca
5641 gccaggaaaa tgctgataaa aatgaagatg gtggggagaa gaacatggaa gactcagggc
5701 atgaaacagg cattgattca cagtcccaag gctcatttca ggcccctcag tcctcacagt
5761 ctgttcatga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
5821 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacggg
5881 gatcctcatc gcggccgcta cgtaaattcc gccccccccc cccctctccc tccccccccc
5941 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
6001 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
6061 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
6121 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
6181 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
6241 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
6301 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
6361 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
6421 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
6481 aaacacgatg ataatatggc cacaaccatg gtgagcaagg gcgaggagga taacatggcc
6541 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
6601 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
6661 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
6721 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
6781 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
6841 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
6901 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
6961 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
7021 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
7081 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
7141 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
7201 gacgagctgt acaagtaaga attcctgcag cccgggggat ccactagttc tagagcggcc
7261 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
7321 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
7381 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
7441 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
7501 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
7561 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
7621 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
7681 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
7741 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
7801 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
7861 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
7921 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
7981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
8041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
8101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
8161 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
8221 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
8281 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
8341 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
8401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
8461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
8521 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
8581 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
8641 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
8701 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
8761 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
8821 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
8881 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
8941 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
9001 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
9061 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
9121 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
9181 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
9241 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
9301 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
9361 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
9421 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
9481 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
9541 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
9601 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
9661 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
9721 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
9781 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
9841 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
9901 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
9961 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
10021 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
10081 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
10141 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
10201 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
10261 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
10321 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
10381 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
10441 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
10501 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
10561 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
10621 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
10681 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
10741 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
10801 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
10861 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
10921 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
10981 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
11041 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
11101 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
11161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
11221 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
11281 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
11341 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
11401 acaaaagctg gagctgca
//
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pRRLsin-SV40 T antigen-IRES-mCherry质粒载体应用举例
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