| 质粒名称: | pLVX-Tet3G blasticidin |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 128061 |
| 存储实验室: | Oskar Laur |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-Tet3G |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-Tet3G blasticidin was a gift from Oskar Laur (Addgene plasmid # 128061 ; http://n2t.net/addgene:128061 ; RRID:Addgene_128061) |
买家导航
pLVX-Tet3G blasticidin载体质粒基本信息
pLVX-Tet3G blasticidin质粒图谱载体图谱和pLVX-Tet3G blasticidin载体序列质粒序列多克隆位点信息
pLVX-Tet3G blasticidin质粒载体简介
pLVX-Tet3G blasticidin质粒序列载体序列
LOCUS pLVX-Tet3G blasticidin 8606 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector encoding the Tet-On 3G transactivator protein,
which allows for inducible gene expression. With Blasticidin
resistance..
ACCESSION .
VERSION .
KEYWORDS pLVX-Tet3G blasticidin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8606)
TITLE Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE 2 (bases 1 to 8606)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8606
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2659..2679
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2705..2729
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2821..3567
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
misc_feature 3576..4149
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3743..3760)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3970..3989
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4163..4561
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 4575..5163
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4628..4648)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5046..5057)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5370..6003
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(6131..6147)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6131..6147)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6144..6166)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6155..6171
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6179..6209)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6224..6245
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6362..6379)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6533..7121)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6613..6632)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7292..8152)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7915..7934
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8153..8257)
/gene="bla"
/label=AmpR promoter
polyA_signal 8305..8439
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8342..8361)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8396..8415
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgaggccacc
2821 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatggagtc
2881 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
2941 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
3001 gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
3061 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
3121 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
3181 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
3241 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
3301 cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag
3361 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
3421 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
3481 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
3541 tttgaccttg acatgctccc cgggtaagga tcccgcccct ctccctcccc cccccctaac
3601 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
3661 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
3721 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
3781 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
3841 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
3901 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
3961 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
4021 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
4081 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
4141 cgatgataag cttgccacaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
4201 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
4261 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
4321 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
4381 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
4441 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
4501 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggctg
4561 aacgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
4621 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
4681 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
4741 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
4801 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
4861 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
4921 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
4981 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
5041 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
5101 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
5161 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
5221 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
5281 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
5341 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
5401 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
5461 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
5521 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
5581 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
5641 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
5701 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
5761 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
5821 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
5881 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
5941 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
6001 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
6061 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
6121 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
6181 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
6241 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
6301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
6361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
6421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
6481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
6541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
6601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
6661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
6721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
6781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
6841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
6901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
6961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
7021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
7081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
7141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
7201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
7261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
7321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
7381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
7441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
7501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
7561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
7621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
7681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
7741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
7801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
7861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
7921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
7981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
8041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
8101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
8161 tttttctata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
8221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
8281 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
8341 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
8401 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
8461 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
8521 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
8581 taaatatgta ctacaaactt agtagt
//
pLVX-Tet3G blasticidin载体图谱质粒图谱pdf版和pLVX-Tet3G blasticidin载体序列质粒序列等相关资料下载
pLVX-Tet3G blasticidin质粒载体应用举例
- 上一篇：pHDM-Tat1b
- 下一篇：DCT.VV.Orf196
