| 质粒名称: | pHR CD19-FcGamma CAR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 113014 |
| 存储实验室: | Ron Vale |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CD19 CAR-P intracellular GFP |
| 物种来源: | H. sapiens (human), M. musculus (mouse), Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | GFP |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR CD19-FcGamma CAR was a gift from Ron Vale (Addgene plasmid # 113014 ; http://n2t.net/addgene:113014 ; RRID:Addgene_113014) |
| 参考文献部分: | Chimeric antigen receptors that trigger phagocytosis. Morrissey MA, Williamson AP, Steinbach AM, Roberts EW, Kern N, Headley MB, Vale R. Elife. 2018 Jun 4;7. pii: 36688. doi: 10.7554/eLife.36688. 36688 [pii] PubMed 29862966 |
买家导航
pHR CD19-FcGamma CAR载体质粒基本信息
pHR CD19-FcGamma CAR质粒图谱载体图谱和pHR CD19-FcGamma CAR载体序列质粒序列多克隆位点信息
pHR CD19-FcGamma CAR质粒载体简介
pHR CD19-FcGamma CAR质粒序列载体序列
LOCUS pHR CD19-FcGamma CAR 10820 bp ds-DNA circular SYN 21-JUL-2020
DEFINITION for production of lentivirus encoding the CD19 CAR-Pfcgamma.
ACCESSION .
VERSION .
KEYWORDS pHR CD19-FcGamma CAR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10820)
AUTHORS Morrissey MA, Williamson AP, Steinbach AM, Roberts EW, Kern N,
Headley MB, Vale R
TITLE Chimeric antigen receptors that trigger phagocytosis.
JOURNAL Elife. 2018 Jun 4;7. pii: 36688. doi: 10.7554/eLife.36688.
PUBMED 29862966
REFERENCE 2 (bases 1 to 10820)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 21, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10820
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 127..534
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
regulatory 568..577
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1711..2430
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1756..1777)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2017..2036)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2364..2385
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 2491..3079
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2544..2564)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2962..2973)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(3082..3098)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3083..3099)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 3189..3422
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(3447..3465)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3448..3465)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3755..3774)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(3988..4007)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4107..4129
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4167..4185)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4253..4357
/gene="bla"
/label=AmpR promoter
CDS 4358..5218
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4576..4595)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5389..5977
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5878..5897
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6131..6148
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(6218..6238)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6223..6552
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6403..6538
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6465..6484
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 7686..7751
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 7881..7901
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 8326..8460
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8363..8382)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8417..8436
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 8628..9261
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 9308..9433
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9930..10163
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10677..10794
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 tccagtttgg atcgataagc ttgatatcga attcctgcag ccccgataaa ataaaagatt
61 ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta
121 gctgcagtaa cgccattttg caaggcatgg aaaaatacca aaccaagaat agagaagttc
181 agatcaaggg cgggtacatg aaaatagcta acgttgggcc aaacaggata tctgcggtga
241 gcagtttcgg ccccggcccg gggccaagaa cagatggtca ccgcagtttc ggccccggcc
301 cgaggccaag aacagatggt ccccagatat ggcccaaccc tcagcagttt cttaagaccc
361 atcagatgtt tccaggctcc cccaaggacc tgaaatgacc ctgcgcctta tttgaattaa
421 ccaatcagcc tgcttctcgc ttctgttcgc gcgcttctgc ttcccgagct ctataaaaga
481 gctcacaacc cctcactcgg cgcgccagtc ctccgacaga ctgagtcgcc cgggggggat
541 ctggagctct cgagaattct cacgcgtgcc accatggcct taccagtgac cgccttgctc
601 ctgccgctgg ccttgctgct ccacgccgcc aggccggaca tccagatgac acagactaca
661 tcctccctgt ctgcctctct gggagacaga gtcaccatca gttgcagggc aagtcaggac
721 attagtaaat atttaaattg gtatcagcag aaaccagatg gaactgttaa actcctgatc
781 taccatacat caagattaca ctcaggagtc ccatcaaggt tcagtggcag tgggtctgga
841 acagattatt ctctcaccat tagcaacctg gagcaagaag atattgccac ttacttttgc
901 caacagggta atacgcttcc gtacacgttc ggagggggga ccaagctgga gatcacaggt
961 ggcggtggct cgggcggtgg tgggtcgggt ggcggcggat ctgaggtgaa actgcaggag
1021 tcaggacctg gcctggtggc gccctcacag agcctgtccg tcacatgcac tgtctcaggg
1081 gtctcattac ccgactatgg tgtaagctgg attcgccagc ctccacgaaa gggtctggag
1141 tggctgggag taatatgggg tagtgaaacc acatactata attcagctct caaatccaga
1201 ctgaccatca tcaaggacaa ctccaagagc caagttttct taaaaatgaa cagtctgcaa
1261 actgatgaca cagccattta ctactgtgcc aaacattatt actacggtgg tagctatgct
1321 atggactact ggggccaagg aacctcagtc accgtctcct caaccacgac gccagcgccg
1381 cgaccaccaa caccggcgcc caccatcgcg tcgcagcccc tgtccctgcg cccagaggcg
1441 tgccggccag cggcgggggg cgcagtgcac acgagggggc tggacttcgc ctgtgatatc
1501 tacatctggg cgcccttggc cgggacttgt ggggtccttc tcctgtcact ggttatcacc
1561 ctttactgca gacgactcaa gatccaggtc cgaaaggcag ctatagccag ccgtgagaaa
1621 gcagatgctg tctacacggg cctgaacacc cggagccagg agacatatga gactctgaag
1681 catgagaaac caccccaggg atccggaagt atggtgagca agggcgagga gctgttcacc
1741 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
1801 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
1861 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
1921 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
1981 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
2041 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
2101 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
2161 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
2221 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
2281 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
2341 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
2401 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagagt cgacctgcag
2461 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
2521 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
2581 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
2641 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
2701 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
2761 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
2821 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
2881 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
2941 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
3001 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
3061 ctttgggccg cctccccgca tcgataccgt cgacctcgag ggaattaatt cgagctcggt
3121 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
3181 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
3241 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
3301 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
3361 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
3421 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
3481 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
3541 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
3601 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
3661 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
3721 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
3781 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
3841 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
3901 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
3961 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
4081 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
4141 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
4201 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
4261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
4321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
4381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
4441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
4501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
4561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
4621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
4681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
4741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
4801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
4861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
4921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
4981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
5101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
5161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
5221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
5281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
5341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
5401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
5461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
5521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
5581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
5641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
5701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
5761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
5821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
5881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
5941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
6001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
6061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
6121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
6181 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
6241 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
6301 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
6361 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
6421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
6481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
6541 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
6601 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
6661 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
6721 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
6781 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
6841 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
6901 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
6961 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
7021 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
7081 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
7141 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
7201 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
7261 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
7321 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
7381 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
7441 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
7501 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
7561 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
7621 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
7681 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
7741 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
7801 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
7861 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
7921 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
7981 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
8041 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
8101 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
8161 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
8221 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
8281 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
8341 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
8401 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
8461 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
8521 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
8581 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
8641 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
8701 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
8761 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
8821 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
8881 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
8941 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
9001 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
9061 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
9121 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
9181 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
9241 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
9301 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
9361 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
9421 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
9481 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
9541 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
9601 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
9661 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
9721 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg
9781 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
9841 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
9901 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
9961 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
10021 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
10081 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
10141 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
10201 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
10261 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
10321 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
10381 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
10441 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
10501 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
10561 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
10621 attcgattag tgaacggatc tcgacggtat cgccaaatgg cagtattcat ccacaatttt
10681 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
10741 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
10801 tattacaggg acagcagaga
//
