pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

LOCUS       pC0072	LbuCas13a His6-TwinStrep-SUMO-BsaI                9561 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses LbuCas13a from a SUMO-tagged bacterial expression 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    pC0072	LbuCas13a His6-TwinStrep-SUMO-BsaI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9561)
  AUTHORS   Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang 
            F
  TITLE     Multiplexed and portable nucleic acid detection platform with Cas13,
            Cas12a, and Csm6.
  JOURNAL   Science. 2018 Apr 27;360(6387):439-444. doi: 
            10.1126/science.aaq0179. Epub 2018 Feb 15.
  PUBMED    29449508
REFERENCE   2  (bases 1 to 9561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             138..221
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             222..512
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     primer_bind     complement(4043..4061)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4057..4104
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(4279..4307)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4334..4352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4420..4524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4525..5385
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4743..4762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5556..6144
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6045..6064
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6298..6315
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6330..6469
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6395..6414)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6555..6577
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6571..6762)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8074..9156)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9118..9137
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9157..9234)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9440..9459
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     9543..1
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9543..9561
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      121 gcagccatat ggctagctgg agccatccgc agtttgaaaa aggtggtggt agcggtggtg
      181 gttcaggtgg tagtgcatgg tcacaccctc agtttgagaa aatgtcggac tcagaagtca
      241 atcaagaagc taagccagag gtcaagccag aagtcaagcc tgagactcac atcaatttaa
      301 aggtgtccga tggatcttca gagatcttct tcaagatcaa aaagaccact cctttaagaa
      361 ggctgatgga agcgttcgct aaaagacagg gtaaggaaat ggactcctta agattcttgt
      421 acgacggtat tagaatccaa gctgatcaga cccctgaaga tttggacatg gaggataacg
      481 atattattga ggctcacaga gaacagattg gtggatctat gaaggtgaca aaagtgggcg
      541 gcatcagcca caagaagtat acctccgagg gcaggctggt gaagtctgag agcgaggaga
      601 acaggaccga cgagcgcctg tccgccctgc tgaatatgag gctggatatg tatatcaaga
      661 accccagctc caccgagaca aaggagaatc agaagcgcat cggcaagctg aagaagttct
      721 tttccaacaa gatggtgtat ctgaaggaca atacactgtc tctgaagaat ggcaagaagg
      781 agaacatcga ccgggagtac tctgagacag atatcctgga gagcgacgtg agagataaga
      841 agaacttcgc cgtgctgaag aagatctacc tgaatgagaa cgtgaacagc gaggagctgg
      901 aggtgttccg gaatgatatc aagaagaagc tgaacaagat caattctctg aagtacagct
      961 ttgagaagaa caaggccaat tatcagaaga tcaacgagaa caatatcgag aaggtggagg
     1021 gcaagagcaa gagaaatatc atctacgact actataggga gtccgccaag cgggacgcct
     1081 acgtgagcaa cgtgaaggag gcctttgaca agctgtataa ggaggaggat atcgccaagc
     1141 tggtgctgga gatcgagaat ctgaccaagc tggagaagta caagatcagg gagttctatc
     1201 acgagatcat cggccgcaag aatgacaagg agaactttgc caagatcatc tacgaggaga
     1261 tccagaacgt gaacaatatg aaggagctga tcgagaaggt gcctgatatg tccgagctga
     1321 agaagtctca ggtgttctat aagtactatc tggacaagga ggagctgaac gataagaata
     1381 tcaagtacgc cttctgccac tttgtggaga tcgagatgag ccagctgctg aagaactacg
     1441 tgtataagag gctgtccaac atctctaatg acaagatcaa gcgcatcttt gagtatcaga
     1501 atctgaagaa gctgatcgag aataagctgc tgaacaagct ggatacatac gtgagaaatt
     1561 gtggcaagta taactactat ctgcaggacg gcgagatcgc cacctccgat ttcatcgccc
     1621 ggaacagaca gaatgaggcc tttctgcgga acatcatcgg cgtgtctagc gtggcctact
     1681 tctctctgag aaatatcctg gagacagaaa acgagaatga catcacaggc aggatgcgcg
     1741 gcaagaccgt gaagaacaat aagggcgagg agaagtacgt gtctggcgag gtggataaga
     1801 tctacaacga gaacaagaag aatgaggtga aggagaacct gaagatgttc tacagctatg
     1861 actttaacat ggataacaag aatgagatcg aggacttctt tgccaatatc gatgaggcca
     1921 tctcctctat caggcacggc atcgtgcact tcaacctgga gctggagggc aaggacatct
     1981 tcgcctttaa gaatatcgcc ccaagcgaga tctccaagaa gatgtttcag aacgagatca
     2041 atgagaagaa gctgaagctg aagatcttcc ggcagctgaa ctccgccaac gtgttcagat
     2101 acctggagaa gtataagatc ctgaactacc tgaagcggac aagattcgag tttgtgaaca
     2161 agaatatccc cttcgtgcct tcttttacca agctgtatag ccgcatcgac gatctgaaga
     2221 actctctggg catctactgg aaaaccccta agacaaacga cgataataag acaaaggaga
     2281 tcatcgacgc ccagatctat ctgctgaaga acatctacta tggcgagttc ctgaattact
     2341 ttatgtctaa caatggcaac ttctttgaga tcagcaagga gatcatcgag ctgaacaaga
     2401 atgacaagag gaacctgaaa accggcttct acaagctgca gaagtttgag gatatccagg
     2461 agaagatccc aaaggagtac ctggccaata tccagtccct gtatatgatc aacgccggca
     2521 atcaggacga ggaggagaag gacacataca tcgatttcat ccagaagatc ttcctgaagg
     2581 gctttatgac ctacctggcc aacaatggcc gcctgtccct gatctatatc ggctctgacg
     2641 aggagacaaa cacaagcctg gccgagaaga agcaggagtt cgataagttt ctgaagaagt
     2701 acgagcagaa caataacatc aagatcccct atgagatcaa tgagttcctg agggagatca
     2761 agctgggcaa catcctgaag tacacagagc gcctgaatat gttttatctg atcctgaagc
     2821 tgctgaatca caaggagctg accaacctga agggcagcct ggagaagtac cagtccgcca
     2881 acaaggagga ggccttctcc gaccagctgg agctgatcaa cctgctgaat ctggataata
     2941 acagggtgac agaggacttt gagctggagg ccgatgagat cggcaagttc ctggacttta
     3001 acggcaataa ggtgaaggat aacaaggagc tgaagaagtt cgacaccaat aagatctact
     3061 ttgatggcga gaacatcatc aagcaccgcg ccttctacaa tatcaagaag tatggcatgc
     3121 tgaacctgct ggagaagatc gccgacaagg ccggctacaa gatcagcatc gaggagctga
     3181 agaagtattc caacaagaag aatgagatcg agaagaatca caagatgcag gagaacctgc
     3241 accggaagta tgccaggcca cgcaaggatg agaagttcac agacgaggat tacgagagct
     3301 ataagcaggc catcgagaac atcgaggagt acacccacct gaagaataag gtggagttta
     3361 acgagctgaa tctgctgcag ggcctgctgc tgagaatcct gcacagactg gtgggctaca
     3421 catccatctg ggagcgggac ctgcggttca gactgaaggg cgagtttccc gagaaccagt
     3481 atatcgagga gatcttcaac tttgagaata agaagaacgt gaagtacaag ggcggccaga
     3541 tcgtggagaa gtacatcaag ttctataagg agctgcacca gaatgacgag gtgaagatca
     3601 acaagtatag ctccgccaat atcaaggtgc tgaagcagga gaagaaggat ctgtatatcc
     3661 ggaactatat cgcccacttt aattacatcc ctcacgccga gatcagcctg ctggaggtgc
     3721 tggagaacct gcggaagctg ctgagctatg acagaaagct gaagaatgcc gtgatgaagt
     3781 ccgtggtgga tatcctgaag gagtacggct tcgtggccac atttaagatc ggcgccgaca
     3841 agaagatcgg catccagacc ctggagtctg agaagatcgt gcacctgaag aacctgaaga
     3901 agaagaagct gatgaccgat agaaatagcg aggagctgtg caagctggtg aagatcatgt
     3961 tcgagtacaa gatggaggag aagaagtccg agaactaagg ccgcactcga ggcccgaaag
     4021 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     4081 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga
     4141 ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga
     4201 ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta
     4261 actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct
     4321 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4741 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5041 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5221 tctggagccg gtgagcgtgg gtcacgcggt atcattgcag cactggggcc agatggtaag
     5281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6301 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     6361 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     6421 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     6481 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     6541 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     6601 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     6661 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     6721 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     6781 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     6841 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     6901 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     6961 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     7021 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     7081 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     7141 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     7201 cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg
     7261 cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc
     7321 attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc
     7381 gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac
     7441 gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg
     7501 ctcgccgagg cggcataaat cgccgtgacg atcagcggtc caatgatcga agttaggctg
     7561 gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac
     7621 agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg
     7681 aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc
     7741 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     7801 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     7861 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     7921 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     7981 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     8041 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8101 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8161 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     8221 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     8281 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     8341 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     8401 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     8461 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     8521 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     8581 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     8641 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     8701 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     8761 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     8821 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     8881 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     8941 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     9001 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     9061 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     9121 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     9181 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     9241 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     9301 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     9361 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     9421 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     9481 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa
     9541 attaatacga ctcactatag g
//