BCR/ABL P190 transgenic construct

LOCUS       BCR/ABL P190 transgenic construct               13290 bp ds-DNA     circular SYN 08-FEB-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    BCR/ABL P190 transgenic construct
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13290)
  AUTHORS   Heisterkamp N, Jenster G, ten Hoeve J, Zovich D, Pattengale PK, 
            Groffen J
  TITLE     Acute leukaemia in bcr/abl transgenic mice.
  JOURNAL   Nature. 1990 Mar 15;344(6263):251-3.
  PUBMED    2179728
REFERENCE   2  (bases 1 to 13290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 8, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13290
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(171..1031)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1782..1801
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1782..1800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1809..1916
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     1825..1841
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1826..1842
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1876..1892)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12208..12224)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(12261..12279)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12300..12316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12300..12316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12313..12335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12324..12340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12348..12378)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12393..12414
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12531..12548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12702..13290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12782..12801)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     1801 gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
     1861 tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg gagctctgca
     1921 ctccgcccga aaagtgcgct cggctctgcc aaggacgcgg ggcgcgtgac tatgcgtggg
     1981 ctggagcaac cgcctgctgg gtgcaaaccc tttgcgcccg gactcgtcca acgactataa
     2041 agagggcagg ctgtcctcta agcgtcacca cgacttcaac gtcctgagta ccttctcctc
     2101 acttactccg tagctccagc ttcaccagat cccccggccg cgccatggtg gacccggtgg
     2161 gcttcgcgga ggcgtggaag gcgcagttcc cggactcaga gcccccgcgc atggagctgc
     2221 gctcagtggg cgacatcgag caggagctgg agcgctgcaa ggcctccatt cggcgcctgg
     2281 agcaggaggt gaaccaggag cgcttccgca tgatctacct gcagacgttg ctggccaagg
     2341 aaaagaagag ctatgaccgg cagcgatggg gcttccggcg cgcggcgcag gcccccgacg
     2401 gcgcctccga gccccgagcg tccgcgtcgc gcccgcagcc agcgcccgcc gacggagccg
     2461 acccgccgcc cgccgaggag cccgaggccc ggcccgacgg cgagggttct ccgggtaagg
     2521 ccaggcccgg gaccgcccgc aggcccgggg cagccgcgtc gggggaacgg gacgaccggg
     2581 gaccccccgc cagcgtggcg gcgctcaggt ccaacttcga gcggatacgc aagggccatg
     2641 gccagcccgg ggcggacgcc gagaagccct tctacgtgaa cgtcgagttt caccacgagc
     2701 gcggcctggt gaaggtcaac gacaaagagg tgtcggaccg catcagctcc ctgggcagcc
     2761 aggccatgca gatggagcgc aaaaagtccc agcacggcgc gggctcgagc gtgggggatg
     2821 catccaggcc cccttaccgg ggacgctcct cggagagcag ctgcggcgtc gacggcgact
     2881 acgaggacgc cgagttgaac ccccgcttcc tgaaggacaa cctgatcgac gccaatggcg
     2941 gtagcaggcc cccttggccg cccctggagt accagcccta ccagagcatc tacgtcgggg
     3001 gcatgatgga aggggagggc aagggcccgc tcctgcgcag ccagagcacc tctgagcagg
     3061 agaagcgcct tacctggccc cgcaggtcct actccccccg gagttttgag gattgcggag
     3121 gcggctatac cccggactgc agctccaatg agaacctcac ctccagcgag gaggacttct
     3181 cctctggcca gtccagccgc gtgtccccaa gccccaccac ctaccgcatg ttccgggaca
     3241 aaagccgctc tccctcgcag aactcgcaac agtccttcga cagcagcagt ccccccacgc
     3301 cgcagtgcca taagcggcac cggcactgcc cggttgtcgt gtccgaggcc accatcgtgg
     3361 gcgtccgcaa gaccgggcag atctggccca acgatggcga gggcgccttc catggagacg
     3421 caggtgagtt cctcacgcca cgtgcgtggg cacacctgca cgggggagga aaaccataga
     3481 cgagtagggg atacaaaaca gaagttggga gggaggagtg gatagttttg agtgtatcag
     3541 gtgcacttgt ggttcacgcg gatcctgcac ccgaacaaac tccatcccct cctccttcct
     3601 gaatgcatac tgttagtgtt tggaatggaa ttgggttgag gatggtcttg tgagggatgg
     3661 gaggtggttt gttcctttgg ttctagagca atgtgtttgt gaagtacagc ttgtgagttg
     3721 aaggaagatg cttcaggcag taacctcctt gctatgtgct ctctccatgc cgggcagtga
     3781 ggatggcata aaagccggag tcatctctag cgcccacatc ggatgctgtt agcagggact
     3841 gatttatgtc ttgggcattc cccgccaccc cactgatcct gttctggagg ggttatatga
     3901 cacgtttgtg aaacctgaga tatgctagct gctcctaggc catagaagtg tcaggtttgc
     3961 ttgctaacca gcagtcctga acacaagtca cagcaggtga gaataggaat tagaaggaag
     4021 tgaggcgaca ggcagttgtg actcagtggg ccaccgaaaa tcaactgcat ggagatttta
     4081 tctgggggtg aagcagagcc acacccgttc tgatctgcct ggctttgatg ggaggcttca
     4141 cccatgatta ttcaccacca ggctcctcca tggtggtaac ccagccccat ttgctgaccc
     4201 tccagtgtgc tcctgctgcc tcatcttgcc aaggataagc acccacaggt gcttatttgg
     4261 ggctcccaat ctgtggtcag attctagcac taagattaaa actatgggat tgtacgccta
     4321 acttctgtgc tagatgcaaa taccatcagg cattttgggt catgggacta tgttttgaga
     4381 tccagacaca gtttgccatt ggggtaagga aagggtgggc tggggtgtgt gtgtgaggct
     4441 gtcgggaatc ttttggagtc ctggtccctg ttaagctcat ctggttttct gcttcattga
     4501 tttttctttt tctgctgatg gaggagttga atgtgcttgg tttgtcaaaa tcctcttcaa
     4561 acgagtgagg ctatttttgg ctcttagagc tgctgccttt ttgtggtggg cactctgtcc
     4621 tgtttgtgtt aggtacttcc agttactgat cttccatttg cagctggggt ttcctgtggt
     4681 gcacggctcc gacctgccgt ctggccgggc tgcattccct aagtccctat gtgggggcgg
     4741 ggtggaaggc tgtactattc agaagcctgc tttgcaggca catcagctcg ttcggggtct
     4801 ggaggattgc cttgtggtta ctgggcacca gccttgtctg gccagctgtt ggtcaggcga
     4861 gccagcttga actggccctc agtgccatgg tgcaggagag ttattaataa agaagaaaat
     4921 gagaggtttt tagggagatg tgcaaattga aattgatagt ctttgctggt ggagaattcc
     4981 tgcagcccgg gggatctact tgaggttatt tgatttgctg gtttgcaaga actcgtctac
     5041 acgttgtctt tatcacaaag aaattctgga gcatttgagc cctgttcaaa aaaaaaaggc
     5101 aagaagaaac ttcaggaaca aataacctat ttgaccctgt aatgaataat attcacacat
     5161 tttctaattt aagagctgtt gattgatttc caacttttag aatctgtttc agacaaaaca
     5221 tggaagttta gtaacagttt tttctattct gtaataataa ttgccacaag gttaaaaaaa
     5281 aaaatttaca gaagaacata tttgagggtg gtcgtggggg caagaaagct ggaaggacgg
     5341 gtggggtgtg ttaatagcct tgtcttgtga agtaagtcct cagagacgct gtcctagttg
     5401 gaagaacagg cagtgaaggt gtgcgtctgt tacggagaat aggactccag tgatctagct
     5461 ggtacttaga gaggtggggg tgatcaccaa aaaaacagct ctgcctctgg gagcggagag
     5521 gactgggata gaaagtattt gtctttcatt ttaagccctt ctgtacgatt tgacttttct
     5581 tttctacagg cacctgccac catgcccggc tgatttttgt atttgtagta aagacaggat
     5641 ttcactgtgt tggtcaggtt ggtcttgaac tcctgacctc atgatccacc cccggctttt
     5701 ttttttgaca cggagtttct cgttacccag gctggagtgc agtggcgcaa tctctgctca
     5761 ctgagacctc cacctcctgg gttcaagcga ttctcctgcc tcagcctcct gagtagctgg
     5821 gactacaggt gtgcgtcacc atgcccggct aatttttgta tgtctaatgg agacagggtt
     5881 tcaccatgtt ggtcaagttg gtctcaaact cctgaactca ggtgatccac caccttggcc
     5941 tcccaaagtg ctgggattac gggcgtgagc caccatgccc ggctaaagca agaaattttc
     6001 attgcatatt ttaagcaaag gcaaatgcat atgtggatag actgttttaa tttgactaaa
     6061 gtcatattga atccatgaat tttagaagct caaactattg gggaacaata attaccacct
     6121 tggagtgaaa atacttaatt tccacaagat ttagtaaagg aagagttttt taaaaaccac
     6181 cttaatgata atagtatgta cagatgttaa gaaatgaaat aggaatgtgt aatgttggaa
     6241 acacaaatat ttttgcttct gagaataaaa ctaatttttt ctcccaattt tctcttcctt
     6301 tttctttttt ctgttccccc ctttctcttc cagaagccct tcagcggcca gtagcatctg
     6361 actttgagcc tcagggtctg agtgaagccg ctcgttggaa ctccaaggaa aaccttctcg
     6421 ctggacccag tgaaaatgac cccaaccttt tcgttgcact gtatgatttt gtggccagtg
     6481 gagataacac tctaagcata actaaaggta aaagggttgt gggcagctag tggtggttgc
     6541 aggagataga aatctgggaa ttgcggtttg acctaccacc ctttgctcgt taaaggagca
     6601 gctttgaaat ctggactgca gggatatcca aaacaacaac tgcatgtttc taagggagtc
     6661 gactctcctt agaggagttc ttgtacaata gccctgggca aaaacagaac ttgccctatt
     6721 ttttatactg aaaaggacag ctggacaaaa tactgaacgc aatttttccc ctaagaaaaa
     6781 gcattatttc cctaaaatgt cttatattag gaacagagca cttgaataaa cataattgat
     6841 ttataaaaac tgaggctata cacttaccta tctgttcagt acaaacagga agcttcaaat
     6901 gtaaacgtga attctcatac acttataaat gcatatcttt atgtggactt gttaaaatga
     6961 aattggtatt taggaatttg gagattttta gtagttacac aagaatcaat gaaaaagaac
     7021 gaagctggtt tccaaagctg atatgtctga tttggttcct ttcttctcag gtgaaaagct
     7081 ccgggtctta ggctataatc acaatgggga atggtgtgaa gcccaaacca aaaatggcca
     7141 aggctgggtc ccaagcaact acatcacgcc agtcaacagt ctggagaaac actcctggta
     7201 ccatgggcct gtgtcccgca atgccgctga gtatctgctg agcagcggga tcaatggcag
     7261 cttcttggtg cgtgagagtg agagcagtcc tggccagagg tccatctcgc tgagatacga
     7321 agggagggtg taccattaca ggatcaacac tgcttctgat ggcaagctct acgtctcctc
     7381 cgagagccgc ttcaacaccc tggccgagtt ggttcatcat cattcaacgg tggccgacgg
     7441 gctcatcacc acgctccatt atccagcccc aaagcgcaac aagcccactg tctatggtgt
     7501 gtcccccaac tacgacaagt gggagatgga acgcacggac atcaccatga agcacaagct
     7561 gggcgggggc cagtacgggg aggtgtacga gggcgtgtgg aagaaataca gcctgacggt
     7621 ggccgtgaag accttgaagg aggacaccat ggaggtggaa gagttcttga aagaagctgc
     7681 agtcatgaaa gagatcaaac accctaacct ggtgcagctc cttggggtct gcacccggga
     7741 gcccccgttc tatatcatca ctgagttcat gacctacggg aacctcctgg actacctgag
     7801 ggagtgcaac cggcaggagg tgaacgccgt ggtgctgctg tacatggcca ctcagatctc
     7861 gtcagccatg gagtacctgg agaagaaaaa cttcatccac agagatcttg ctgcccgaaa
     7921 ctgcctggta ggggagaacc acttggtgaa ggtagctgat tttggcctga gcaggttgat
     7981 gacaggggac acctacacag cccatgctgg agccaagttc cccatcaaat ggactgcacc
     8041 cgagagcctg gcctacaaca agttctccat caagtccgac gtctgggcat ttggagtatt
     8101 gctttgggaa attgctacct atggcatgtc cccttacccg ggaattgacc tgtcccaggt
     8161 gtatgagctg ctagagaagg actaccgcat ggagcgccca gaaggctgcc cagagaaggt
     8221 ctatgaactc atgcgagcat gttggcagtg gaatccctct gaccggccct cctttgctga
     8281 aatccaccaa gcctttgaaa caatgttcca ggaatccagt atctcagacg aagtggaaaa
     8341 ggagctgggg aaacaaggcg tccgtggggc tgtgagtacc ttgctgcagg ccccagagct
     8401 gcccaccaag acgaggacct ccaggagagc tgcagagcac agagacacca ctgacgtgcc
     8461 tgagatgcct cactccaagg gccagggaga gagcgatcct ctggaccatg agcctgccgt
     8521 gtctccattg ctccctcgaa aagagcgagg tcccccggag ggcggcctga atgaagatga
     8581 gcgccttctc cccaaagaca aaaagaccaa cttgttcagc gccttgatca agaagaagaa
     8641 gaagacagcc ccaacccctc ccaaacgcag cagctccttc cgggagatgg acggccagcc
     8701 ggagcgcaga ggggccggcg aggaagaggg ccgagacatc agcaacgggg cactggcttt
     8761 cacccccttg gacacagctg acccagccaa gtccccaaag cccagcaatg gggctggggt
     8821 ccccaatgga gccctccggg agtccggggg ctcaggcttc cggtctcccc acctgtggaa
     8881 gaagtccagc acgctgacca gcagccgcct agccaccggc gaggaggagg gcggtggcag
     8941 ctccagcaag cgcttcctgc gctcttgctc cgcctcctgc gttccccatg gggccaagga
     9001 cacggagtgg aggtcagtca cgctgcctcg ggacttgcag tccacgggaa gacagtttga
     9061 ctcgtccaca tttggagggc acaaaagtga gaagccggct ctgcctcgga agagggcagg
     9121 ggagaacagg tctgaccagg tgacccgagg cacagtaacg cctcccccca ggctggtgaa
     9181 aaagaatgag gaagctgctg atgaggtctt caaagacatc atggagtcca gcccgggctc
     9241 cagcctgccc aacctgactc caaaacccct ccggcggcag gtcaccgtgg cccctgcctc
     9301 gggcctcccc cacaaggaag aagctggaaa gggcagtgcc ttagggaccc ctgctgcagc
     9361 tgagccagtg acccccacca gcaaagcagg ctcaggtgca ccagggggca ccagcaaggg
     9421 ccccgccgag gagtccagag tgaggaggca caagcactcc tctgagtcgc cagggaggga
     9481 caaggggaaa ttgtccaggc tcaaacctgc cccgccgccc ccaccagcag cctctgcagg
     9541 gaaggctgga ggaaagccct cgcagagccc gagccaggag gcggccgggg aggcagtcct
     9601 gggcgcaaag acaaaagcca cgagtctggt tgatgctgtg aacagtgacg ctgccaagcc
     9661 cagccagccg ggagagggcc tcaaaaagcc cgtgctcccg gccactccaa agccacagtc
     9721 cgccaagccg tcggggaccc ccatcagccc agcccccgtt ccctccacgt tgccatcagc
     9781 atcctcggcc ctggcagggg accagccgtc ttccaccgcc ttcatccctc tcatatcaac
     9841 ccgagtgtct cttcggaaaa cccgccagcc tccagagcgg atcgccagcg gcgccatcac
     9901 caagggcgtg gtcctggaca gcaccgaggc gctgtgcctc gccatctcta ggaactccga
     9961 gcagatggcc agccacagcg cagtgctgga ggccggcaaa aacctctaca cgttctgcgt
    10021 gagctatgtg gattccatcc agcaaatgag gaacaagttt gccttccgag aggccatcaa
    10081 caaactggag aataatctcc gggagcttca gatctgcccg gcgacagcag gcagtggtcc
    10141 agcggccact caggacttca gcaagctcct cagttcggtg aaggaaatca gtgacatagt
    10201 gcagaggtag cagcagtcag gggtcaggtg tcaggcccgt cggagctgcc tgcagcacat
    10261 gcgggctcgc ccatacccgt gacagtggct gacaagggac tagtgagtca gcaccttggc
    10321 ccaggagctc tgcgccaggc agagctgagg gccctgtgga gtccagctct actacctacg
    10381 tttgcaccgc ctgccctccc gcaccttcct cctccccgct ccgtctctgt cctcgaattt
    10441 tatctgtgga gttcctgctc cgtggactgc agtcggcatg ccaggacccg ccagccccgc
    10501 tcccacctag tgccccagac tgagctctcc aggccaggtg ggaacggctg atgtggactg
    10561 tctttttcat ttttttctct ctggagcccc tcctcccccg gctgggcctc cttcttccac
    10621 ttctccaaga atggaagcct gaactgaggc cttgtgtgtc aggccctctg cctgcactcc
    10681 ctggccttgc ccgtcgtgtg ctgaagacat gtttcaagaa ccgcatttcg ggaagggcat
    10741 gcacgggcat gcacacggct ggtcactctg ccctctgctg ctgcccgggg tggggtgcac
    10801 tcgccatttc ctcacgtgca ggacagctct tgatttgggt ggaaaacagg gtgctaaagc
    10861 caaccagcct ttgggtcctg ggcaggtggg agctgaaaag gatcgaggca tggggcatgt
    10921 cctttccatc tgtccacatc cccagagccc agctcttgct ctcttgtgac gtgcactgtg
    10981 aatcctggca agaaagcttg agtctcaagg gtggcaggtc actgtcactg ccgacatccc
    11041 tcccccagca gaatggaggc aggggacaag ggaggcagtg gctagtgggg tgaacagctg
    11101 gtgccaaata gccccagact gggcccaggc aggtctgcaa gggcccagag tgaaccgtcc
    11161 tttcacacat ctgggtgccc tgaaagggcc cttcccctcc cccactcctc taagacaaag
    11221 tagattctta caaggccctt tcctttggaa caagacagcc ttcacttttc tgagttcttg
    11281 aagcatttca aagccctgcc tctgtgtagc cgccctgaga gagaatagag ctgccactgg
    11341 gcacctgcgc acaggtggga ggaaagggcc tggccagtcc tggtcctggc tgcactcttg
    11401 aactgggcga atgtcttatt taattaccgt gagtgacata gcctcatgtt ctgtgggggt
    11461 catcagggag ggttaggaaa accacaaacg gagcccctga aagcctcacg tatttcacag
    11521 agcacgcctg ccatcttctc cccgaggctg ccccaggccg gagcccagat acgggggctg
    11581 tgactctggg cagggacccg gggtctcctg gaccttgaca gagcagctaa ctccgagagc
    11641 agtgggcagg tggccgcccc tgaggcttca cgccgggaga agccaccttc ccaccccttc
    11701 ataccgcctc gtgccagcag cctcgcacag gccctagctt tacgctcatc acctaaactt
    11761 gtactttatt tttctgatag aaatggtttc ctctggatcg ttttatgcgg ttcttacagc
    11821 acatcacctc tttgcccccg acggctgtga cgcagccgga gggaggcact agtcaccgac
    11881 agcggccttg aagacagagc aaagcgccca cccaggtccc ccgactgcct gtctccatga
    11941 ggtactggtc ccttcctttt gttaacgtga tgtgccacta tattttacac gtatctcttg
    12001 gtatgcatct tttatagacg ctcttttcta agtggcgtgt gcatagcgtc ctgccctgcc
    12061 ccctcggggg cctgtggtgg ctccccctct gcttctcggg gtccagtgca ttttgtttct
    12121 gtatatgatt ctctgtggtt ttttttgaat ccaaatctgt cctctgtagt attttttaaa
    12181 taaatccgga attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg
    12241 tggagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
    12301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    12361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    12421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    12481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    12541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    12601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    12661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    12721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    12781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    12841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    12901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    12961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    13021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    13081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    13141 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    13201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    13261 cagattacgc gcagaaaaaa aggatctcaa
//