Lipofectamine 3000传染试剂Invitrogen L3000008

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规格: 0.75mL 0.75mL*10

联系方式:I47-825O-882O

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Invitrogen Lipofectamine 2000传染试剂产品基本信息

产品名称: Invitrogen Lipofectamine 3000试剂、Invitrogen Lipofectamine 3000传染试剂
产品又名: Invitrogen Lipofectamine 3000试剂、Invitrogen Lipofectamine 3000传染试剂
英文名称: Invitrogen Lipofectamine 3000, Transfection Reagent
试剂类型: 传染试剂
级别: For Transfection
品牌: Invitrogen
产品目录号: L3000008、L3000-008
环保特征: 可循环使用包装
细胞类型: 已建立的细胞系,干细胞,原代细胞,难以转染的细胞
样品类型: 质粒DNA,合成siRNA,RNA干扰质粒(shRNA,miR)
转染技术: 脂质转染
产品规格: 24孔板
产品规格: 0.75mL
分类: 不含动物源
血清兼容性: Yes
保存条件: 2-8°C保存,不要冷冻

Invitrogen Lipofectamine 3000传染试剂产品描述:

Lipofectamine® 3000转染试剂中包含了我们最为先进的脂质体纳米颗粒技术,能够提供卓越的转染性能,改善相关应用的结果及可重复性。此试剂具有优异的转染效率,并可提高细胞活性,广泛适用于难转染的及常见的细胞种类(例如HEK293和Hela细胞)。
 
在对生物学研究相关的广泛细胞种类实施成功转染的过程中,本试剂可:
 
• 提供卓越的性能——我们针对难以转染的细胞种类实现了最高转染效率的试剂
• 提高细胞活性——对细胞作用温和,毒性很低
• 功能多样——同时适用于DNA、RNA及共转染应用


有效传染难于传染的细胞

Lipofectamine® 3000试剂专门针对难转染的细胞种类进行了优化,能够为广泛的细胞种类提供卓越的转染性能。其可高效转染的细胞类型包括:
成纤维细胞(3T3、COS-7) 成肌细胞(C2C12、L6 CRL-1458)
肝细胞(HepG2、HuH7) 红白血病细胞(K562)
乳腺癌(MCF7、Hs578T) 前列腺癌(LNCap)
肺癌细胞(A549、NCI-H460) 骨肉瘤细胞(U-2 OS、Saos-2)
结肠癌细胞(Caco2、SW480) 胰腺癌细胞(PANC-1)
皮肤黑色素瘤细胞(SK-MEL-28)

经济高效地传染常规细胞

对于常见的细胞种类而言,Lipofectamine® 3000试剂相比其他试剂具有更高的浓度和更低的用量,从而为客户带来更好的性价比。1.5 mL规格产品即足以完成最多1500次的转染反应(24孔板中)。

在低毒条件下获得优异的传染效率

Lipofectamine® 3000试剂的脂质体用量在很宽的动态范围内均能保持很高的转染效率,可按需进行简便快速的条件优化。在需要将细胞损伤降至最低的相关应用中,建议使用低毒的脂质体用量(24孔板每孔使用0.75 µL)。

在新型基因组编辑的相关应用中获得高传染效率

Lipofectamine® 3000试剂适用于新型基因组编辑的相关应用。通过高效率的转染,它能够增加TALEN或CRISPR系统剪切和重组的成功概率,最终使得基因修饰的效率实现最大化,并简化后续处理步骤。

Gibco细胞培养基选择指南

Transfection Reagent (细胞转染试剂)
产品描述 规格 货号
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培养基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培养基)
产品描述 规格 货号
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培养基)
产品描述 规格 货号
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基础培养基
产品描述 规格 货号
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 细胞减血清培养基
产品描述 规格 货号
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 细胞培养缓冲液
产品描述 规格 货号
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆虫培养基
产品描述 规格 货号
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 细胞培养试剂
产品描述 规格 货号
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青链霉素双抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(无菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

Invitrogen Lipofectamine 2000传染试剂产品说明书pdf版和相关资料下载

Invitrogen Lipofectamine 2000传染试剂产品应用举例

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