DMEM培养基Gibco 31053028

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规格: 500ml 500ml*10

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Gibco DMEM高糖不含谷氨酰胺无酚红培养基产品基本信息

产品名称: Gibco DMEM培养基、Gibco DMEM高糖不含谷氨酰胺无酚红培养基
产品又名: Gibco DMEM细胞培养基、Gibco DMEM高糖不含谷氨酰胺无酚红细胞培养基
英文名称: Gibco DMEM, high glucose, no glutamine, no phenol red
培养基类型: 哺乳细胞培养基
级别: For Mammalian Cell Culture
品牌: Gibco
产品目录号: 31053028、31053-028
葡萄糖: 高糖
葡萄糖浓度: 4500 mg/L
谷氨酰胺: 不含L-谷氨酰胺
酚红: 不含酚红
钠: 不含钠
HEPES 不含HEPES
环保特征: 可循环使用包装
产品规格: 500ml
血清添加: 使用时,正常浓度添加血清。
运输条件: 室温
产品外观: 透明液体
保存条件: 密封,2-8°C保存。
注意事项: 避免呼入和皮肤接触。
Gibco: 新鲜酵母浸出液
热灭活马血清

Gibco DMEM培养基图片

DMEM, high glucose, no glutamine, no phenol red

Gibco DMEM细胞培养基产品描述:

DMEM(Dulbecco's Modified Eagle Medium)是一种广泛应用的基础培养基,用于支持多种哺乳动物细胞的生长。在DMEM中成功培养的细胞包括原代成纤维细胞、神经元、胶质细胞、HUVECs和平滑肌细胞,以及HeLa、293、Cos-7和PC-12等细胞系。Life Technologies为一系列细胞培养应用提供了多种DMEM培养基。


Gibco DMEM培养基使用方法

与其他培养基相比,DMEM是独特的,因为它含有4倍的氨基酸和维生素的浓度比原来的EMEM培养基。DMEM最初是用低葡萄糖(1 g/L)和钠配制的,但通常与高葡萄糖水平一起使用,有或没有钠。DMEM不含蛋白质、脂类或生长因子。因此,DMEM需要补充,通常添加10%的胎牛血清(FBS)。DMEM使用碳酸氢钠缓冲系统(3.7 g/L),因此需要5–10%的CO2环境来维持生理pH值。 

Gibco DMEM培养基质量生产与质量体系

DMEM由位于纽约格兰德岛的cGMP兼容标准工厂生产。该设施在FDA注册为医疗器械制造商,并通过ISO 13485标准认证。为保持供应链的连续性,Life Technologies提供了在苏格兰工厂(41965-039)生产的相同DMEM产品。该工厂在FDA注册为医疗器械制造商,并通过ISO 13485标准认证。

Gibco DMEM培养基配方

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 30 0.4
L-Arginine hydrochloride 211 84 0.39810428
L-Cystine 2HCl 313 63 0.20127796
L-Histidine hydrochloride-H2O 210 42 0.2
L-Isoleucine 131 105 0.8015267
L-Leucine 131 105 0.8015267
L-Lysine hydrochloride 183 146 0.7978142
L-Methionine 149 30 0.20134228
L-Phenylalanine 165 66 0.4
L-Serine 105 42 0.4
L-Threonine 119 95 0.79831934
L-Tryptophan 204 16 0.078431375
L-Tyrosine disodium salt dihydrate 261 104 0.39846742
L-Valine 117 94 0.8034188
Vitamins
Choline chloride 140 4 0.028571429
D-Calcium pantothenate 477 4 0.008385744
Folic Acid 441 4 0.009070295
Niacinamide 122 4 0.032786883
Pyridoxine hydrochloride 206 4 0.019417476
Riboflavin 376 0.4 0.00106383
Thiamine hydrochloride 337 4 0.011869436
i-Inositol 180 7.2 0.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 200 1.8018018
Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.1 2.48E-04
Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.8139166
Potassium Chloride (KCl) 75 400 5.3333335
Sodium Bicarbonate (NaHCO3) 84 3700 44.04762
Sodium Chloride (NaCl) 58 6400 110.344826
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 125 0.9057971
Other Components
D-Glucose (Dextrose) 180 4500 25


Gibco细胞培养基选择指南

Transfection Reagent (细胞转染试剂)
产品描述 规格 货号
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培养基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培养基)
产品描述 规格 货号
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培养基)
产品描述 规格 货号
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基础培养基
产品描述 规格 货号
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 细胞减血清培养基
产品描述 规格 货号
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 细胞培养缓冲液
产品描述 规格 货号
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆虫培养基
产品描述 规格 货号
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 细胞培养试剂
产品描述 规格 货号
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青链霉素双抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(无菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

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