| 产品名称： | Gibco Advanced DMEM培养基、Gibco Advanced DMEM高糖培养基 |
|---|---|
| 产品又名： | Gibco Advanced DMEM细胞培养基、Gibco Advanced DMEM细胞培养基 |
| 英文名称： | Gibco Advanced DMEM |
| 培养基类型： | 哺乳细胞培养基 |
| 级别： | For Mammalian Cell Culture |
| 品牌： | Gibco |
| 产品目录号： | 12491015，12491-015 |
| 葡萄糖: | 高糖 |
| 谷氨酰胺： | 不含L-谷氨酰胺 |
| 酚红： | 含酚红 |
| 丙酮酸钠： | 含丙酮酸钠 |
| HEPES | 不含HEPES |
| 环保特征: | 可循环使用包装 |
| 产品规格： | 500ml |
| 血清添加: | 使用时，正常浓度添加血清。 |
| 运输条件： | 室温 |
| 产品外观： | 红色液体 |
| 颜色与澄清度： | 红色透明至微不透明液体。 |
| 保存条件： | 密封，2-8°C保存。 |
| 注意事项： | 避免呼入和皮肤接触。 |
| Gibco： |
新鲜酵母浸出液 热灭活马血清 |
Gibco Advanced DMEM细胞培养基产品描述：
高级DMEM（Dulbecco改良的Eagle培养基）是一种广泛使用的基础培养基，可用于哺乳动物细胞的培养。与传统的DMEM相比，血清补充剂可以减少50-90%，而生长速度和形态没有变化。在晚期DMEM中成功培养的细胞没有适应性，包括MDBK、HepG2、COS-7、A549、MDCK、WI-38和Vero。Gibco Advanced DMEM培养基使用方法
Advanced DMEM与其他培养基不同，因为添加了以下成分以减少血清：乙醇胺、谷胱甘肽、抗坏血酸、胰岛素、转铁蛋白、AlbuMAX®I细胞培养用富含脂质的牛血清白蛋白，以及微量元素亚硒酸钠、偏钒酸铵、硫酸铜和氯化锰。晚期DMEM需要补充1-5%胎牛血清和4 mM L-谷氨酰胺或谷氨酰胺™ 补充。许多细胞系不需要适应这种介质。必须为每个细胞系优化FBS浓度，以获得最大的血清还原率。高级DMEM使用碳酸氢钠缓冲系统（3.7 g/L），因此需要5–10%的CO2环境来维持生理pH值。cGMP生产与质量体系
为了供应链的连续性，我们在位于纽约州格兰德岛和英国苏格兰的两个独立工厂生产先进的DMEM。这两个工厂都符合cGMP生产要求，通过了ISO 13485认证，并在FDA注册为医疗器械制造商。Gibco Advanced DMEM培养基配方
| Components | Molecular Weight | Concentration (mg/L) | mM |
| Amino Acids | |||
| Glycine | 75 | 37.5 | 0.5 |
| L-Alanine | 8.9 | Infinity | |
| L-Arginine hydrochloride | 84 | Infinity | |
| L-Asparagine | 13.2 | Infinity | |
| L-Aspartic acid | 13.3 | Infinity | |
| L-Cystine 2HCl | 63 | Infinity | |
| L-Glutamic Acid | 14.7 | Infinity | |
| L-Histidine hydrochloride-H2O | 42 | Infinity | |
| L-Isoleucine | 105 | Infinity | |
| L-Leucine | 105 | Infinity | |
| L-Lysine hydrochloride | 146 | Infinity | |
| L-Methionine | 30 | Infinity | |
| L-Phenylalanine | 66 | Infinity | |
| L-Proline | 11.5 | Infinity | |
| L-Serine | 52.5 | Infinity | |
| L-Threonine | 95 | Infinity | |
| L-Tryptophan | 16 | Infinity | |
| L-Tyrosine disodium salt dihydrate | 104 | Infinity | |
| L-Valine | 94 | Infinity | |
| Vitamins | |||
| Ascorbic Acid phosphate | 2.5 | Infinity | |
| Choline chloride | 4 | Infinity | |
| D-Calcium pantothenate | 477 | 4 | 0.008385744 |
| Folic Acid | 441 | 4 | 0.009070295 |
| Niacinamide | 4 | Infinity | |
| Pyridoxine hydrochloride | 4 | Infinity | |
| Riboflavin | 0.4 | Infinity | |
| Thiamine hydrochloride | 4 | Infinity | |
| i-Inositol | 7.2 | Infinity | |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8018018 |
| Ferric Nitrate (Fe(NO3)3"9H2O) | 0.1 | Infinity | |
| Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | Infinity | |
| Potassium Chloride (KCl) | 400 | Infinity | |
| Sodium Bicarbonate (NaHCO3) | 3700 | Infinity | |
| Sodium Chloride (NaCl) | 6400 | Infinity | |
| Sodium Phosphate dibasic (Na2HPO4-H2O) | 125 | Infinity | |
| Proteins | |||
| AlbuMAX® II | 400 | Infinity | |
| Human Transferrin (Holo) | 7.5 | Infinity | |
| Insulin Recombinant Full Chain | 10 | Infinity | |
| Trace Elements | |||
| Ammonium Metavanadate | 3.00E-04 | Infinity | |
| Cupric Sulfate | 0.00125 | Infinity | |
| Manganous Chloride | 5.00E-05 | Infinity | |
| Sodium Selenite | 0.005 | Infinity | |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 4500 | 25 |
| Ethanolamine | 1.9 | Infinity | |
| Glutathione (reduced) | 307 | 1 | 0.003257329 |
| Phenol Red | 15 | Infinity | |
| Sodium Pyruvate | 110 | Infinity | |
Gibco细胞培养基选择指南
| Transfection Reagent （细胞转染试剂） | ||
|---|---|---|
| 产品描述 | 规格 | 货号 |
|
Lipofectamin 2000 （Lipo2000、Lipo 2000） Each unit contains one vial of Lipofectamine 2000 Reagent. |
0.75 ml |
11668-027 11668027 |
|
Lipofectamin 3000 （Lipo3000、lipo 3000） Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent. |
0.75 ml |
L3000-008 L3000008 |
| Dulbecco’s Modified Eagle Media （DMEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose) Contains no L-glutamine or sodium pyruvate |
500 mL |
11960-044 11960044 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
|
500 mL |
11965-092 11965092 |
|
DMEM (1X), liquid (high glucose) (DMEM高糖培养基，最常用)
Contains L-glutamine and sodium pyruvate
|
500 mL |
11995-065 11995065 |
|
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
|
500 mL |
10313-021 10313021 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
|
500 mL |
21063-029 21063029 |
|
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
|
500 mL |
31053-028 31053028 |
|
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
|
500 mL |
11885-084 11885084 |
|
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
|
500 mL |
12430-054 12430054 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
|
500 mL |
11039-021 11039021 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
|
500 mL |
11320-033 11320033 |
|
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
|
500 mL |
11330-032 11330032 |
| RPMI Media （RPMI1640培养基） | ||
| 产品描述 | 规格 | 货号 |
|
RPMI 1640 (1X), liquid（最常用）
Contains L-glutamine
|
500 mL |
11875-093 11875093 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
|
500 mL |
11835-030 11835030 |
|
RPMI 1640 (1X), liquid
Contains no L-glutamine
|
500 mL |
21870-076 21870076 |
|
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
|
500 mL |
22400-089 22400089 |
| Minimum Essential Media （MEM培养基） | ||
| 产品描述 | 规格 | 货号 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
|
500 mL |
11095-080 11095080 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
|
500 mL |
10370-021 10370021 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
|
500 mL |
11090-081 11090081 |
|
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
|
500 mL |
12571-063 12571063 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
|
500 mL |
12561-056 12561056 |
|
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
|
500 mL |
41061-029 41061029 |
| GlutaMAX™ Media （GlutaMAX系列培养基） | ||
| 产品描述 | 规格 | 货号 |
|
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10569-010 10569010 |
|
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
|
500 mL |
10564-011 10564011 |
|
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
|
500 mL |
10566-016 10566016 |
|
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
|
500 mL |
10567-014 10567014 |
|
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
|
500 mL |
10565-018 10565018 |
| F12 Nutrient Mixture (1X), liquid | 500 mL |
31765-035 31765035 |
|
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
|
500 mL |
31980-030 31980-030 |
|
MEM α (1X), liquid （最常用）
Contains no ribonucleosides or deoxyribonucleosides
|
500 mL |
32561-037 32561037 |
|
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
|
500 mL |
32571-036 32571036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts
|
500 mL |
41090-036 41090036 |
|
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
|
500 mL |
42360-032 42360032 |
| OptiMEM® I Reduced Serum Medium (1X), liquid | 500 mL |
51985-034 51985034 |
| RPMI 1640 (1X), liquid | 500 mL |
61870-036 61870036 |
|
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
|
500 mL |
72400-047 72400047 |
| 其他常用基础培养基 | ||
| 产品描述 | 规格 | 货号 |
|
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
|
500 mL |
11765-054 11765054 |
|
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
|
500 mL |
21127-022 21127022 |
|
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
|
12440-053 12440053 |
|
|
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
|
11415-064 11415064 |
|
|
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
|
16600-082 16600082 |
|
|
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
|
11150-059 11150059 |
|
| Reduced Serum Media 细胞减血清培养基 | ||
| 产品描述 | 规格 | 货号 |
| Advanced D-MEM (1X), liquid | 500 mL |
12491-015 12491015 |
| Advanced D-MEM/F12 (1X), liquid | 500 mL |
12634-010 12634010 |
| Advanced MEM (1X), liquid | 500 mL |
12492-013 12492013 |
| Advanced RPMI 1640 (1X), liquid | 500 mL |
12633-012 12633012 |
| Opti-MEM®-I Reduced Serum Medium (1X), liquid | 500 mL |
31985-070 31985070 |
| Balanced Salt Solutions 细胞培养缓冲液 | ||
| 产品描述 | 规格 | 货号 |
|
DPBS (1X), liquid
Contains calcium and magnesium
|
500 mL |
14040-133 14040133 |
|
DPBS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14190-144 14190144 |
|
DPBS (10X), liquid
Contains no calcium or magnesium
|
500 mL |
14200-075 14200075 |
|
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
|
500 mL |
14025-092 14025092 |
|
HBSS (1X), liquid
Contains no calcium or magnesium
|
500 mL |
14170-112 14170112 |
|
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
|
500 mL |
14175-095 14175-095 |
| PBS 7.4 (1X), liquid | 500 mL |
10010-023 10010023 |
| PBS 7.4 (10X), liquid | 500 mL |
70011-044 70011044 |
| PBS 7.2 (1X), liquid | 500 mL |
20012-027 20012027 |
| PBS 7.2 (10X), liquid | 500 mL |
70013-032 70013032 |
| Insect Media 昆虫培养基 | ||
| 产品描述 | 规格 | 货号 |
|
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
|
500 mL |
11605-094 11605094 |
|
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
|
500 mL |
11720-034 11720034 |
|
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
|
1000 mL |
10902-088 10902088 |
| Reagents 细胞培养试剂 | ||
| 产品描述 | 规格 | 货号 |
|
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12605-010 12605010 |
|
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
|
100 mL |
12604-013 12604013 |
|
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid（胰蛋白酶，最常用）
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
|
100 mL |
25200-056 25200056 |
|
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red |
100 mL |
25300-054 25300054 |
|
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
|
100 mL |
15630-080 15630080 |
| L-glutamine-200 mM (100X), liquid | 100 mL |
25030-081 25030081 |
| GlutaMAX™-I (100X), liquid | 100 mL |
35050-061 35050061 |
|
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
|
100 mL |
15240-062 15240062 |
|
Penicillin-Streptomycin, liquid （青链霉素双抗）
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
|
100 mL |
15140-122 15140122 |
| MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid | 100 mL |
11140-050 11140050 |
|
Distilled Water（无菌水）
Membrane filtered and endotoxin screened
|
500 mL |
15230-162 15230162 |
