| 保藏机构: | JCM |
|---|---|
| 菌种名称: | JCM4926, JCM 4926 |
| 英文名称: | Bradyrhizobium japonicum |
| 中文名称 | 慢生型大豆根瘤菌 |
| 存储人: | (Kirchner) Jordan |
| 来源历史: | |
| 其他保藏中心编号: | JCM 20679; DSM 30131; USDA 6; USDA 505; NBRC 14783 |
| 分离来源: | 大豆 |
| 菌种特性 | |
| 备注： | |
| 培养条件： | 28°C |
| 生物安全等级: | 1 |
| 氧气需求: | |
| 培养基: |
TSB培养基：513
R2A MEDIUM
Bacto Protesost Peptone No. 3 0.5 g
Bacto Casamino Acids 0.5 g
Bacto Dextrose 0.5 g
Soluble Starch 0.5 g
Potassium phosphate, Didbasic 0.3 g
Bacto Yeast Extract 0.5 g
Magnesium sulfate 0.05 g
Bacto Agar 15.0 g
Distilled water 1.0 L
Sodium Pyruvate 0.3 g
Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.
877
MANNITOL-YEAST EXTRACT MEDIUM
Mannitol 10g
Yeast extract 1g
K2HPO4 0.5g
MgSO4·7H2O 0.2g
NaCl 0.1g
Distilled water 1L
Agar 15g
Adjust pH to6.8 with HCl.
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| 模式菌株: | 是 |
| 参考文献: |
B2264
Jordan, D.C. 1982. Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants Int. J. Syst. Bacteriol. 32 : 136–139.
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