pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

LOCUS       pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA Sequencing Result	7446 bp 	DNA	SYN
DEFINITION  pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA Sequencing Result"
                     /mol_type="other DNA"
     promoter        155..731
                     /label="CMV_immearly_promoter"
     misc_feature    234..521
                     /label="CAG_enhancer"
     misc_feature    688..708
                     /label="CMV_fwd_primer"
     CDS             693..4106
                     /label="ORF frame 3"
     CDS             complement(744..3479)
                     /label="ORF frame 2"
     misc_feature    768..788
                     /label="SV40 NLS"
     misc_feature    768..791
                     /label="SV40 NLS"
     misc_feature    774..788
                     /label="NLS"
     CDS             complement(1973..2431)
                     /label="ORF frame 3"
     misc_feature    4023..4049
                     /label="HA"
     misc_feature    complement(4131..4148)
                     /label="BGH_rev_primer"
     terminator      4134..4337
                     /label="bGH_PA_terminator"
     promoter        4360..4592
                     /label="hU6_promoter"
     misc_feature    4522..4541
                     /label="LKO1_5_primer"
     rep_origin      4941..5247
                     /label="f1_origin"
     misc_feature    5515..5537
                     /label="pGEX_3_primer"
     promoter        5696..5724
                     /label="AmpR_promoter"
     gene            5766..6626
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             5766..6626
                     /label="ORF frame 3"
     rep_origin      6781..7400
                     /label="pBR322_origin"
ORIGIN
    1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT
   61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
  121 AGGGGTTCCT GCGGCCTCTA GACTCGAGGC GTTGACATTG ATTATTGACT AGTTATTAAT
  181 AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC
  241 TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA
  301 TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT
  361 ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC
  421 CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT
  481 GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC
  541 GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC
  601 TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA
  661 AATGTCGTAA CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG
  721 TCTATATAAG CAGAGCTCTC TGGCTAACTA CCGGTGCCAC CATGGCCCCA AAGAAGAAGC
  781 GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCAAGCGGAA CTACATCCTG GGCCTGGACA
  841 TCGGCATCAC CAGCGTGGGC TACGGCATCA TCGACTACGA GACACGGGAC GTGATCGATG
  901 CCGGCGTGCG GCTGTTCAAA GAGGCCAACG TGGAAAACAA CGAGGGCAGG CGGAGCAAGA
  961 GAGGCGCCAG AAGGCTGAAG CGGCGGAGGC GGCATAGAAT CCAGAGAGTG AAGAAGCTGC
 1021 TGTTCGACTA CAACCTGCTG ACCGACCACA GCGAGCTGAG CGGCATCAAC CCCTACGAGG
 1081 CCAGAGTGAA GGGCCTGAGC CAGAAGCTGA GCGAGGAAGA GTTCTCTGCC GCCCTGCTGC
 1141 ACCTGGCCAA GAGAAGAGGC GTGCACAACG TGAACGAGGT GGAAGAGGAC ACCGGCAACG
 1201 AGCTGTCCAC CAAAGAGCAG ATCAGCCGGA ACAGCAAGGC CCTGGAAGAG AAATACGTGG
 1261 CCGAACTGCA GCTGGAACGG CTGAAGAAAG ACGGCGAAGT GCGGGGCAGC ATCAACAGAT
 1321 TCAAGACCAG CGACTACGTG AAAGAAGCCA AACAGCTGCT GAAGGTGCAG AAGGCCTACC
 1381 ACCAGCTGGA CCAGAGCTTC ATCGACACCT ACATCGACCT GCTGGAAACC CGGCGGACCT
 1441 ACTATGAGGG ACCTGGCGAG GGCAGCCCCT TCGGCTGGAA GGACATCAAA GAATGGTACG
 1501 AGATGCTGAT GGGCCACTGC ACCTACTTCC CCGAGGAACT GCGGAGCGTG AAGTACGCCT
 1561 ACAACGCCGA CCTGTACAAC GCCCTGAACG ACCTGAACAA TCTCGTGATC ACCAGGGACG
 1621 AGAACGAGAA GCTGGAATAT TACGAGAAGT TCCAGATCAT CGAGAACGTG TTCAAGCAGA
 1681 AGAAGAAGCC CACCCTGAAG CAGATCGCCA AAGAAATCCT CGTGAACGAA GAGGATATTA
 1741 AGGGCTACAG AGTGACCAGC ACCGGCAAGC CCGAGTTCAC CAACCTGAAG GTGTACCACG
 1801 ACATCAAGGA CATTACCGCC CGGAAAGAGA TTATTGAGAA CGCCGAGCTG CTGGATCAGA
 1861 TTGCCAAGAT CCTGACCATC TACCAGAGCA GCGAGGACAT CCAGGAAGAA CTGACCAATC
 1921 TGAACTCCGA GCTGACCCAG GAAGAGATCG AGCAGATCTC TAATCTGAAG GGCTATACCG
 1981 GCACCCACAA CCTGAGCCTG AAGGCCATCA ACCTGATCCT GGACGAGCTG TGGCACACCA
 2041 ACGACAACCA GATCGCTATC TTCAACCGGC TGAAGCTGGT GCCCAAGAAG GTGGACCTGT
 2101 CCCAGCAGAA AGAGATCCCC ACCACCCTGG TGGACGACTT CATCCTGAGC CCCGTCGTGA
 2161 AGAGAAGCTT CATCCAGAGC ATCAAAGTGA TCAACGCCAT CATCAAGAAG TACGGCCTGC
 2221 CCAACGACAT CATTATCGAG CTGGCCCGCG AGAAGAACTC CAAGGACGCC CAGAAAATGA
 2281 TCAACGAGAT GCAGAAGCGG AACCGGCAGA CCAACGAGCG GATCGAGGAA ATCATCCGGA
 2341 CCACCGGCAA AGAGAACGCC AAGTACCTGA TCGAGAAGAT CAAGCTGCAC GACATGCAGG
 2401 AAGGCAAGTG CCTGTACAGC CTGGAAGCCA TCCCTCTGGA AGATCTGCTG AACAACCCCT
 2461 TCAACTATGA GGTGGACCAC ATCATCCCCA GAAGCGTGTC CTTCGACAAC AGCTTCAACA
 2521 ACAAGGTGCT CGTGAAGCAG GAAGAAAACA GCAAGAAGGG CAACCGGACC CCATTCCAGT
 2581 ACCTGAGCAG CAGCGACAGC AAGATCAGCT ACGAAACCTT CAAGAAGCAC ATCCTGAATC
 2641 TGGCCAAGGG CAAGGGCAGA ATCAGCAAGA CCAAGAAAGA GTATCTGCTG GAAGAACGGG
 2701 ACATCAACAG GTTCTCCGTG CAGAAAGACT TCATCAACCG GAACCTGGTG GATACCAGAT
 2761 ACGCCACCAG AGGCCTGATG AACCTGCTGC GGAGCTACTT CAGAGTGAAC AACCTGGACG
 2821 TGAAAGTGAA GTCCATCAAT GGCGGCTTCA CCAGCTTTCT GCGGCGGAAG TGGAAGTTTA
 2881 AGAAAGAGCG GAACAAGGGG TACAAGCACC ACGCCGAGGA CGCCCTGATC ATTGCCAACG
 2941 CCGATTTCAT CTTCAAAGAG TGGAAGAAAC TGGACAAGGC CAAAAAAGTG ATGGAAAACC
 3001 AGATGTTCGA GGAAAAGCAG GCCGAGAGCA TGCCCGAGAT CGAAACCGAG CAGGAGTACA
 3061 AAGAGATCTT CATCACCCCC CACCAGATCA AGCACATTAA GGACTTCAAG GACTACAAGT
 3121 ACAGCCACCG GGTGGACAAG AAGCCTAATA GAGAGCTGAT TAACGACACC CTGTACTCCA
 3181 CCCGGAAGGA CGACAAGGGC AACACCCTGA TCGTGAACAA TCTGAACGGC CTGTACGACA
 3241 AGGACAATGA CAAGCTGAAA AAGCTGATCA ACAAGAGCCC CGAAAAGCTG CTGATGTACC
 3301 ACCACGACCC CCAGACCTAC CAGAAACTGA AGCTGATTAT GGAACAGTAC GGCGACGAGA
 3361 AGAATCCCCT GTACAAGTAC TACGAGGAAA CCGGGAACTA CCTGACCAAG TACTCCAAAA
 3421 AGGACAACGG CCCCGTGATC AAGAAGATTA AGTATTACGG CAACAAACTG AACGCCCATC
 3481 TGGACATCAC CGACGACTAC CCCAACAGCA GAAACAAGGT CGTGAAGCTG TCCCTGAAGC
 3541 CCTACAGATT CGACGTGTAC CTGGACAATG GCGTGTACAA GTTCGTGACC GTGAAGAATC
 3601 TGGATGTGAT CAAAAAAGAA AACTACTACG AAGTGAATAG CAAGTGCTAT GAGGAAGCTA
 3661 AGAAGCTGAA GAAGATCAGC AACCAGGCCG AGTTTATCGC CTCCTTCTAC AACAACGATC
 3721 TGATCAAGAT CAACGGCGAG CTGTATAGAG TGATCGGCGT GAACAACGAC CTGCTGAACC
 3781 GGATCGAAGT GAACATGATC GACATCACCT ACCGCGAGTA CCTGGAAAAC ATGAACGACA
 3841 AGAGGCCCCC CAGGATCATT AAGACAATCG CCTCCAAGAC CCAGAGCATT AAGAAGTACA
 3901 GCACAGACAT TCTGGGCAAC CTGTATGAAG TGAAATCTAA GAAGCACCCT CAGATCATCA
 3961 AAAAGGGCAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG AAAAAGGGAT
 4021 CCTACCCATA CGATGTTCCA GATTACGCTT ACCCATACGA TGTTCCAGAT TACGCTTACC
 4081 CATACGATGT TCCAGATTAC GCTTAAGAAT TCCTAGAGCT CGCTGATCAG CCTCGACTGT
 4141 GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA
 4201 AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG
 4261 TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA
 4321 AGAGAATAGC AGGCATGCTG GGGAGGTACC GAGGGCCTAT TTCCCATGAT TCCTTCATAT
 4381 TTGCATATAC GATACAAGGC TGTTAGAGAG ATAATTGGAA TTAATTTGAC TGTAAACACA
 4441 AAGATATTAG TACAAAATAC GTGACGTAGA AAGTAATAAT TTCTTGGGTA GTTTGCAGTT
 4501 TTAAAATTAT GTTTTAAAAT GGACTATCAT ATGCTTACCG TAACTTGAAA GTATTTCGAT
 4561 TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACACCG GAGACCACGG CAGGTCTCAG
 4621 TTTTAGTACT CTGGAAACAG AATCTACTAA AACAAGGCAA AATGCCGTGT TTATCTCGTC
 4681 AACTTGTTGG CGAGATTTTT GCGGCCGCAG GAACCCCTAG TGATGGAGTT GGCCACTCCC
 4741 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC
 4801 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG
 4861 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT
 4921 AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
 4981 CCGCTACACT TGCCAGCGCC TTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
 5041 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
 5101 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG
 5161 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
 5221 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACTCTAT CTCGGGCTAT TCTTTTGATT
 5281 TATAAGGGAT TTTGCCGATT TCGGTCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
 5341 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA
 5401 TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC
 5461 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTccggga
 5521 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagACGA AAGGGCCTCG
 5581 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
 5641 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
 5701 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
 5761 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
 5821 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
 5881 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
 5941 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
 6001 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
 6061 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
 6121 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
 6181 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC
 6241 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA
 6301 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC
 6361 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
 6421 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG
 6481 GAAGCCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA
 6541 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG
 6601 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
 6661 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
 6721 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
 6781 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
 6841 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
 6901 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
 6961 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
 7021 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
 7081 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
 7141 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
 7201 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
 7261 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
 7321 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
 7381 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
 7441 ACATGT
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