pBiFC-VN155(I152L)

LOCUS       pBiFC-VN155(I152L)	4289 bp 	DNA	SYN
DEFINITION  pBiFC-VN155(I152L)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="pBiFC-VN155(I152L)"
                     /mol_type="other DNA"
     CDS             1..588
                     /label="ORF frame 1"
     misc_feature    4..27
                     /label="Myc_primer"
     gene            124..567
                     /label="EGFP (0 - 443)"
                     /gene="EGFP (0 - 443)"
     misc_feature    complement(166..187)
                     /label="EGFP_N_primer"
     terminator      complement(610..729)
                     /label="SV40_PA_terminator"
     misc_feature    complement(624..643)
                     /label="EBV_rev_primer"
     promoter        complement(841..859)
                     /label="M13_reverse_primer"
     misc_feature    complement(858..880)
                     /label="M13_pUC_rev_primer"
     promoter        complement(894..923)
                     /label="lac_promoter"
     gene            complement(2006..2866)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2006..2866)
                     /label="ORF frame 2"
     promoter        complement(2908..2936)
                     /label="AmpR_promoter"
     misc_feature    complement(3095..3117)
                     /label="pGEX_3_primer"
     misc_feature    complement(3316..3459)
                     /label="lacZ_a"
     misc_feature    3430..3452
                     /label="M13_pUC_fwd_primer"
     promoter        3445..3461
                     /label="M13_forward20_primer"
     misc_feature    3492..3779
                     /label="CAG_enhancer"
     promoter        3497..3989
                     /label="CMV_immearly_promoter"
     misc_feature    3946..3966
                     /label="CMV_fwd_primer"
     promoter        3947..4016
                     /label="CMV_promoter"
     promoter        3959..4078
                     /label="CMV2_promoter"
     misc_feature    3990..4009
                     /label="pCEP_fwd_primer"
     misc_feature    3992..4016
                     /label="LNCX_primer"
     misc_feature    4092..4118
                     /label="pCMV_primer"
     misc_feature    4133..4229
                     /label="modSV40_late_16s_int"
     misc_feature    4133..4163
                     /label="SV40_late_19s_int"
     CDS             4211..659
                     /label="ORF frame 2"
ORIGIN
    1 ATGGCATCAA TGCAGAAGCT GATCTCAGAG GAGGACCTGC TTATGGCCAT GGAGGCCCGA
   61 ATTCGGTCGA CCGAGATCTC TCGAGGTACC GGAGGTGGCG GGAGCGGAGG TGGCGGGAGT
  121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
  181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC
  241 GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
  301 CTCGTGACCA CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG
  361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
  421 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
  481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
  541 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATCTCA CCGCCTGAGC GGCCGCGGGG
  601 ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA
  661 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT
  721 GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGG
  781 TGTGGGAGGT TTTTTCGGAT CCTCTAGAGT CGATCTGCAG GCATGCTAGC TTGGCGTAAT
  841 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
  901 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA
  961 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
 1021 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC
 1081 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
 1141 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG
 1201 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC
 1261 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
 1321 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA
 1381 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
 1441 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG
 1501 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT
 1561 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
 1621 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA
 1681 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
 1741 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA
 1801 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG
 1861 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
 1921 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA
 1981 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
 2041 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA
 2101 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC
 2161 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
 2221 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA
 2281 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC
 2341 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT
 2401 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA
 2461 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
 2521 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG
 2581 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC
 2641 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT
 2701 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT
 2761 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
 2821 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC
 2881 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA
 2941 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG
 3001 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT
 3061 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
 3121 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG
 3181 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA
 3241 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA
 3301 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT
 3361 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC
 3421 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGTTCGAG CTTGCATGCC
 3481 TGCAGGTCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
 3541 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
 3601 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
 3661 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
 3721 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
 3781 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT
 3841 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA
 3901 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA
 3961 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCGTTT AGTGAACCGT CAGATCGCCT
 4021 GGAGACGCCA TCCACGCTGT TTTGACCTCC ATAGAAGACA CCGGGACCGA TCCAGCCTCC
 4081 GGACTCTAGA GGATCCGGTA CTAGAGGAAC TGAAAAACCA GAAAGTTAAC TGGTAAGTTT
 4141 AGTCTTTTTG TCTTTTATTT CAGGTCCCGG ATCCGGTGGT GGTGCAAATC AAAGAACTGC
 4201 TCCTCAGTGG ATGTTGCCTT TACTTCTAGG CCTGTACGGA AGTGTTACTT CTGCTCTAAA
 4261 AGCTGCGGAA TTGTACCCGC GGGCCCACC
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