JCM16185

价格:3200元

代购价格:询价

联系方式:I47-825O-882O

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JCM16185标准菌株基本信息

保藏机构: JCM
菌种名称: JCM4926, JCM 4926
英文名称: Desulfosporosinus acidiphilus
中文名称 嗜酸脱硫螺菌
存储人: Alazard et a/l.
来源历史: << DSMZ << D. Alazard, Lab. Microbiol. de l'IRD, Univ. de Provence et de la Mediterranee, Marseille, France; SJ4
其他保藏中心编号: DSM 22704; JCM 16185
分离来源: 酸性采矿废水倾析池沉积物
菌种特性  
备注:  
培养条件: 30°C
生物安全等级: 1
氧气需求: 需氧
培养基: TSB培养基:990
Desulfosporosinus Acidophilus Medium
Ca(NO3)2 x 5 H2O 0.02 g
D-Fructose 3.50 g
Distilled water 1000.00 mL
KCl 0.05 g
KH2PO4 10.00 g
L-Cysteine-HCl x H2O 0.50 g
MgSO4 x 7 H2O 0.50 g
(NH4)2SO4 0.45 g
Na2HPO4 1.00 g
Selenite-tungstate solution (see below) 1.00 mL
Trace element solution SL-10 (see below) 1.00 mL
Vitamin solution (see below) 10.00 mL
Yeast extract 0.50 g
Na-resazurin solution (0.1% w/v) 0.50 mL
Dissolve ingredients (except phosphates, vitamins, fructose and cysteine) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prepare a phosphate buffer stock solution with KH2PO4 (25% w/v) and Na2HPO4, adjust pH of the buffer to 5.8. Prior to inoculation add phosphate buffer, vitamins, fructose and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins and D-fructose should be sterilized by filtration. The pH of the complete medium should be at 5.8.
Selenite-tungstate solution
Distilled water 1000.0 mL
Na2SeO3 x 5 H2O 3.0 mg
NaOH 0.5 g
Na2WO4 x 2 H2O 4.0 mg
 
Vitamin solution (should be stored at -20°C)
Distilled water 1.0 L
p-Aminobenzonic acid 5.0 mg
Pyridoxin hydrochloride 10.0 mg
Thiamine-HCl x 2 H2O 5.0 mg
Vitamin B12 0.1 mg
Thioctic acid 5.0 mg
Riboflavin 5.0 mg
Pantothenic acid 5.0 mg
Nicotinic acid 5.0 mg
Folic acid 2.0 mg
Biotin 2.0 mg
 
Trace element solution SL-10
CoCl2·6H2O 190.0 mg
NiCl2·6H2O 24.0 mg
ZnCl2 70.0 mg
H3BO3 6.0 mg
Na2MoO4·2H2O 36.0 mg
MnCl2·4H2O 100.0 mg
HCl (25%; 7.7M) 10.0 mL
FeCl2·4H2O 1.5 g
Distilled water 990.0 mL
CuCl2·2H2O 2.0 mg
Trace element solution SL-10: First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL (This medium is modified from DSM #120: METHANOSARCINA MEDIUM)
模式菌株:
参考文献:
B2469 
Alazard, D., M. Joseph, F. Battaglia-Brunet, J. L. Cayol, and B. Ollivier. 2010. Desulfosporosinus acidiphilus sp. nov.: a moderately acidophilic sulfate-reducing bacterium isolated from acid mining drainage sediments : New taxa: Firmicutes (Class Clostridia, Order Clostridiales, Family Peptococcaceae). Extremophiles. 14: 305-312. 

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