JCM14622

价格:3200元

代购价格:询价

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JCM14622标准菌株基本信息

保藏机构: JCM
菌种名称: JCM4926, JCM 4926
英文名称: Steroidobacter denitrificans
中文名称 反硝化类固醇杆菌
存储人: Fahrbach et al.
来源历史: << DSMZ << M. Fahrbach, Univ. Aachen
其他保藏中心编号: DSM 18526; JCM 14622
分离来源: 缺氧污泥
菌种特性  
备注:  
培养条件: 28°C
生物安全等级: 1
氧气需求: 需氧
培养基: TSB培养基:815
STEROIDOBACTER MEDIUM
CaCl2·2H2O 0.15 g
Distilled water 1000 mL
KCl 0.50 g
KH2PO4 0.20 g
MgCl2·6H2O 0.40 g
Na2SO4 0.07 g
NaCl 1.00 g
NaNO3 0.42 g
NH4Cl 0.25 g
For growth with testosterone, dissolve testosterone in acetone (20 mg/mL), dispense portions of the solution, e.g. 0.1 mL portions for 10 mL medium, to anaerobic culture tubes. Let the solvent evaporate to dryness. Dispense 10 mL portions of medium and stream with N2/CO2 (80:20). Close the vessels and autoclave. Treat the vessels in an ultrasonic bath to detach and suspend the testosterone.
For growth with heptanoate, omit testosterone. Sterilize the gassed medium in anaerobic vessels by autoclaving. Add 2.5 mM heptanoate from a 20 fold filter sterilized anaerobic stock solution after cooling.
After autoclaving, add (per 10 mL) 0.3 mL NaHCO3-solution (84 g/l, autoclaved under CO2-atmosphere), 1 μl trace element solution SL 10 (see below), 1 μl selenite tungstate solution (see below), and 1 μl vitamin solution (see below). Adjust pH to 7.2. Incubate the cultures in the dark for up to 4 weeks. Briefly shake the cultures once per day.
Trace element solution SL 10
CoCl2·6H2O 190.0 mg
CuCl2·2H2O 2.0 mg
Distilled water 990.0 mL
FeCl2·4H2O 1.5 g
H3BO3 6.0 mg
MnCl2·4H2O 100.0 mg
Na2MoO4·2H2O 36.0 mg
HCl (25%; 7.7M) 10.0 mL
NiCl2·6H2O 24.0 mg
ZnCl2 70.0 mg
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 mL (This medium is modified from DSM #120: METHANOSARCINA MEDIUM)
 
Selenite tungstate solution
Distilled water 1 L
Na2SeO3·5H2O 3 mg
Na2WO4·2H2O 4 mg
NaOH 0.5 g
Solution B:
 
Vitamin solution
Vitamin B12 50 mg
Refer to BCRC Medium 801. Stir for some hours, filter sterilize the solution.
 
Pantothenic acid
Distilled water 1000 mL
α-lipoic acid 50 mg
Riboflavin 50 mg
Thiamine-HCl · 2 H2O 50 mg
Pyridoxamine-HCl 10 mg
Nicotine amide 25 mg
Nicotinic acid 25 mg
p-aminobenzoic acid 50 mg
Folic acid 20 mg
Biotin
模式菌株:  是
参考文献:
B4151 
Fahrbach, M., J. Kuever, M. Remesch, B. E. Huber, P. Kampfer, W. Dott and J. Hollender. 2008. Steroidobacter denitrificans gen. nov., sp. nov., a steroidal hormone-degrading gammaproteobacterium. Int. J. Syst. Evol. Microbiol. 58 : 2215–2223. 

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