| 产品名称： | 加富趋磁螺菌生长培养基；EMSGM培养基 |
|---|---|
| 英文名称： | Enriched Magnectic Spirillum Growth Medium；EMSGM |
| 培养基类型： | 趋磁螺菌培养基 |
| 级别： | for microbiology |
| 品牌： | ELITE-MEDIA |
| 产品目录号： | M313-01、M313-02 |
| 产品规格： | 5L、20L |
| 产品外观： | 淡黄色透明溶液 |
| 保存条件： | 密封，4°C避光保存。 |
| 注意事项： | 避免呼入和皮肤接触。 |
| 相关产品： |
Wolfe’s Trace Mineral Supplement Wolfe’s Vitamins Supplement |
产品描述：
加富趋磁螺菌生长培养基（Enriched Magnectic Spirillum Growth Medium）简称EMSGM培养基，用于培养趋磁螺菌AMB-1菌株。配方与配制方法：
| Distilled water | 1.0L |
| Wolfe’s Trace Mineral Supplement | 5.0ml |
| Wolfe’s Vitamins Supplement | 10.0ml |
| Ferric Quinate Solution | 2ml |
| 0.1% Resazum | 0.45ml |
| KH2PO4 | 0.68g |
| NaNO3 | 0.12g |
| Succinic acid | 0.74g |
| 1-cystein | 0.05g |
| Peptone | 0.2g |
| Yeast extract | 0.1g |
| pH 6.75 |
配制方法：
按照给出顺序依次加入各组分，搅拌溶解。用NaOH调节pH至6.75。121℃高温蒸汽灭菌15min。
Wolfe’s Vitamins Supplement
| 成分 | mg/L |
| Folic acid | 2.000 |
| Pyridoxine hydrochloride | 10.000 |
| Riboflavin | 5.000 |
| Biotin | 2.000 |
| Thiamine | 5.000 |
| Nicotinic acid | 5.000 |
| Pantothenic acid | 5.000 |
| Vitamin B12 | 0.100 |
| p-Aminobenzoic acid | 5.000 |
| Thioctic acid | 5.000 |
| Monopotassium phosphate | 900.000 |
Wolfe’s Trace Mineral Supplement
| 成分 | g/L |
| EDTA | 0.500 |
| MgSO4·7H2O | 3.000 |
| MnSO·H2O | 0.500 |
| NaCl | 1.000 |
| FeSO4·7H2O | 0.100 |
| Co(NO3)2·6H2O | 0.100 |
| CaCl2(anhydrous) | 0.100 |
| ZnSO4·7H2O | 0.100 |
| CuSO4·5H2O | 0.010 |
| AlK(SO4)2(anhydrous) | 0.010 |
| H3BO3 | 0.010 |
| Na2MoO4·2H2O | 0.010 |
| Na2SeO3(anhydrous) | 0.001 |
| Na2WO4·2H2O | 0.010 |
| NiCl2·6H2O | 0.020 |
| pH 6.5 |
Ferric Quinate Solution
| FeCl3 | 0.27g |
| Quinic Acid | 0.19g |
| Distilled water | 100.0ml |
121℃高温蒸汽灭菌15min。
