| 质粒名称: | pMRX-IP-GFP-LC3-RFP-LC3ΔG |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 84572 |
| 存储实验室: | Noboru Mizushima |
|
载体骨架基本信息 |
|
| 载体名称: | pMRX-IP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6100 bp |
| 完整质粒大小: | 8300 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | microtubule-associated protein 1 light chain 3 beta,Map1lc3b,LC3,LC3B |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | 2200 bp |
| 基因突变: | -- |
| GenBank编号 | NM_022867.2 |
| 启动子 | -- |
| 标签/融合蛋白: |
EGFP（插入的N端）
mRFP1（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Bgl II（销毁时未知） |
| 3' 克隆位点: | Bam HI（销毁时未知） |
| 5' 测序引物 | CGACCACTACCAGCAGAACA |
| 3' 测序引物 | AAAAGACGGCAATATGGTGG |
|
文献引用方法 |
|
| 材料和方法部分: | pMRX-IP-GFP-LC3-RFP-LC3ΔG was a gift from Noboru Mizushima (Addgene plasmid # 84572 ; http://n2t.net/addgene:84572 ; RRID:Addgene_84572) |
| 参考文献部分: | An Autophagic Flux Probe that Releases an Internal Control. Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N. Mol Cell. 2016 Nov 17;64(4):835-849. doi: 10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3. 10.1016/j.molcel.2016.09.037 PubMed 27818143 |
买家导航
pMRX-IP-GFP-LC3-RFP-LC3ΔG载体质粒基本信息
pMRX-IP-GFP-LC3-RFP-LC3ΔG质粒图谱载体图谱和pMRX-IP-GFP-LC3-RFP-LC3ΔG载体序列质粒序列多克隆位点信息
pMRX-IP-GFP-LC3-RFP-LC3ΔG质粒载体简介
pMRX-IP-GFP-LC3-RFP-LC3ΔG质粒序列载体序列
LOCUS pMRX-IP-GFP-LC3-RFP-LC3-Delta-G 8887 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses GFP-LC3-RFP-LC3-Delta-G in mammalian cells to measure
autophagic flux .
ACCESSION .
VERSION .
KEYWORDS pMRX-IP-GFP-LC3-RFP-LC3-Delta-G
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8887)
AUTHORS Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y,
Kodama A, Ishihara T, Mizushima T, Mizushima N
TITLE An Autophagic Flux Probe that Releases an Internal Control.
JOURNAL Mol Cell. 2016 Nov 17;64(4):835-849. doi:
10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
PUBMED 27818143
REFERENCE 2 (bases 1 to 8887)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8887
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..594
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 657..1014
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1079..1495
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1425..1447
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1463..1479
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind 1817..1833
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
regulatory 1915..1924
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1921..2637
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1966..1987)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2227..2246)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2574..2595
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3061..3735
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind complement(3191..3209)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3459..3478
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 3653..3676
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 4215..4767
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4359..4376)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4586..4605
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4779..5378
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4779..4798)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5275..5295
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5602..6071
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(6201..6218)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6372..6960)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6452..6471)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7124..7984)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7747..7766
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7985..8089)
/gene="bla"
/label=AmpR promoter
primer_bind 8157..8175
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
enhancer 8277..8656
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac
121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg cacccccctt
901 agaggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaata tgggcccggg
1081 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
1141 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
1201 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
1261 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
1321 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
1381 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
1441 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
1501 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
1561 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
1621 ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt
1681 ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa
1741 cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa
1801 agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg
1861 tggaccatcc tctagackgc cggrwccccc cyckagacyg ccggatctcc cgccgccacc
1921 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
1981 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
2041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
2101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
2161 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
2221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
2281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
2341 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
2401 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
2461 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
2521 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
2581 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt
2641 ggcggtggct cgggcggtgg tgggtcgggt ggcggcggat ctatcgatga attgaattcc
2701 atgccgtccg agaagacctt caaacagcgc cggagcttcg aacaaagagt ggaagatgtc
2761 cggctcatcc gggagcagca ccccaccaag atcccagtga ttatagagcg atacaagggt
2821 gagaagcagc tgcccgtcct ggacaagacc aagttccttg tacctgatca cgtgaatatg
2881 agcgaactca tcaagataat tagaaggcgc ctgcagctca atgctaacca agccttcttc
2941 ctcctggtga atgggcacag catggtgagt gtgtccacac ccatctctga agtgtacgag
3001 agcgagagag atgaagacgg cttcctgtac atggtctatg cctcccagga gacgttcggg
3061 atggcctcct ccgaggacgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
3121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
3181 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
3241 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
3301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
3361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
3421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
3481 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
3541 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
3601 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
3661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
3721 cactccaccg gcgccatcga tgaattgaat tccatgccgt ccgagaagac cttcaaacag
3781 cgccggagct tcgaacaaag agtggaagat gtccggctca tccgggagca gcaccccacc
3841 aagatcccag tgattataga gcgatacaag ggtgagaagc agctgcccgt cctggacaag
3901 accaagttcc ttgtacctga tcacgtgaat atgagcgaac tcatcaagat aattagaagg
3961 cgcctgcagc tcaatgctaa ccaagccttc ttcctcctgg tgaatgggca cagcatggtg
4021 agtgtgtcca cacccatctc tgaagtgtac gagagcgaga gagatgaaga cggcttcctg
4081 tacatggtct atgcctccca ggagacgttc taaggatccc agtgtggtgg tacgggaatt
4141 cctgcaggcc tcgagggccg gcgcgccgcg gccgctacgt aaattccgcc cccccccccc
4201 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
4261 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
4321 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
4381 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
4441 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
4501 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
4561 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
4621 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
4681 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
4741 tttcctttga aaaacacgat gataatatgg ccacaaccat gaccgagtac aagcccacgg
4801 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
4861 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
4921 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
4981 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
5041 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
5101 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
5161 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
5221 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
5281 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
5341 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
5401 gcccgaccga aaggagcgca cgaccccatg gctcgacgat aaaataaaag attttattta
5461 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
5521 taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa
5581 ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
5641 cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat
5701 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt
5761 ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga
5821 aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
5881 cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc
5941 cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg
6001 acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg
6061 ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac
6121 attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
6181 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
6241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
6301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
6361 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
6421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
6481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
6541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
6601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
6661 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
6721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
6781 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
6841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
6901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
6961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
7021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc
7081 gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7141 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
7201 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
7261 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
7321 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
7381 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
7441 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
7501 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
7561 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
7621 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
7681 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
7741 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
7801 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
7861 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
7921 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
7981 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
8041 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
8101 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
8161 ggcgtatcac gaggcccttt cgtcttcact cgaggagctt ggcccattgc atacgttgta
8221 tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca
8281 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
8341 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
8401 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
8461 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
8521 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
8581 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
8641 catcgctatt accatgctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
8701 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
8761 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
8821 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
8881 cttgtgg
//
