| 质粒名称: | pFUPI AoT5Cyp |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80280 |
| 存储实验室: | Jeremy Luban |
|
载体骨架基本信息 |
|
| 载体名称: | pFUPI |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 10306 bp |
| 完整质粒大小: | 1475 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TRIM5 CypA fusion |
| 物种来源: | Aotus monkey |
| 插入大小: | 1475 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pFUPI AoT5Cyp was a gift from Jeremy Luban (Addgene plasmid # 80280 ; http://n2t.net/addgene:80280 ; RRID:Addgene_80280) |
| 参考文献部分: | Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins engineered from human components. Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C, Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J. J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub 2009 Sep 8. 10.1172/JCI39354 PubMed 19741300 |
买家导航
pFUPI AoT5Cyp载体 pFUPIAoT5Cyp质粒基本信息
pFUPI AoT5Cyp质粒图谱 pFUPIAoT5Cyp载体图谱和pFUPI AoT5Cyp载体序列 pFUPIAoT5Cyp质粒序列多克隆位点信息
pFUPI AoT5Cyp质粒 pFUPIAoT5Cyp载体简介
pFUPI AoT5Cyp质粒序列 pFUPIAoT5Cyp载体序列
LOCUS pFUPI AoT5Cyp 11832 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses Aotus TRIM5 CypA fusion.
ACCESSION .
VERSION .
KEYWORDS pFUPI AoT5Cyp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11832)
AUTHORS Neagu MR, Ziegler P, Pertel T, Strambio-De-Castillia C, Grutter C,
Martinetti G, Mazzucchelli L, Grutter M, Manz MG, Luban J
TITLE Potent inhibition of HIV-1 by TRIM5-cyclophilin fusion proteins
engineered from human components.
JOURNAL J Clin Invest. 2009 Oct;119(10):3035-47. doi: 10.1172/JCI39354. Epub
2009 Sep 8.
PUBMED 19741300
REFERENCE 2 (bases 1 to 11832)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11832
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2611..3821
/label=UbC promoter
/note="human ubiquitin C promoter"
primer_bind 3658..3677
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
CDS 3860..4459
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3860..3879)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4356..4376
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4468..5041
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4635..4652)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4862..4881
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
misc_feature 6524..7112
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6577..6597)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6995..7006)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7115..7131)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7116..7132)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7637..7817
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7843..7860)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7849..8073
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8119..8547
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8206..8225)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8416..8437
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8556..8576)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8561..8890
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8741..8876
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8803..8822
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8938..8985
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9004..9378
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 9508..9629
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9545..9564)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9599..9618
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9678..9694)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9678..9694)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9691..9713)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9702..9718
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9726..9756)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9771..9792
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9909..9926)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10080..10668)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10160..10179)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10839..11699)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11462..11481
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11700..11804)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
3841 gactctagct agagccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
3901 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
3961 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
4021 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
4081 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
4141 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
4201 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
4261 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
4321 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
4381 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
4441 ccgcaagccc ggtgcctgag aattccgccc ctctccctcc ccccccccta acgttactgg
4501 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
4561 gccgtctttt ggcaatgtga gggcccggaa acctggtcct gtcttcttga cgagcattcc
4621 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
4681 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
4741 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
4801 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
4861 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
4921 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
4981 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
5041 accggtggtc tagaatggct tccagaatcc tggtcaatat aaaggaggag gtgacctgcc
5101 ccatctgcct ggaactcctg acagaacccc tgagcctgga ctgtggccat agcttctgcc
5161 aagcatgcat cactgcaaat cacaaaaagt ctatgccaca ccaaggagag agaagctgcc
5221 ctttgtgccg gatcagttac tcgtctgaga acctgcggcc taatcggcat ttggtcaaca
5281 tagtggagag gctcagggag gtcatgctga gcccagagga ggggcagaag gttgatcact
5341 gtgcacacca tggagagaaa cttgtactct tctgtcagca ggatggaaat gtcatttgct
5401 ggctttgtga gcggtctcaa gaacaccgtg ggcaccagac attccttgtg gaggaggttg
5461 cacagaaata ccgagaaaag ctccaggtag ctctggagat gatgaggcag aagcagaagg
5521 atgctgaaaa gttggaagct gacgtcagag aagagcaagc ttcctggaag attcaaatac
5581 aaaatgacaa aaccaacatc atggcagagt ttaaaaaacg gagagacatc ctggactgtg
5641 aggagagcaa agagttgcaa aacctggaga aggaggagaa aaacattctg aaaagacttg
5701 tacagtctga aaatgacatg gtgctgcaga cccagtccgt gagagtgctc atctcagatc
5761 tggagcatcg cctgcagggg tcagtgatgg agctgttaca gggtgtggat ggtgtcataa
5821 aaaggattga gaaagtgact ttgcagaatc caaaaacctt tcttaatgaa aaaaggagaa
5881 tatttcaaac tcctgatctg aaaggaacac tacaagtgtt taaagagccg acagaagtcc
5941 aacgctactg ggacgccgcc gcctgggacc ttgtagcatc agccatggtc aatcctactg
6001 tgttcttcga cattgccgtc gatggcgagc ccttgggccg cgtctccttc gagctgtttg
6061 cagacaaggt tccaaagaca gcagaaaact ttcgtgctct gagcactgga gagaaaggat
6121 ttggttataa gggttcctgc tttcacagaa ttattccagg gtttatgtgt cagggtggtg
6181 acttcacacg ccataatggc actggtggca agtccatcta cggggagaaa tttgatgatg
6241 agaacttcat cctaaagcat acaggtcccg gtatcttgtc catggcaaat gctggaccca
6301 acacaaacgg ttcccagttt ttcatctgca ctgccaagac tgagtggttg gatggcaagc
6361 atgtggtctt tggcaaggtg aaagaaggca tgaatgttgt ggaggccatg gagcgctttg
6421 ggtgcaggta tggcaagacc agcaaaaaga tcaccattgc tgactgtgga caactttaag
6481 gatccgttaa cggcgcgccg aattcgatat caagcttatc gataatcaac ctctggatta
6541 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
6601 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
6661 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
6721 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
6781 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
6841 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
6901 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
6961 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
7021 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
7081 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga
7141 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
7201 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
7261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
7321 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
7381 aggctacttc cctgattggc agaactacac accagggcca gggatcagat atccactgac
7441 ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga
7501 aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
7561 agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct
7621 gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
7681 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
7741 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
7801 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
7861 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
7921 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
7981 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
8041 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
8101 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
8161 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
8221 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
8281 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
8341 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
8401 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
8461 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
8521 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
8581 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
8641 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
8701 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
8761 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
8821 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
8881 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
8941 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
9001 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
9061 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
9121 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
9181 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
9241 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
9301 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
9361 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
9421 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
9481 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
9541 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
9601 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
9661 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
9721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
9781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
9841 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
9901 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
9961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
10021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
10081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
10141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
10201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
10261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
10321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
10381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
10441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
10501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
10561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
10621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
10681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
10741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
10801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
10861 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
10921 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
10981 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
11041 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
11101 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
11161 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
11221 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
11281 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
11341 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
11401 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
11461 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
11521 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
11581 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
11641 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
11701 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
11761 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
11821 gtgccacctg ac
//
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