| 质粒名称: | Sec61β-Flag-APEX2-SOPP3 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 229072 |
| 存储实验室: | Weijun Pan |
|
载体骨架基本信息 |
|
| 载体名称: | PCS2 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | Express APEX2-SOPP3 in the ER |
| 物种来源: | H. sapiens (human) |
| 启动子: | CMV |
| 克隆方法: | 其他 |
| 5’测序引物 |
ATGCCTGGACCCGCAGCTAG
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | Sec61β-Flag-APEX2-SOPP3 was a gift from Weijun Pan (Addgene plasmid # 229072 ; http://n2t.net/addgene:229072 ; RRID:Addgene_229072) |
| 参考文献部分: | Photoactivated SOPP3 enables APEX2-mediated proximity labeling with high spatio-temporal resolution in live cells. Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang W, Pan W. Cell Res. 2025 Feb;35(2):149-152. doi: 10.1038/s41422-024-01061-9. Epub 2024 Dec 10. 10.1038/s41422-024-01061-9 PubMed 39653757 |
买家导航
Sec61β-Flag-APEX2-SOPP3载体质粒基本信息
Sec61β-Flag-APEX2-SOPP3质粒图谱载体图谱和Sec61β-Flag-APEX2-SOPP3载体序列质粒序列多克隆位点信息
Sec61β-Flag-APEX2-SOPP3质粒载体简介
Sec61β-Flag-APEX2-SOPP3质粒序列载体序列
LOCUS Sec61β-Flag-APEX2-SOPP3 5492 bp DNA circular SYN 14-APR-2025
DEFINITION Expresse APEX2-SOPP3 in ER in mammalian cell.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5492)
AUTHORS Qu D, Li Y, Liu Q, Cao B, Cao M, Lin X, Shen C, Zou P, Zhou H, Zhang
W, Pan W
TITLE Photoactivated SOPP3 enables APEX2-mediated proximity labeling with
high spatio-temporal resolution in live cells.
JOURNAL Cell Res. 2025 Feb;35(2):149-152. doi: 10.1038/s41422-024-01061-9.
Epub 2024 Dec 10.
PUBMED 39653757
REFERENCE 2 (bases 1 to 5492)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41422-024-01061-9"; journalName: "Cell Res"; date:
"2025-02"; volume: "35"; issue: "2"; pages: "149-152"
COMMENT Sequence Label: Sec61-beta-Flag-APEX2-SOPP3
FEATURES Location/Qualifiers
source 1..5492
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 891..909
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 891..908
/label=SP6
/note="SP6 promoter, forward primer"
CDS 1238..1261
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
polyA_signal 2400..2534
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2424..2443)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2478..2497
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2647..2667)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(2647..2665)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2686..2702)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2686..2702)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2699..2721)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2710..2726
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(2734..2740,2741..2758,2759..2764))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2779..2800
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2917..2934)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3088..3676)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3168..3187)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3847..4638,4639..4707))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4470..4489
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4708..4812)
/gene="bla"
/label=AmpR promoter
rep_origin complement(4838..5293)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4975..4996)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5187..5206
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter join(5364..5492,1..856)
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
61 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
121 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
181 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
241 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
301 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
361 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
421 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
481 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
541 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
601 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
661 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
721 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
781 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
841 tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg atttaggtga
901 cactatagaa tacaagctac ttgttctttt tgcaggatcc atgcctggac ccgcagctag
961 tgcaacaaat gttggtgcct ccagccgttc ccccagtaag acggtggctc cccgcactgc
1021 tggtacctca gccagacaaa ggaaagccac aagcagcagt gcacgcagcg gaggcagatc
1081 cacagcttct gcaggcacag gaggaatgtg gcgcttttac actgaagatt caccagggct
1141 taaagttggc ccagttccag ttttggtgat gagtctgctg tttatcgcat ctgtcttcat
1201 gctgcacatc tggggaaagt acacccgctc cgctagcgac tacaaggatg acgatgacaa
1261 agaattcggg aaatcatacc caacagtgtc cgcagactac caggatgccg tggagaaagc
1321 caagaagaag ctgagagggt ttatcgcaga aaagaggtgc gcacctctga tgctgagact
1381 ggctttccac agcgcaggca cctttgacaa gggaaccaaa acaggcggac cctttggaac
1441 aatcaagcac cctgctgaac tggcacatag tgccaacaat gggctggaca tcgccgtgcg
1501 gctgctggaa cctctgaaag cagagttccc aattctgtcc tacgccgatt tttatcagct
1561 ggcaggagtg gtcgctgtgg aggtcactgg gggccccaag gtgcctttcc acccaggacg
1621 ggaggacaaa ccagaaccac ctccagaggg gcgcctgcca gatccgacaa agggctccga
1681 ccatctgcga gatgtgtttg ggaaagctat gggcctgact gaccaggata tcgtcgcact
1741 gtctggaggg cacaccattg gcgccgctca taaggaaagg tcaggcttcg agggaccctg
1801 gacaagcaac cccctgattt tcgacaattc ttactttact gaactgctga gtggagagaa
1861 ggaagggctg ctgcagctgc ccagcgacaa agccctgctg tccgatcccg tgttcagacc
1921 tctggtcgat aagtatgcag ccgacgagga tgcttttttc gcagattacg cagaagcaca
1981 tcagaagctg tcagaactgg gatttgccga cgccaccggt ggcggtggcg gatcgactag
2041 tatggagaaa agtttcgtga taactgatcc acggctgcca gacaatccca tcatcttcgc
2101 atccgatggc ttcctggagc tgaccgagta ttccagagag gagatcctgg gccgcaatgg
2161 ccgctttctg cagggaccag agacagacca ggccacagtg cagaagattc gcgatgccat
2221 tagagatcag cgcgagatta ccgtgcagct gataaactac acaaaaagcg ggaagaaatt
2281 cttgaacctc ctgaacctcc agcccattag ggaccagaag ggtgagctcc aggctttcat
2341 cggagtggtg ctggatggat agctcgagcc tctagaacta tagtgagtcg tattacgtag
2401 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
2461 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
2521 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
2581 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt
2641 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
2701 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
2761 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
2821 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
2881 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
2941 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
3001 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
3061 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
3121 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
3181 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
3241 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
3301 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
3361 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
3421 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
3481 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
3541 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
3601 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
3661 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
3721 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
3781 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
3841 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
3901 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
3961 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
4021 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
4081 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
4141 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
4201 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
4261 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
4321 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
4381 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
4441 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
4501 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
4561 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
4621 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
4681 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
4741 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
4801 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
4861 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
4921 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
4981 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
5041 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
5101 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
5161 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
5221 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
5281 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
5341 cgggcctctt cgctattacg ccagtcgacc gccaattcaa tatggcgtat atggactcat
5401 gccaattcaa tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt
5461 gccaattcaa tatggtggat ctggacccca gc
//
