pNL1.2[NlucP]

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pNL1.2[NlucP]
质粒类型: 荧光素酶载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 3233 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pNL1.2[NlucP]载体图谱

载体简介

载体序列

LOCUS       pNL1.2[NlucP]              3233 bp ds-DNA     circular SYN 26-JUL-2013
DEFINITION  Promoterless vector encoding destabilized NanoLuc(R) luciferase for 
            measuring the activity of promoter and enhancer sequences.
ACCESSION   JQ437371
VERSION     .
KEYWORDS    pNL1.2[NlucP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, February 26, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..70
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..612
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /note="Nluc"
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             616..735
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /note="hPEST"
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    787..908
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1327..1915)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2115..2975)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    3080..3128
                     /note="synthetic polyadenylation signal"
     misc_feature    3142..3233
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
      121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
      181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
      241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
      301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
      361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
      421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
      481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
      541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
      601 cgcattctgg cgaattctca cggctttccg cctgaggttg aagagcaagc cgccggtaca
      661 ttgcctatgt cctgcgcaca agaaagcggt atggaccggc acccagccgc ttgtgcttca
      721 gctcgcatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
      781 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      841 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      901 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      961 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     1021 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     1081 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     1141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1741 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     2101 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     2161 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     2221 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     2281 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     2341 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     2401 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     2461 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     2521 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     2581 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     2641 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     2701 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     2761 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     2821 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
     2881 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
     2941 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
     3001 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
     3061 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     3121 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     3181 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//

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