pGL4.14[luc2/Hygro]

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pGL4.14[luc2/Hygro]
质粒类型: 荧光素酶载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 5841 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pGL4.14[luc2Hygro]载体图谱

载体简介

载体序列

LOCUS       pGL4.14[luc2 Hygro]                5841 bp ds-DNA     circular SYN 29-JUN-2013
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a luciferase assay.
ACCESSION   AY864928
VERSION     .
KEYWORDS    pGL4.14[luc2 Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..70
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1752
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1796..1917
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2111..2468
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2499..3536
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3560..3608
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3935..4523)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4723..5583)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5688..5736
                     /note="synthetic polyadenylation signal"
     misc_feature    5750..5841
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1801 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1861 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1921 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     1981 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg
     2041 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     2101 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2461 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gaagaagccc gaactcaccg
     2521 ctaccagcgt tgaaaaattt ctcatcgaga agttcgacag tgtgagcgac ctgatgcagt
     2581 tgtcggaggg cgaagagagc cgagccttca gcttcgatgt cggcggacgc ggctatgtac
     2641 tgcgggtgaa tagctgcgct gatggcttct acaaagaccg ctacgtgtac cgccacttcg
     2701 ccagcgctgc actacccatc cccgaagtgt tggacatcgg cgagttcagc gagagcctga
     2761 catactgcat cagtagacgc gcccaaggcg ttactctcca agacctcccc gaaacagagc
     2821 tgcctgctgt gttacagcct gtcgccgaag ctatggatgc tattgccgcc gccgacctca
     2881 gtcaaaccag cggcttcggc ccattcgggc cccaaggcat cggccagtac acaacctggc
     2941 gggatttcat ttgcgccatt gctgatcccc atgtctacca ctggcagacc gtgatggacg
     3001 acaccgtgtc cgccagcgta gctcaagccc tggacgaact gatgctgtgg gccgaagact
     3061 gtcccgaggt gcgccacctc gtccatgccg acttcggcag caacaacgtc ctgaccgaca
     3121 acggccgcat caccgccgta atcgactggt ccgaagctat gttcggggac agtcagtacg
     3181 aggtggccaa catcttcttc tggcggccct ggctggcttg catggagcag cagactcgct
     3241 acttcgagcg ccggcatccc gagctggccg gcagccctcg tctgcgagcc tacatgctgc
     3301 gcatcggcct ggatcagctc taccagagcc tcgtggacgg caacttcgac gatgctgcct
     3361 gggctcaagg ccgctgcgat gccatcgtcc gcagcggggc cggcaccgtc ggtcgcacac
     3421 aaatcgctcg ccggagcgca gccgtatgga ccgacggctg cgtcgaggtg ctggccgaca
     3481 gcggcaaccg ccggcccagt acacgaccgc gcgctaagga ggtaggtcga gtttaaactc
     3541 tagaaccggt catggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     3601 tttgtgtgtt cgaactagat gctgtcgacc gatgcccttg agagccttca acccagtcag
     3661 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     3721 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
     3781 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3841 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3901 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4141 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4261 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4321 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     4381 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4441 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4501 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4561 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4621 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4681 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
     4741 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
     4801 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
     4861 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
     4921 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
     4981 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
     5041 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
     5101 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
     5161 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
     5221 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
     5281 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
     5341 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
     5401 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
     5461 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
     5521 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
     5581 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
     5641 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
     5701 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     5761 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     5821 caggtgccag aacatttctc t
//

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