pGL4.12[luc2CP]

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pGL4.12[luc2CP]
质粒类型: 荧光素酶载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 4421 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: 编码高度不稳定荧光素酶的无启动子载体,用于测定启动子和增强子序列的活性。
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pGL4.12[luc2CP]载体图谱

载体简介

载体序列

LOCUS       pGL4.12[luc2CP]              4421 bp ds-DNA     circular SYN 29-JUN-2013
DEFINITION  Promoterless vector encoding highly destabilized luciferase for 
            measuring the activity of promoter and enhancer sequences.
ACCESSION   AY738224
VERSION     .
KEYWORDS    pGL4.12[luc2CP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, June 13, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..70
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1756..1803
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /note="hCL1"
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             1807..1926
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /note="hPEST"
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1975..2096
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2515..3103)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3303..4163)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4268..4316
                     /note="synthetic polyadenylation signal"
     misc_feature    4330..4421
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctgcttg caagaactgg ttcagtagct taagccactt tgtgatccac
     1801 cttaacagcc acggcttccc tcccgaggtg gaggagcagg ccgccggcac cctgcccatg
     1861 agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag cgccaggatc
     1921 aacgtctaac tgcagtctag agtcggggcg gccggccgct tcgagcagac atgataagat
     1981 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     2041 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     2101 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     2161 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     2221 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     2281 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     2341 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2401 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2461 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2521 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2581 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2641 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2701 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2761 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2821 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2881 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2941 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3001 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3061 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3121 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3181 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3241 ctaaagtata tatgagtaaa cttggtctga cagcggccgc aaatgctaaa ccactgcagt
     3301 ggttaccagt gcttgatcag tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc
     3361 atagtggcct gactccccgt cgtgtagatc actacgattc gtgagggctt accatcaggc
     3421 cccagcgcag caatgatgcc gcgagagccg cgttcaccgg cccccgattt gtcagcaatg
     3481 aaccagccag cagggagggc cgagcgaaga agtggtcctg ctactttgtc cgcctccatc
     3541 cagtctatga gctgctgtcg tgatgctaga gtaagaagtt cgccagtgag tagtttccga
     3601 agagttgtgg ccattgctac tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg
     3661 ttcaactctg gttcccagcg gtcaagccgg gtcacatgat cacccatatt atgaagaaat
     3721 gcagtcagct ccttagggcc tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg
     3781 ctcatggtaa tggcagcact acacaattct cttaccgtca tgccatccgt aagatgcttt
     3841 tccgtgaccg gcgagtactc aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc
     3901 tgctcttgcc cggcgtctat acgggacaac accgcgccac atagcagtac tttgaaagtg
     3961 ctcatcatcg ggaatcgttc ttcggggcgg aaagactcaa ggatcttgcc gctattgaga
     4021 tccagttcga tatagcccac tcttgcaccc agttgatctt cagcatcttt tactttcacc
     4081 agcgtttcgg ggtgtgcaaa aacaggcaag caaaatgccg caaagaaggg aatgagtgcg
     4141 acacgaaaat gttggatgct catactcgtc ctttttcaat attattgaag catttatcag
     4201 ggttactagt acgtctctca aggataagta agtaatatta aggtacggga ggtattggac
     4261 aggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     4321 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     4381 ggctgtcccc agtgcaagtg caggtgccag aacatttctc t
//

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