pLenti X2 Neo DEST (w18-1)

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

质粒名称: pLenti X2 Neo DEST (w18-1) 
出品公司: Addgene
目录编号: 17390
存储实验室: Eric Campeau

载体骨架基本信息
载体名称: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
载体抗性: 氨苄霉素、氯霉素
空载体大小: 9249 bp
载体类型: 慢病毒表达
筛选标记: 新霉素(G418)
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 克隆位点:  
3' 克隆位点:  
5' 测序引物 n/a
3' 测序引物  

文献引用方法
材料和方法部分: pLenti X2 Neo DEST (w18-1) was a gift from Eric Campeau (Addgene plasmid # 17390)
参考文献部分: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

载体质粒图谱和多克隆位点信息

pLenti X2 Neo DEST (w18-1)载体图谱

载体简介

载体序列

LOCUS       pLenti X2 Neo DEST (w18-1)	9249 bp 	DNA	SYN
DEFINITION  pLenti X2 Neo DEST (w18-1)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="pLenti X2 Neo DEST (w18-1)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..3668
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     CDS             3629..4219
                     /label="ORF frame 2"
     misc_feature    4016..4031
                     /label="cPPT"
     misc_feature    4185..4760
                     /label="WPRE"
     CDS             4273..4806
                     /label="ORF frame 1"
     misc_feature    complement(4849..4872)
                     /label="MSCV_rev_primer"
     misc_feature    5246..5265
                     /label="mPGK_F_primer"
     gene            5327..6127
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             5327..6130
                     /label="ORF frame 2"
     misc_feature    6204..6219
                     /label="cPPT"
     misc_feature    6204..6225
                     /label="U3PPT"
     misc_feature    complement(6288..6388)
                     /label="attR2"
     misc_feature    6288..6388
                     /label="attR1"
     misc_feature    complement(6429..6734)
                     /label="ccdB"
     gene            complement(7076..7735)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             complement(7076..7735)
                     /label="ORF frame 3"
     misc_feature    7844..7944
                     /label="attR2"
     misc_feature    complement(7844..7968)
                     /label="attR1"
     misc_feature    8043..8223
                     /label="HIV-1_5_LTR"
     misc_feature    8043..8223
                     /label="truncHIV-1_3_LTR"
     terminator      8298..8417
                     /label="SV40_PA_terminator"
     misc_feature    8386..8405
                     /label="EBV_rev_primer"
     rep_origin      8435..8512
                     /label="SV40_origin"
     misc_feature    8497..8516
                     /label="SV40pro_F_primer"
     promoter        complement(8603..8621)
                     /label="T7_promoter"
     promoter        complement(8631..8647)
                     /label="M13_forward20_primer"
     misc_feature    complement(8640..8662)
                     /label="M13_pUC_fwd_primer"
     misc_feature    8640..8783
                     /label="lacZ_a"
     rep_origin      8809..9115
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
 3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
 3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
 3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
 3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
 3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
 3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggttaa cttttaaaag
 4021 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
 4081 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta tTCCAGTGTG
 4141 GTGGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG Acaatcaacc tctggattac
 4201 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
 4261 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
 4321 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
 4381 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
 4441 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
 4501 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
 4561 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
 4621 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
 4681 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
 4741 agtcggatct ccctttgggc cgcctccccg cctggAATTC TGCAGATATC CGGACTAGTG
 4801 ATCTAATTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC AGTCTGGAGC ATGCGCTTTA
 4861 GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT GGCCTCGCAC ACATTCCACA
 4921 TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC CCTTCGCGCC ACCTTCTACT
 4981 CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG CGTCGTGCAG GACGTGACAA
 5041 ATGGAAGTAG CACGTCTCAC TAGTCTCGTG CAGATGGACA GCACCGCTGA GCAATGGAAG
 5101 CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT CCTTCGCTTT CTGGGCTCAG
 5161 AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG GGCTCAGGGG CGGGGCGGGC
 5221 GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC AAAAGCGCAC GTCTGCCGCG
 5281 CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTGCA GCCAATATGG GATCGGCCAT
 5341 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
 5401 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
 5461 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA
 5521 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
 5581 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
 5641 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
 5701 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
 5761 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
 5821 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA
 5881 GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG
 5941 CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC
 6001 GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT
 6061 GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA
 6121 GTTCTTCTGA GGGGATCCGT CGAGGGCCCG GTACctttaa gaccaatgac ttacaaggca
 6181 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggCT AGACTCGAGC
 6241 GGCCGCCACT GTGCTGGATA TCAACCACTT TGTACAAGAA AGCTGAACGA GAAACGTAAA
 6301 ATGATATAAA TATCAATATA TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT
 6361 GTAAAACACA ACATATCCAG TCACTATGGT CGACCTGCAG ACTGGCTGTG TATAACGGAG
 6421 CCTGACATTT ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG
 6481 TGGCTGAGAT CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG
 6541 GTCATCATGC GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT
 6601 TTATCTGACA GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA
 6661 ATAATATCAC TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA
 6721 ACCTTAAACT GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT
 6781 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG
 6841 ACGACGGGCT TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT
 6901 GCCTTGAGCA ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA
 6961 CACCTCTTTT TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT
 7021 CAGCGCGCAA ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA GATCTTTACG
 7081 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA
 7141 AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT
 7201 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT
 7261 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA
 7321 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA
 7381 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG
 7441 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT
 7501 CTTTCATTGC CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA
 7561 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA
 7621 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT
 7681 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG
 7741 CTTCCTTAGC TCCTGAAAAT CTCGCCGGAT CCTAACTCAA AATCCACACA TTATACGAGC
 7801 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGCGGC CGCCATAGTG ACTGGATATG
 7861 TTGTGTTTTA CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT
 7921 ATTTATATCA TTTTACGTTT CTCGTTCAGC TTTTTTGTAC AAACTTGTTG ATATCTGCAG
 7981 AATTCCACCA CACTGGACTA Gctcactccc aacgaagaca agatctgctt tttgcttgta
 8041 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctgc ctagggaacc
 8101 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
 8161 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
 8221 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
 8281 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
 8341 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
 8401 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc
 8461 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
 8521 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
 8581 cgtcgagacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
 8641 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
 8701 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
 8761 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg
 8821 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
 8881 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
 8941 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
 9001 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
 9061 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
 9121 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
 9181 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
 9241 acaatttcc
//

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