pLenti PGK Puro DEST (w529-2)

价格:1200元

联系方式:I47-825O-882O

买家导航

载体基本信息

质粒名称: pLenti PGK Puro DEST (w529-2) 
出品公司: Addgene
目录编号: 19068
存储实验室: Eric Campeau

载体骨架基本信息
载体名称: p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
载体抗性: 氨苄霉素、氯霉素
空载体大小: 9599 bp
载体类型: 哺乳细胞表达
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
克隆方法: 限制性内切酶
5' 测序引物 CMV-F
3' 测序引物  

文献引用方法
材料和方法部分: pLenti PGK Puro DEST (w529-2) was a gift from Eric Campeau (Addgene plasmid # 19068)
参考文献部分: A versatile viral system for expression and depletion of proteins in mammalian cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394

载体质粒图谱和多克隆位点信息

pLenti PGK Puro DEST (w529-2)载体图谱

载体简介

载体序列

LOCUS       pLenti PGK Puro DEST (w529-2)	9599 bp 	DNA	SYN
DEFINITION  pLenti PGK Puro DEST (w529-2)
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="pLenti PGK Puro DEST (w529-2)"
                     /mol_type="other DNA"
     promoter        62..90
                     /label="AmpR_promoter"
     gene            132..992
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             132..992
                     /label="ORF frame 3"
     rep_origin      1147..1766
                     /label="pBR322_origin"
     promoter        2075..2104
                     /label="lac_promoter"
     misc_feature    2118..2140
                     /label="M13_pUC_rev_primer"
     promoter        2139..2157
                     /label="M13_reverse_primer"
     promoter        2174..2193
                     /label="T3_promoter"
     misc_feature    2448..2628
                     /label="truncHIV-1_3_LTR"
     misc_feature    2448..2628
                     /label="HIV-1_5_LTR"
     misc_feature    2736..2780
                     /label="HIV-1_psi_pack"
     CDS             3168..4376
                     /label="ORF frame 3"
     misc_feature    3290..3523
                     /label="RRE"
     misc_feature    4023..4038
                     /label="cPPT"
     misc_feature    4746..4870
                     /label="attR1"
     misc_feature    complement(4770..4870)
                     /label="attR2"
     promoter        4895..4918
                     /label="lac_promoter"
     gene            4979..5638
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             4979..5638
                     /label="ORF frame 2"
     misc_feature    5980..6285
                     /label="ccdB"
     misc_feature    6326..6426
                     /label="attR2"
     misc_feature    complement(6326..6433)
                     /label="attR1"
     misc_feature    6483..7058
                     /label="WPRE"
     CDS             6571..7200
                     /label="ORF frame 1"
     misc_feature    complement(7118..7141)
                     /label="MSCV_rev_primer"
     CDS             7363..8202
                     /label="ORF frame 1"
     misc_feature    7515..7534
                     /label="mPGK_F_primer"
     CDS             complement(7528..8253)
                     /label="ORF frame 3"
     gene            7603..8202
                     /label="puro (variant)"
                     /gene="puro (variant)"
     misc_feature    8323..8344
                     /label="U3PPT"
     misc_feature    8323..8338
                     /label="cPPT"
     misc_feature    8393..8573
                     /label="truncHIV-1_3_LTR"
     misc_feature    8393..8573
                     /label="HIV-1_5_LTR"
     terminator      8648..8767
                     /label="SV40_PA_terminator"
     misc_feature    8736..8755
                     /label="EBV_rev_primer"
     rep_origin      8785..8862
                     /label="SV40_origin"
     misc_feature    8847..8866
                     /label="SV40pro_F_primer"
     promoter        complement(8953..8971)
                     /label="T7_promoter"
     promoter        complement(8981..8997)
                     /label="M13_forward20_primer"
     misc_feature    complement(8990..9012)
                     /label="M13_pUC_fwd_primer"
     misc_feature    8990..9133
                     /label="lacZ_a"
     rep_origin      9159..9465
                     /label="f1_origin"
ORIGIN
    1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
   61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
  121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
  181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
  241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
  301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
  361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
  421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
  481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
  541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
  601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
  661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
  721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
  781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
  841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
  901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
  961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
 2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
 2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
 2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
 2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
 2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
 2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
 2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
 2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
 2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
 2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
 2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
 2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
 3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
 3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
 3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
 3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
 3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
 3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
 3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
 3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
 3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
 3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
 3601 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
 3661 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
 3721 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
 3781 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
 3841 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
 3901 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
 3961 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
 4021 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
 4081 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
 4141 atcacgagac tagcctcgag aagcttgata tcgaattccc acggggttgg ggttgcgcct
 4201 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
 4261 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
 4321 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
 4381 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
 4441 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
 4501 tgtggccaat agcggctgct cagcggggcg cgccgagagc agcggccggg aaggggcggt
 4561 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
 4621 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
 4681 cctctctccc cagggggatc accggTCGTC GACTAGTCCA GTGTGGTGGA ATTCTGCAGA
 4741 TATCAACAAG TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA
 4801 TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC
 4861 AGTCACTATG GCGGCCGCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA
 4921 TAATGTGTGG ATTTTGAGTT AGGATCCGGC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT
 4981 GGAGAAAAAA ATCACTGGAT ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA
 5041 TTTTGAGGCA TTTCAGTCAG TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT
 5101 TACGGCCTTT TTAAAGACCG TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA
 5161 CATTCTTGCC CGCCTGATGA ATGCTCATCC GGAATTCCGT ATGGCAATGA AAGACGGTGA
 5221 GCTGGTGATA TGGGATAGTG TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC
 5281 GTTTTCATCG CTCTGGAGTG AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC
 5341 GCAAGATGTG GCGTGTTACG GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA
 5401 TATGTTTTTC GTCTCAGCCA ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC
 5461 CAATATGGAC AACTTCTTCG CCCCCGTTTT CACCATGGGC AAATATTATA CGCAAGGCGA
 5521 CAAGGTGCTG ATGCCGCTGG CGATTCAGGT TCATCATGCC GTCTGTGATG GCTTCCATGT
 5581 CGGCAGAATG CTTAATGAAT TACAACAGTA CTGCGATGAG TGGCAGGGCG GGGCGTAAAG
 5641 ATCTGGATCC GGCTTACTAA AAGCCAGATA ACAGTATGCG TATTTGCGCG CTGATTTTTG
 5701 CGGTATAAGA ATATATACTG ATATGTATAC CCGAAGTATG TCAAAAAGAG GTGTGCTATG
 5761 AAGCAGCGTA TTACAGTGAC AGTTGACAGC GACAGCTATC AGTTGCTCAA GGCATATATG
 5821 ATGTCAATAT CTCCGGTCTG GTAAGCACAA CCATGCAGAA TGAAGCCCGT CGTCTGCGTG
 5881 CCGAACGCTG GAAAGCGGAA AATCAGGAAG GGATGGCTGA GGTCGCCCGG TTTATTGAAA
 5941 TGAACGGCTC TTTTGCTGAC GAGAACAGGG ACTGGTGAAA TGCAGTTTAA GGTTTACACC
 6001 TATAAAAGAG AGAGCCGTTA TCGTCTGTTT GTGGATGTAC AGAGTGATAT TATTGACACG
 6061 CCCGGGCGAC GGATGGTGAT CCCCCTGGCC AGTGCACGTC TGCTGTCAGA TAAAGTCTCC
 6121 CGTGAACTTT ACCCGGTGGT GCATATCGGG GATGAAAGCT GGCGCATGAT GACCACCGAT
 6181 ATGGCCAGTG TGCCGGTCTC CGTTATCGGG GAAGAAGTGG CTGATCTCAG CCACCGCGAA
 6241 AATGACATCA AAAACGCCAT TAACCTGATG TTCTGGGGAA TATAAATGTC AGGCTCCGTT
 6301 ATACACAGCC AGTCTGCAGG TCGACCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA
 6361 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT
 6421 TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT TGATATCCAG CACAGTGGCG GCCGCTCGAc
 6481 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
 6541 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
 6601 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
 6661 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
 6721 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
 6781 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
 6841 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
 6901 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
 6961 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
 7021 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgGAATTCTA
 7081 CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT
 7141 GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG
 7201 GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT
 7261 CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC
 7321 ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT
 7381 TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG
 7441 GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG CCCGAAGGTC
 7501 CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG TCTGCCGCGC TGTTCTCCTC
 7561 TTCCTCATCT CCGGGCCTTT CGACCTGCAG CCCAAGCTTA CCATGACCGA GTACAAGCCC
 7621 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG
 7681 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC
 7741 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
 7801 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
 7861 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
 7921 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
 7981 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
 8041 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC
 8101 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
 8161 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC CCACGACCCG
 8221 CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCTC GAGGGCCCGG TACctttaag
 8281 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
 8341 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
 8401 ctggttagac cagatctgag cctgggagct ctctggctgc ctagggaacc cactgcttaa
 8461 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
 8521 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
 8581 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
 8641 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
 8701 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
 8761 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
 8821 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
 8881 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg cgtcgagacg
 8941 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
 9001 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
 9061 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
 9121 gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
 9181 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
 9241 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
 9301 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
 9361 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
 9421 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
 9481 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
 9541 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcc

//

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