pLOX-CWBmi1

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pLOX-CWBmi1
质粒类型: 慢病毒系统载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9967bp
5' 测序引物及序列: CMV fwd: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: SP6: ATTTAGGTGACACTATAG
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: 克隆菌株为Stbl3 或 HB101。
产品目录号: 12240
稳定性: --
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLOX-CWBmi1载体图谱



载体简介

载体序列

LOCUS       pLOX-CWBmi1	9967 bp 	DNA	SYN
DEFINITION  pLOX-CWBmi1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="pLOX-CWBmi1"
                     /mol_type="other DNA"
     misc_feature    457..637
                     /label="HIV-1_5_LTR"
     misc_feature    457..637
                     /label="truncHIV-1_3_LTR"
     misc_feature    748..792
                     /label="HIV-1_psi_pack"
     CDS             1180..2028
                     /label="ORF frame 1"
                    
     misc_feature    1302..1535
                     /label="RRE"
                     
     promoter        2040..2544
                     /label="CMV_immearly_promoter"
                     
     misc_feature    2047..2334
                     /label="CAG_enhancer"
                   
     misc_feature    2501..2521
                     /label="CMV_fwd_primer"
                    
     promoter        2502..2571
                     /label="CMV_promoter"
                     
     misc_feature    2545..2564
                     /label="pCEP_fwd_primer"
                    
     misc_feature    2547..2571
                     /label="LNCX_primer"
                    
     CDS             2681..3655
                     /label="ORF frame 2"
                     
     misc_feature    3801..4393
                     /label="WPRE"
                     
     CDS             complement(3886..4389)
                     /label="ORF frame 2"
                    
     CDS             3889..4449
                     /label="ORF frame 1"
                     
     misc_feature    4459..4474
                     /label="cPPT"
                     
     misc_feature    4459..4480
                     /label="U3PPT"
                    
     misc_feature    4531..4564
                     /label="loxP"
                     
     misc_feature    4584..4764
                     /label="HIV-1_5_LTR"
                   
     misc_feature    4584..4764
                     /label="truncHIV-1_3_LTR"
                     
     misc_feature    complement(4790..4807)
                     /label="Sp6_primer"
                   
     gene            5045..5308
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     
     misc_feature    complement(5097..5116)
                     /label="pBRrevBam_primer"
                     
     misc_feature    5449..5471
                     /label="pGEX_3_primer"
                    
     promoter        5630..5658
                     /label="AmpR_promoter"
                     
     gene            5700..6560
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     
     CDS             5700..6560
                     /label="ORF frame 3"
                    
     rep_origin      6715..7334
                     /label="pBR322_origin"
                    
     misc_feature    complement(7560..7580)
                     /label="pBABE_3_primer"
                     
     misc_feature    complement(7566..8019)
                     /label="SV40_enhancer"
                   
     promoter        7578..7846
                     /label="SV40_promoter"
                    
     rep_origin      7745..7822
                     /label="SV40_origin"
                    
     misc_feature    7807..7826
                     /label="SV40pro_F_primer"
                    
     misc_feature    9027..9042
                     /label="SV40_int"
                    
     misc_feature    9048..9095
                     /label="SV40_3_splice"
                    
     CDS             9172..9645
                     /label="ORF frame 1"
                    
     misc_feature    9223..9243
                     /label="SV40 NLS"
                     
     misc_feature    9229..9243
                     /label="NLS"
                     
     terminator      9671..9802
                     /label="SV40_PA_terminator"
                   
     misc_feature    9759..9778
                     /label="EBV_rev_primer"
                     
ORIGIN
    1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
   61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
  121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
  181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
  241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
  301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
  361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
  421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
  481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
  541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
  601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
  661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
  721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
  781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
  841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
  901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
  961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc
 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctgata
 2641 tcatcgatga attctttccg ggatctttta tcaagcagaa atgcatcgaa caaccagaat
 2701 caagatcact gagctaaatc cccacttaat gtgtgtcctg tgtggagggt acttcattga
 2761 tgccactacc ataatagaat gtctacattc cttctgtaaa acatgtattg tacgttactt
 2821 ggagaccagc aagtattgtc ctatttgtga tgtccaggtt cacaaaacca gaccactcct
 2881 gaacataagg tcagataaaa ctcttcaaga tattgtatac aaattagtcc cagggctttt
 2941 caaaaatgag atgaagagaa gaagagattt ttatgcagct cacccgtcag ctgatgctgc
 3001 caatggctcc aatgaagacc gaggagaagt tgcagatgag gagaagagga ttataactga
 3061 tgatgagata ataagcttgt ctattgagtt ctttgatcag agcagattgg atcggaaagt
 3121 aaataaagag aagcctaagg aagaggtgaa tgataaaagg tacttacgat gcccagcagc
 3181 aatgactgtg atgcacttga gaaagtttct caggagtaaa atggacatac ccaatacttt
 3241 ccagattgac gtcatgtatg aagaggaacc tttaaaggat tactacacgc taatggacat
 3301 tgcctacatt tatacctgga gaagaaatgg cccactacct ttgaaataca gagttcggcc
 3361 aacttgcaaa agaatgaaga tgagtcacca gagggatgga ctgacgaatg ctggagagct
 3421 ggaaagtgac tctgggagtg acaaggccaa cagcccagcc ggaggtgttc cctccacctc
 3481 ttcctgtttg cccagtccca gcactccagt gcagtctcct cacccacagt tccctcacat
 3541 ttccagtacc atgaatggaa ccagcaacag ccccagtgct aaccaccaat cttcctttgc
 3601 cagtagacct cgaaaatcat cactaaatgg gtcatcagca acttcatctg gttaggactg
 3661 ttaaggaaaa gatttttcaa ccccctgatt tagttacctt cattcattac agctttatag
 3721 atgcttaata catgtgactg tcgtccagtt tgcttccttt tgtagtgact ttaaatttct
 3781 gctcgaggga attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
 3841 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
 3901 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
 3961 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
 4021 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
 4081 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
 4141 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
 4201 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
 4261 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
 4321 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
 4381 tccccgcatc gggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
 4441 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
 4501 aagacaagat gggatcaatt caccatggga ataacttcgt atagcataca ttatacgaag
 4561 ttatgctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
 4621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
 4681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
 4741 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
 4801 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
 4861 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
 4921 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
 4981 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
 5041 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
 5101 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
 5161 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
 5221 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
 5281 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
 5341 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
 5401 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
 5461 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
 5521 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
 5581 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
 5641 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
 5701 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
 5761 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
 5821 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
 5881 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
 5941 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
 6001 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
 6061 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
 6121 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
 6181 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
 6241 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
 6301 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
 6361 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
 6421 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
 6481 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
 6541 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
 6601 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
 6661 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
 6721 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
 6781 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
 6841 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
 6901 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
 6961 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
 7021 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
 7081 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
 7141 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
 7201 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
 7261 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
 7321 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
 7381 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
 7441 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
 7501 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
 7561 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
 7621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
 7681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
 7741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
 7801 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
 7861 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
 7921 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
 7981 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
 8041 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
 8101 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
 8161 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
 8221 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
 8281 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
 8341 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
 8401 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
 8461 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
 8521 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
 8581 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
 8641 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
 8701 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
 8761 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
 8821 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
 8881 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
 8941 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
 9001 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
 9061 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
 9121 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
 9181 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
 9241 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
 9301 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
 9361 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
 9421 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
 9481 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
 9541 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
 9601 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
 9661 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
 9721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
 9781 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
 9841 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
 9901 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
 9961 acttagt
//

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