pEF1α-Tet3G

LOCUS       pEF1α-Tet3G                7859 bp DNA     circular SYN 11-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2025 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        206..1387
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          436..1378
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     CDS             1495..2241
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     polyA_signal    2251..2385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      complement(3105..3693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(3864..4724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 3864 .. 4655 / #ccffcc
                        2: 4656 .. 4724 / #ccffcc / signal sequence
                     Cleavage site after base 4655"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4725..4829)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    4942..5076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(5501..5521)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     intron          5651..5716
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /note="color: #------"
     CDS             complement(6136..6930)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7291..7620)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: LEFT"
     rep_origin      7305..7440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
ORIGIN
        1 ctcgaggagc ttggcccatt gcatacgttg tatccatatc ataatatgta cagagtaatt
       61 catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact
      121 aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac
      181 tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg
      241 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
      301 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
      361 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
      421 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
      481 tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga
      541 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
      601 ttcgcctcgt gcttgagttg aggcctggct tgggcgctgg ggccgccgcg tgcgaatctg
      661 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
      721 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
      781 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
      841 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
      901 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
      961 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     1021 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     1081 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     1141 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     1201 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     1261 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     1321 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     1381 gtcgtgagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     1441 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt caccatgtct
     1501 agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg agtcggtatc
     1561 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     1621 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     1681 catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     1741 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     1801 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     1861 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     1921 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     1981 accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc
     2041 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     2101 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     2161 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     2221 cttgacatgc tccccgggta actaagtaag gatccagaca tgataagata cattgatgag
     2281 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2341 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     2401 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2461 ctctacaaat gtggtatggc tgattatgat cctgcaagcc tcgtcgtcct ggccggacca
     2521 cgctatctgt gcaaggtccc cggccccgga cgcgcgctcc atgagcagag cgcccgccgc
     2581 cgaggcgaag actcgggcgg cgccctgccc gtcccaccag gtcaacaggc ggtaaccggc
     2641 ctcttcatcg ggaatgcgcg cgaccttcag catcgccggc atgtccccct ggcggacggg
     2701 aagtatccag ctcgaccaag cttggcgaga ttttcaggag ctaaggaagc taaaatggag
     2761 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     2821 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct gcattaatga
     2881 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2941 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3001 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3061 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3121 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3181 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3241 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3301 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3361 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3421 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3481 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3541 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3601 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3661 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3721 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3781 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3841 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3901 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3961 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4021 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4081 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4141 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4201 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4261 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4321 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4381 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4441 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     4501 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4561 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4621 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4681 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4741 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4801 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4861 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4921 cgtcttcact cgaggtcgag ggatccagac atgataagat acattgatga gtttggacaa
     4981 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5041 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     5101 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     5161 tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat tccttattaa
     5221 cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta ttaatagcag
     5281 acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa ctgttatgcc
     5341 tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt gcagcttttt
     5401 cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca gcatgactca
     5461 aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc ttcttttttg
     5521 gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc atttcttctg
     5581 agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca tcagttccat
     5641 aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat tatacactta
     5701 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
     5761 caccacagaa gtaaggttcc ttcacaaaga tccggaccaa agcggccatc gtgcctcccc
     5821 actcctgcag ttcgggggca tggatgcgcg gatagccgct gctggtttcc tggatgccga
     5881 cggatttgca ctgccggtag aactccgcga ggtcgtccag cctcaggcag cagctgaacc
     5941 aactcgcgag gggatcgagc ccggggtggg cgaagaactc cagcatgaga tccccgcgct
     6001 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     6061 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     6121 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     6181 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     6241 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     6301 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     6361 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     6421 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     6481 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     6541 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     6601 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     6661 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     6721 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     6781 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     6841 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     6901 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     6961 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     7021 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     7081 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     7141 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     7201 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     7261 tgagtgcttg cggcagcgtg aagctagctt tttgcaaaag cctaggcctc caaaaaagcc
     7321 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     7381 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     7441 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     7501 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     7561 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     7621 attccacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7681 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7741 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     7801 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcacct
//