| 出品公司: | -- |
|---|---|
| 载体名称: | pECMV-3×FLAG-N,pCDNA3.1-3×FLAG-N |
| 质粒类型: | 哺乳动物细胞载体 |
| 终止子: | SV40 poly(A) signal |
| 复制子: | pUC |
| 启动子: | CMV |
| 载体大小: | 5508 bp |
| 5' 测序引物及序列: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
| 3' 测序引物及序列: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
| 载体标签: | N-3×FLAG |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | G418 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 293T等哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pECMV-3×FLAG-N载体质粒基本信息
pECMV-3×FLAG-N质粒图谱载体图谱和pECMV-3×FLAG-N载体序列质粒序列多克隆位点信息
pECMV-3×FLAG-N质粒载体简介
pECMV-3×FLAG-N质粒序列载体序列
LOCUS pECMV-3×FLAG-N 5502 bp ds-DNA circular SYN 30-JUN-2017
DEFINITION synthetic circular DNA
KEYWORDS pECMV-3×FLAG-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5502)
TITLE Direct Submission
JOURNAL Exported Friday, June 30, 2017 from MiaoLingPlasmid
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..5502
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 922..987
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/note="3xFLAG"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
misc_feature 985..1076
/note="MCS"
polyA_signal 1102..1326
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1372..1800
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1814..2143
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1994..2129
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2210..3004
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3178..3299
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(3348..3364)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3372..3388
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3396..3426)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3441..3462
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3750..4335)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4506..5366)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5367..5471)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac
961 atcgactaca aggatgacga tgacaagctt ggtaccgagc tcggatccac tagtccagtg
1021 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
1081 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
1141 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
1201 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
1261 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
1321 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
1381 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
1441 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
1501 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
1561 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
1621 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
1681 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
1741 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
1801 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
1861 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
1921 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
1981 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
2041 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
2101 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
2161 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
2221 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
2281 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
2341 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
2401 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
2461 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
2521 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
2581 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
2641 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
2701 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
2761 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
2821 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
2881 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
2941 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
3001 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
3061 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
3121 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
3181 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
3241 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
3301 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
3361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
3421 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
3481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
3541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
3601 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
3661 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
3721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
3781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
3841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
3901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
3961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
4021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
4081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
4141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
4201 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
4261 ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga
4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
pECMV-3×FLAG-N载体图谱质粒图谱pdf版和pECMV-3×FLAG-N载体序列质粒序列等相关资料下载
pECMV-3×FLAG-N质粒载体应用举例
- 上一篇：pFLAG CMV 5.1
- 下一篇：pEF6/V5-His B
