pX601

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pX601 ;  pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA 或 pX601-CMV-SaCas9(reopt)-3xHA-U6-BsaI(sgRNA)
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: U6
载体大小: 7446 bp
5' 测序引物及序列: CMV fwd:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: BGH rev:TAGAAGGCACAGTCGAGG
载体标签: NLS (N ter);NLS、3xHA (C ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞等
备注: SaCas9 is form Staphylococcus aureus。
产品目录号: #61591
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pX601载体图谱



载体简介

载体序列

LOCUS       pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA	7447 bp 	DNA	SYN
DEFINITION  pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA"
                     /mol_type="other DNA"
     promoter        155..731
                     /label="CMV_immearly_promoter"
     misc_feature    234..521
                     /label="CAG_enhancer"
     misc_feature    688..708
                     /label="CMV_fwd_primer"
     CDS             693..4106
                     /label="ORF frame 3"
                     /translation="MGGRRVRWEVYISRALWLTTGATMAPKKKRKVGIHGVPAAKRNY
                     ILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRHR
                     IQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNV
                     NEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKE
                     AKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHC
                     TYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPT
                     LKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAK
                     ILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTN
                     DNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYG
                     LPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEKIKLH
                     DMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQEENSKKGN
                     RTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFIN
                     RNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH
                     AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQ
                     IKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLK
                     KLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGP
                     VIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDV
                     IKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNR
                     IEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKGKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*"
     CDS             complement(744..3479)
                     /label="ORF frame 3"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    768..788
                     /label="SV40 NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    768..791
                     /label="SV40 NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    774..788
                     /label="NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     CDS             complement(1973..2431)
                     /label="ORF frame 1"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    4023..4049
                     /label="HA"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    complement(4131..4148)
                     /label="BGH_rev_primer"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     terminator      4134..4337
                     /label="bGH_PA_terminator"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     promoter        4361..4593
                     /label="hU6_promoter"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    4523..4542
                     /label="LKO1_5_primer"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     rep_origin      4942..5248
                     /label="f1_origin"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    5516..5538
                     /label="pGEX_3_primer"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     promoter        5697..5725
                     /label="AmpR_promoter"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     gene            5767..6627
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     CDS             5767..6627
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6782..7401
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
   61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
  121 aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat
  181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
  241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
  301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
  361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
  421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
  481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
  541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
  601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
  661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
  721 tctatataag cagagctctc tggctaacta ccggtgccac cATGGCCCCA AAGAAGAAGC
  781 GGAAGGTCGG TATCCACGGA GTCCCAGCAG CCAAGCGGAA CTACATCCTG GGCCTGGACA
  841 TCGGCATCAC CAGCGTGGGC TACGGCATCA TCGACTACGA GACACGGGAC GTGATCGATG
  901 CCGGCGTGCG GCTGTTCAAA GAGGCCAACG TGGAAAACAA CGAGGGCAGG CGGAGCAAGA
  961 GAGGCGCCAG AAGGCTGAAG CGGCGGAGGC GGCATAGAAT CCAGAGAGTG AAGAAGCTGC
 1021 TGTTCGACTA CAACCTGCTG ACCGACCACA GCGAGCTGAG CGGCATCAAC CCCTACGAGG
 1081 CCAGAGTGAA GGGCCTGAGC CAGAAGCTGA GCGAGGAAGA GTTCTCTGCC GCCCTGCTGC
 1141 ACCTGGCCAA GAGAAGAGGC GTGCACAACG TGAACGAGGT GGAAGAGGAC ACCGGCAACG
 1201 AGCTGTCCAC CAAAGAGCAG ATCAGCCGGA ACAGCAAGGC CCTGGAAGAG AAATACGTGG
 1261 CCGAACTGCA GCTGGAACGG CTGAAGAAAG ACGGCGAAGT GCGGGGCAGC ATCAACAGAT
 1321 TCAAGACCAG CGACTACGTG AAAGAAGCCA AACAGCTGCT GAAGGTGCAG AAGGCCTACC
 1381 ACCAGCTGGA CCAGAGCTTC ATCGACACCT ACATCGACCT GCTGGAAACC CGGCGGACCT
 1441 ACTATGAGGG ACCTGGCGAG GGCAGCCCCT TCGGCTGGAA GGACATCAAA GAATGGTACG
 1501 AGATGCTGAT GGGCCACTGC ACCTACTTCC CCGAGGAACT GCGGAGCGTG AAGTACGCCT
 1561 ACAACGCCGA CCTGTACAAC GCCCTGAACG ACCTGAACAA TCTCGTGATC ACCAGGGACG
 1621 AGAACGAGAA GCTGGAATAT TACGAGAAGT TCCAGATCAT CGAGAACGTG TTCAAGCAGA
 1681 AGAAGAAGCC CACCCTGAAG CAGATCGCCA AAGAAATCCT CGTGAACGAA GAGGATATTA
 1741 AGGGCTACAG AGTGACCAGC ACCGGCAAGC CCGAGTTCAC CAACCTGAAG GTGTACCACG
 1801 ACATCAAGGA CATTACCGCC CGGAAAGAGA TTATTGAGAA CGCCGAGCTG CTGGATCAGA
 1861 TTGCCAAGAT CCTGACCATC TACCAGAGCA GCGAGGACAT CCAGGAAGAA CTGACCAATC
 1921 TGAACTCCGA GCTGACCCAG GAAGAGATCG AGCAGATCTC TAATCTGAAG GGCTATACCG
 1981 GCACCCACAA CCTGAGCCTG AAGGCCATCA ACCTGATCCT GGACGAGCTG TGGCACACCA
 2041 ACGACAACCA GATCGCTATC TTCAACCGGC TGAAGCTGGT GCCCAAGAAG GTGGACCTGT
 2101 CCCAGCAGAA AGAGATCCCC ACCACCCTGG TGGACGACTT CATCCTGAGC CCCGTCGTGA
 2161 AGAGAAGCTT CATCCAGAGC ATCAAAGTGA TCAACGCCAT CATCAAGAAG TACGGCCTGC
 2221 CCAACGACAT CATTATCGAG CTGGCCCGCG AGAAGAACTC CAAGGACGCC CAGAAAATGA
 2281 TCAACGAGAT GCAGAAGCGG AACCGGCAGA CCAACGAGCG GATCGAGGAA ATCATCCGGA
 2341 CCACCGGCAA AGAGAACGCC AAGTACCTGA TCGAGAAGAT CAAGCTGCAC GACATGCAGG
 2401 AAGGCAAGTG CCTGTACAGC CTGGAAGCCA TCCCTCTGGA AGATCTGCTG AACAACCCCT
 2461 TCAACTATGA GGTGGACCAC ATCATCCCCA GAAGCGTGTC CTTCGACAAC AGCTTCAACA
 2521 ACAAGGTGCT CGTGAAGCAG GAAGAAAACA GCAAGAAGGG CAACCGGACC CCATTCCAGT
 2581 ACCTGAGCAG CAGCGACAGC AAGATCAGCT ACGAAACCTT CAAGAAGCAC ATCCTGAATC
 2641 TGGCCAAGGG CAAGGGCAGA ATCAGCAAGA CCAAGAAAGA GTATCTGCTG GAAGAACGGG
 2701 ACATCAACAG GTTCTCCGTG CAGAAAGACT TCATCAACCG GAACCTGGTG GATACCAGAT
 2761 ACGCCACCAG AGGCCTGATG AACCTGCTGC GGAGCTACTT CAGAGTGAAC AACCTGGACG
 2821 TGAAAGTGAA GTCCATCAAT GGCGGCTTCA CCAGCTTTCT GCGGCGGAAG TGGAAGTTTA
 2881 AGAAAGAGCG GAACAAGGGG TACAAGCACC ACGCCGAGGA CGCCCTGATC ATTGCCAACG
 2941 CCGATTTCAT CTTCAAAGAG TGGAAGAAAC TGGACAAGGC CAAAAAAGTG ATGGAAAACC
 3001 AGATGTTCGA GGAAAAGCAG GCCGAGAGCA TGCCCGAGAT CGAAACCGAG CAGGAGTACA
 3061 AAGAGATCTT CATCACCCCC CACCAGATCA AGCACATTAA GGACTTCAAG GACTACAAGT
 3121 ACAGCCACCG GGTGGACAAG AAGCCTAATA GAGAGCTGAT TAACGACACC CTGTACTCCA
 3181 CCCGGAAGGA CGACAAGGGC AACACCCTGA TCGTGAACAA TCTGAACGGC CTGTACGACA
 3241 AGGACAATGA CAAGCTGAAA AAGCTGATCA ACAAGAGCCC CGAAAAGCTG CTGATGTACC
 3301 ACCACGACCC CCAGACCTAC CAGAAACTGA AGCTGATTAT GGAACAGTAC GGCGACGAGA
 3361 AGAATCCCCT GTACAAGTAC TACGAGGAAA CCGGGAACTA CCTGACCAAG TACTCCAAAA
 3421 AGGACAACGG CCCCGTGATC AAGAAGATTA AGTATTACGG CAACAAACTG AACGCCCATC
 3481 TGGACATCAC CGACGACTAC CCCAACAGCA GAAACAAGGT CGTGAAGCTG TCCCTGAAGC
 3541 CCTACAGATT CGACGTGTAC CTGGACAATG GCGTGTACAA GTTCGTGACC GTGAAGAATC
 3601 TGGATGTGAT CAAAAAAGAA AACTACTACG AAGTGAATAG CAAGTGCTAT GAGGAAGCTA
 3661 AGAAGCTGAA GAAGATCAGC AACCAGGCCG AGTTTATCGC CTCCTTCTAC AACAACGATC
 3721 TGATCAAGAT CAACGGCGAG CTGTATAGAG TGATCGGCGT GAACAACGAC CTGCTGAACC
 3781 GGATCGAAGT GAACATGATC GACATCACCT ACCGCGAGTA CCTGGAAAAC ATGAACGACA
 3841 AGAGGCCCCC CAGGATCATT AAGACAATCG CCTCCAAGAC CCAGAGCATT AAGAAGTACA
 3901 GCACAGACAT TCTGGGCAAC CTGTATGAAG TGAAATCTAA GAAGCACCCT CAGATCATCA
 3961 AAAAGGGCAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG AAAAAGggat
 4021 cctacccata cgatgttcca gattacgctt acccatacga tgttccagat tacgcttacc
 4081 catacgatgt tccagattac gcttaagaat tcctagagct cgctgatcag cctcgactgt
 4141 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
 4201 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
 4261 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
 4321 agagaatagc aggcatgctg gggaggtacc tgagggccta tttcccatga ttccttcata
 4381 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
 4441 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
 4501 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga
 4561 tttcttggct ttatatatct tGTGGAAAGG ACGAAACACC gGAGACCacg gcaGGTCTCa
 4621 gttttagtac tctggaaaca gaatctacta aaacaaggca aaatgccgtg tttatctcgt
 4681 caacttgttg gcgagatttt tgcggccgca ggaaccccta gtgatggagt tggccactcc
 4741 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
 4801 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
 4861 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
 4921 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
 4981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
 5041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
 5101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
 5161 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
 5221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
 5281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
 5341 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
 5401 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
 5461 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
 5521 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
 5581 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
 5641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
 5701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
 5761 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
 5821 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
 5881 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
 5941 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
 6001 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
 6061 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
 6121 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
 6181 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
 6241 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
 6301 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
 6361 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
 6421 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
 6481 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
 6541 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
 6601 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
 6661 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
 6721 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
 6781 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
 6841 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
 6901 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
 6961 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
 7021 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
 7081 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
 7141 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
 7201 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
 7261 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
 7321 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
 7381 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
 7441 cacatgt
//

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