pX600

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pX600 ; pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 7101 bp
5' 测序引物及序列: CMV fwd:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: BGH rev:TAGAAGGCACAGTCGAGG
载体标签: NLS (N ter);NLS、3xHA(C ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞等
备注: A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.
产品目录号: #61592
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pX600载体图谱



载体简介

载体序列

LOCUS       pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA	7101 bp 	DNA	SYN
DEFINITION  pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA"
                     /mol_type="other DNA"
     rep_origin      complement(47..666)
                     /label="pBR322_origin"
     gene            complement(821..1681)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(821..1681)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(1723..1751)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(1910..1932)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      complement(2200..2506)
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        2759..3335
                     /label="CMV_immearly_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    2838..3125
                     /label="CAG_enhancer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    3292..3312
                     /label="CMV_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             3297..6710
                     /label="ORF frame 3"
                     /translation="MGGRRVRWEVYISRALWLTTGATMAPKKKRKVGIHGVPAAKRNY
                     ILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRHR
                     IQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNV
                     NEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKE
                     AKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHC
                     TYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPT
                     LKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAK
                     ILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTN
                     DNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYG
                     LPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEKIKLH
                     DMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQEENSKKGN
                     RTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFIN
                     RNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH
                     AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQ
                     IKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLK
                     KLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGP
                     VIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDV
                     IKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNR
                     IEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKGKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*"
     CDS             complement(3348..6083)
                     /label="ORF frame 2"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    3372..3392
                     /label="SV40 NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    3372..3395
                     /label="SV40 NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     misc_feature    3378..3392
                     /label="NLS"
                     /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV
                     LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV
                     QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL
                     VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH
                     FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL
                     ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAVFFLLHEHLVVEAVVEGHASGDDV
                     VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP
                     VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH
                     QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD
                     LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV
                     GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD
                     HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP
                     PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG
                     HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH
                     SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ
                     PHAGIDHVPCLVVDDAVAHAGDADVQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*"
     CDS             complement(4577..5035)
                     /label="ORF frame 3"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    6627..6653
                     /label="HA"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     misc_feature    complement(6735..6752)
                     /label="BGH_rev_primer"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
     terminator      6738..6941
                     /label="bGH_PA_terminator"
                     /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF
                     RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR
                     VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*"
ORIGIN
    1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
   61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
  121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
  181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
  241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
  301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
  361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
  421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
  481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
  541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
  601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
  661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
  721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
  781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
  841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
  901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
  961 ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
 1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
 1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
 1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
 1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
 1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
 1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
 1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
 1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
 1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
 1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
 1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
 1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
 1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
 1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
 1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
 1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
 1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
 2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg
 2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
 2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga
 2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
 2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag
 2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
 2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
 2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
 2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa
 2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
 2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg
 2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcctcg aggcgttgac
 2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
 2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
 2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
 2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
 3001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
 3061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
 3121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
 3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
 3241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
 3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actaccggtg
 3361 ccaccATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCAAGC
 3421 GGAACTACAT CCTGGGCCTG GACATCGGCA TCACCAGCGT GGGCTACGGC ATCATCGACT
 3481 ACGAGACACG GGACGTGATC GATGCCGGCG TGCGGCTGTT CAAAGAGGCC AACGTGGAAA
 3541 ACAACGAGGG CAGGCGGAGC AAGAGAGGCG CCAGAAGGCT GAAGCGGCGG AGGCGGCATA
 3601 GAATCCAGAG AGTGAAGAAG CTGCTGTTCG ACTACAACCT GCTGACCGAC CACAGCGAGC
 3661 TGAGCGGCAT CAACCCCTAC GAGGCCAGAG TGAAGGGCCT GAGCCAGAAG CTGAGCGAGG
 3721 AAGAGTTCTC TGCCGCCCTG CTGCACCTGG CCAAGAGAAG AGGCGTGCAC AACGTGAACG
 3781 AGGTGGAAGA GGACACCGGC AACGAGCTGT CCACCAAAGA GCAGATCAGC CGGAACAGCA
 3841 AGGCCCTGGA AGAGAAATAC GTGGCCGAAC TGCAGCTGGA ACGGCTGAAG AAAGACGGCG
 3901 AAGTGCGGGG CAGCATCAAC AGATTCAAGA CCAGCGACTA CGTGAAAGAA GCCAAACAGC
 3961 TGCTGAAGGT GCAGAAGGCC TACCACCAGC TGGACCAGAG CTTCATCGAC ACCTACATCG
 4021 ACCTGCTGGA AACCCGGCGG ACCTACTATG AGGGACCTGG CGAGGGCAGC CCCTTCGGCT
 4081 GGAAGGACAT CAAAGAATGG TACGAGATGC TGATGGGCCA CTGCACCTAC TTCCCCGAGG
 4141 AACTGCGGAG CGTGAAGTAC GCCTACAACG CCGACCTGTA CAACGCCCTG AACGACCTGA
 4201 ACAATCTCGT GATCACCAGG GACGAGAACG AGAAGCTGGA ATATTACGAG AAGTTCCAGA
 4261 TCATCGAGAA CGTGTTCAAG CAGAAGAAGA AGCCCACCCT GAAGCAGATC GCCAAAGAAA
 4321 TCCTCGTGAA CGAAGAGGAT ATTAAGGGCT ACAGAGTGAC CAGCACCGGC AAGCCCGAGT
 4381 TCACCAACCT GAAGGTGTAC CACGACATCA AGGACATTAC CGCCCGGAAA GAGATTATTG
 4441 AGAACGCCGA GCTGCTGGAT CAGATTGCCA AGATCCTGAC CATCTACCAG AGCAGCGAGG
 4501 ACATCCAGGA AGAACTGACC AATCTGAACT CCGAGCTGAC CCAGGAAGAG ATCGAGCAGA
 4561 TCTCTAATCT GAAGGGCTAT ACCGGCACCC ACAACCTGAG CCTGAAGGCC ATCAACCTGA
 4621 TCCTGGACGA GCTGTGGCAC ACCAACGACA ACCAGATCGC TATCTTCAAC CGGCTGAAGC
 4681 TGGTGCCCAA GAAGGTGGAC CTGTCCCAGC AGAAAGAGAT CCCCACCACC CTGGTGGACG
 4741 ACTTCATCCT GAGCCCCGTC GTGAAGAGAA GCTTCATCCA GAGCATCAAA GTGATCAACG
 4801 CCATCATCAA GAAGTACGGC CTGCCCAACG ACATCATTAT CGAGCTGGCC CGCGAGAAGA
 4861 ACTCCAAGGA CGCCCAGAAA ATGATCAACG AGATGCAGAA GCGGAACCGG CAGACCAACG
 4921 AGCGGATCGA GGAAATCATC CGGACCACCG GCAAAGAGAA CGCCAAGTAC CTGATCGAGA
 4981 AGATCAAGCT GCACGACATG CAGGAAGGCA AGTGCCTGTA CAGCCTGGAA GCCATCCCTC
 5041 TGGAAGATCT GCTGAACAAC CCCTTCAACT ATGAGGTGGA CCACATCATC CCCAGAAGCG
 5101 TGTCCTTCGA CAACAGCTTC AACAACAAGG TGCTCGTGAA GCAGGAAGAA AACAGCAAGA
 5161 AGGGCAACCG GACCCCATTC CAGTACCTGA GCAGCAGCGA CAGCAAGATC AGCTACGAAA
 5221 CCTTCAAGAA GCACATCCTG AATCTGGCCA AGGGCAAGGG CAGAATCAGC AAGACCAAGA
 5281 AAGAGTATCT GCTGGAAGAA CGGGACATCA ACAGGTTCTC CGTGCAGAAA GACTTCATCA
 5341 ACCGGAACCT GGTGGATACC AGATACGCCA CCAGAGGCCT GATGAACCTG CTGCGGAGCT
 5401 ACTTCAGAGT GAACAACCTG GACGTGAAAG TGAAGTCCAT CAATGGCGGC TTCACCAGCT
 5461 TTCTGCGGCG GAAGTGGAAG TTTAAGAAAG AGCGGAACAA GGGGTACAAG CACCACGCCG
 5521 AGGACGCCCT GATCATTGCC AACGCCGATT TCATCTTCAA AGAGTGGAAG AAACTGGACA
 5581 AGGCCAAAAA AGTGATGGAA AACCAGATGT TCGAGGAAAA GCAGGCCGAG AGCATGCCCG
 5641 AGATCGAAAC CGAGCAGGAG TACAAAGAGA TCTTCATCAC CCCCCACCAG ATCAAGCACA
 5701 TTAAGGACTT CAAGGACTAC AAGTACAGCC ACCGGGTGGA CAAGAAGCCT AATAGAGAGC
 5761 TGATTAACGA CACCCTGTAC TCCACCCGGA AGGACGACAA GGGCAACACC CTGATCGTGA
 5821 ACAATCTGAA CGGCCTGTAC GACAAGGACA ATGACAAGCT GAAAAAGCTG ATCAACAAGA
 5881 GCCCCGAAAA GCTGCTGATG TACCACCACG ACCCCCAGAC CTACCAGAAA CTGAAGCTGA
 5941 TTATGGAACA GTACGGCGAC GAGAAGAATC CCCTGTACAA GTACTACGAG GAAACCGGGA
 6001 ACTACCTGAC CAAGTACTCC AAAAAGGACA ACGGCCCCGT GATCAAGAAG ATTAAGTATT
 6061 ACGGCAACAA ACTGAACGCC CATCTGGACA TCACCGACGA CTACCCCAAC AGCAGAAACA
 6121 AGGTCGTGAA GCTGTCCCTG AAGCCCTACA GATTCGACGT GTACCTGGAC AATGGCGTGT
 6181 ACAAGTTCGT GACCGTGAAG AATCTGGATG TGATCAAAAA AGAAAACTAC TACGAAGTGA
 6241 ATAGCAAGTG CTATGAGGAA GCTAAGAAGC TGAAGAAGAT CAGCAACCAG GCCGAGTTTA
 6301 TCGCCTCCTT CTACAACAAC GATCTGATCA AGATCAACGG CGAGCTGTAT AGAGTGATCG
 6361 GCGTGAACAA CGACCTGCTG AACCGGATCG AAGTGAACAT GATCGACATC ACCTACCGCG
 6421 AGTACCTGGA AAACATGAAC GACAAGAGGC CCCCCAGGAT CATTAAGACA ATCGCCTCCA
 6481 AGACCCAGAG CATTAAGAAG TACAGCACAG ACATTCTGGG CAACCTGTAT GAAGTGAAAT
 6541 CTAAGAAGCA CCCTCAGATC ATCAAAAAGG GCAAAAGGCC GGCGGCCACG AAAAAGGCCG
 6601 GCCAGGCAAA AAAGAAAAAG ggatccTACC CATACGATGT TCCAGATTAC GCTTACCCAT
 6661 ACGATGTTCC AGATTACGCT TACCCATACG ATGTTCCAGA TTACGCTTAA GAAttcctag
 6721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
 6781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
 6841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
 6901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagC TAGAggccgc
 6961 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
 7021 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
 7081 gagcgcgcag ctgcctgcag g
//

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