PX856

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: PX856
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
插入位点: Age1,EcoR1
启动子: CBh
载体大小: 7351bp
5' 测序引物及序列: ctggagcacctgcctgaaatcact
3' 测序引物及序列: GGCTGATCAGCGAGCTCTAG
载体标签: NES、PTK2、FKBP12(N ter);NLS、VP64(C ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞等
备注: A portion of this plasmid was derived from a plasmid made by SpCas9 piece was PCR amplified of PX429;Mutation Asparagine 863 to Alanine (N863A)
产品目录号: #62888
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

PX856载体图谱



载体简介

载体序列

LOCUS       PX856	7351 bp 	DNA	SYN
DEFINITION  PX856
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="PX856"
                     /mol_type="other DNA"
     promoter        10..242
                     /label="hU6_promoter"
     misc_feature    172..191
                     /label="LKO1_5_primer"
     misc_feature    444..713
                     /label="CAG_enhancer"
     CDS             complement(1230..2582)
                     /label="ORF frame 3"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLRATATRATATRATATLQLQQLHVEHQGGV
                     RRDDAGVAGGAVGVVGADGQLGSLAHAHLGHALLPASDHLLLAQHELEGLVSVSAAVE
                     LLAVLQHAGVVHHAGLASLGEGSAVAGADGLHLHAGCWDSVDTDLPLLLWGHGGTGPT
                     *"
     CDS             1251..4367
                     /label="ORF frame 3"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     misc_feature    1257..1277
                     /label="SV40 NLS"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     misc_feature    1257..1280
                     /label="SV40 NLS"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     misc_feature    1263..1277
                     /label="NLS"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     misc_feature    complement(4392..4409)
                     /label="BGH_rev_primer"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     terminator      4395..4598
                     /label="bGH_PA_terminator"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     rep_origin      4846..5152
                     /label="f1_origin"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     misc_feature    5420..5442
                     /label="pGEX_3_primer"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     promoter        5601..5629
                     /label="AmpR_promoter"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     gene            5671..6531
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MAPKKKRKVGIHGVPAAGVQVETISPGDGRTFPKRGQTCVVHYT
                     GMLEDGKKFDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGAT
                     GHPGIIPPHATLVFDVELLKLEGGGGSGGGGSGGGGSKPAFLSGEQKKAIVDLLFKTN
                     RKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENED
                     ILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRD
                     KQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGS
                     PAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGI
                     KELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSF
                     LKDDSIDNKVLTRSDKARGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAE
                     RGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
                     VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRK
                     MIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGR
                     DFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDS
                     PTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
                     IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
                     EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENII
                     HLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
                     SPKKKRKVEASGRADALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDD
                     FDLDMLIN*"
     CDS             5671..6531
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6686..7305
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
   61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
  121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
  181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
  241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
  301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
  361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
  421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
  481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
  541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
  601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
  661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
  721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
  781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
  841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
  901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
  961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
 1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
 1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
 1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
 1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGGCCCCAA
 1261 AGAAGAAGCG GAAGGTCGGT ATCCACGGAG TCCCAGCAGC CGGCGTGCAG GTGGAGACCA
 1321 TCAGCCCCGG CGACGGCAGA ACCTTCCCCA AGAGAGGCCA GACCTGCGTG GTGCACTACA
 1381 CCGGCATGCT GGAGGACGGC AAGAAGTTCG ACAGCAGCAG AGACAGAAAC AAGCCCTTCA
 1441 AGTTCATGCT GGGCAAGCAG GAGGTGATCA GAGGCTGGGA GGAGGGCGTG GCCCAGATGA
 1501 GCGTGGGCCA GAGAGCCAAG CTGACCATCA GCCCCGACTA CGCCTACGGC GCCACCGGCC
 1561 ACCCCGGCAT CATCCCTCCT CACGCCACCC TGGTGTTCGA CGTGGAGCTG CTGAAGCTGG
 1621 AGGGTGGCGG TGGCtcgGGT GGCGGTGGCt cgGGTGGCGG TGGCtcgAAG CCCGCCTTCC
 1681 TGAGCGGCGA GCAGAAAAAG GCCATCGTGG ACCTGCTGTT CAAGACCAAC CGGAAAGTGA
 1741 CCGTGAAGCA GCTGAAAGAG GACTACTTCA AGAAAATCGA GTGCTTCGAC TCCGTGGAAA
 1801 TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCAC ATACCACGAT CTGCTGAAAA
 1861 TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG GAAGATATCG
 1921 TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG AAAACCTATG
 1981 CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC ACCGGCTGGG
 2041 GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC AAGACAATCC
 2101 TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG ATCCACGACG
 2161 ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG GGCGATAGCC
 2221 TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC ATCCTGCAGA
 2281 CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC GAGAACATCG
 2341 TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC AGCCGCGAGA
 2401 GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG AAAGAACACC
 2461 CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG CAGAATGGGC
 2521 GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC GATGTGGACC
 2581 ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG CTGACCAGAA
 2641 GCGACAAGgc CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG AAGAAGATGA
 2701 AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG TTCGACAATC
 2761 TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC ATCAAGAGAC
 2821 AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC TCCCGGATGA
 2881 ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC ACCCTGAAGT
 2941 CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC GAGATCAACA
 3001 ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC CTGATCAAAA
 3061 AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC GACGTGCGGA
 3121 AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC TTCTTCTACA
 3181 GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG ATCCGGAAGC
 3241 GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG GGCCGGGATT
 3301 TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA AAGACCGAGG
 3361 TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC GATAAGCTGA
 3421 TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC CCCACCGTGG
 3481 CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA CTGAAGAGTG
 3541 TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG AATCCCATCG
 3601 ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC AAGCTGCCTA
 3661 AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT GCCGGCGAAC
 3721 TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG TACCTGGCCA
 3781 GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG CTGTTTGTGG
 3841 AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC TCCAAGAGAG
 3901 TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG CACCGGGATA
 3961 AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC AATCTGGGAG
 4021 CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC ACCAGCACCA
 4081 AAGAGGTGCT GGACGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC GAGACACGGA
 4141 TCGACCTGTC TCAGCTGGGA GGCGACAGCC CCAAGAAGAA GAGAAAGGTG GAGGCCAGCG
 4201 GACGGGCTGA CGCATTGGAC GATTTTGATC TGGATATGCT GGGAAGTGAC GCCCTCGATG
 4261 ATTTTGACCT TGACATGCTT GGTTCGGATG CCCTTGATGA CTTTGACCTC GACATGCTCG
 4321 GCAGTGACGC CCTTGATGAT TTCGACCTGG ACATGCTGAT TAACtaagaa ttcCTAGAGC
 4381 TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC
 4441 CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA
 4501 AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA
 4561 CAGCAAGGGG GAGGATTGGG AAGAgAATAG CAGGCATGCT GGGGAgcggc cgcaggaacc
 4621 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
 4681 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
 4741 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
 4801 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
 4861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
 4921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
 4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
 5041 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
 5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
 5161 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
 5221 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
 5281 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
 5341 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
 5401 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
 5461 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
 5521 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
 5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
 5641 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
 5701 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
 5761 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
 5821 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
 5881 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
 5941 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
 6001 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
 6061 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
 6121 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
 6181 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
 6241 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
 6301 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
 6361 gataaatctg gagccggtga gcgtggaagc cgcggtatca ttgcagcact ggggccagat
 6421 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
 6481 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
 6541 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
 6601 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
 6661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
 6721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
 6781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
 6841 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
 6901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
 6961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
 7021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
 7081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
 7141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
 7201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
 7261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
 7321 ctggcctttt gctggccttt tgctcacatg t
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng