PX853

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX853
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
插入位点: Age1,EcoR1
启动子: CBh
载体大小: 6295 bp
5' 测序引物及序列: ctggagcacctgcctgaaatcact
3' 测序引物及序列: GGGCTGATCAGCGAGCTCTAG
载体标签: NES、PTK2(N ter);FRB(C ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞等
备注: N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5)
产品目录号: #62885
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

PX853载体图谱



载体简介

载体序列

LOCUS       PX853	6295 bp 	DNA	SYN
DEFINITION  PX853
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="PX853"
                     /mol_type="other DNA"
     promoter        10..242
                     /label="hU6_promoter"
     misc_feature    172..191
                     /label="LKO1_5_primer"
     misc_feature    444..713
                     /label="CAG_enhancer"
     CDS             1251..3311
                     /label="ORF frame 3"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     misc_feature    complement(3336..3353)
                     /label="BGH_rev_primer"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     terminator      3339..3542
                     /label="bGH_PA_terminator"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     rep_origin      3790..4096
                     /label="f1_origin"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     misc_feature    4364..4386
                     /label="pGEX_3_primer"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     promoter        4545..4573
                     /label="AmpR_promoter"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     gene            4615..5475
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLDLASLILDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIEGGGGSGGGGSGGGGSEMWHEGLEEASRLYFGERNVKGMFEVLE
                     PLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRR
                     ISKQ*"
     CDS             4615..5475
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5630..6249
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
   61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
  121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
  181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
  241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
  301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
  361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
  421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
  481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
  541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
  601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
  661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
  721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
  781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
  841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
  901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
  961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
 1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
 1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
 1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
 1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGCTAGATT
 1261 TAGCTAGCTT AATCCTCGAC AAGAAGTACA GCATCGGCCT GGACATCGGC ACCAACTCTG
 1321 TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG
 1381 GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG
 1441 GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA
 1501 AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA
 1561 GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC
 1621 ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT
 1681 ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC
 1741 TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC
 1801 CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT
 1861 TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC
 1921 TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG
 1981 GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT
 2041 TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG
 2101 ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC
 2161 TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC
 2221 CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA
 2281 AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA
 2341 AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA
 2401 TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG
 2461 AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC
 2521 TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA
 2581 ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG
 2641 CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT
 2701 GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA
 2761 CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG
 2821 AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAA
 2881 AGCCCGCCTT CCTGAGCGGC GAGCAGAAAA AGGCCATCGT GGACCTGCTG TTCAAGACCA
 2941 ACCGGAAAGT GACCGTGAAG CAGCTGAAAG AGGACTACTT CAAGAAAATC GAGGGTGGCG
 3001 GTGGCtcgGG TGGCGGTGGC tcgGGTGGCG GTGGCtcgGA GATGTGGCAC GAGGGCCTGG
 3061 AGGAGGCCAG CAGACTGTAC TTCGGCGAGA GAAACGTGAA GGGCATGTTC GAGGTGCTGG
 3121 AGCCCCTGCA CGCCATGATG GAGAGAGGCC CCCAGACCCT GAAGGAGACC AGCTTCAACC
 3181 AGGCCTACGG CAGAGACCTG ATGGAGGCCC AGGAGTGGTG CAGAAAGTAC ATGAAGAGCG
 3241 GCAACGTGAA GGACCTGACC CAGGCCTGGG ACCTGTACTA CCACGTGTTC AGAAGAATCA
 3301 GCAAGCAGta agaattcCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
 3361 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
 3421 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
 3481 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGAgA ATAGCAGGCA
 3541 TGCTGGGGAg cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
 3601 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
 3661 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
 3721 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
 3781 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
 3841 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
 3901 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
 3961 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
 4021 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
 4081 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
 4141 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
 4201 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
 4261 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
 4321 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
 4381 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
 4441 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
 4501 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
 4561 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
 4621 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
 4681 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
 4741 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
 4801 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
 4861 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
 4921 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
 4981 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
 5041 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
 5101 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
 5161 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
 5221 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
 5281 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
 5341 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
 5401 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
 5461 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
 5521 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
 5581 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
 5641 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
 5701 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
 5761 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
 5821 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
 5881 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
 5941 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
 6001 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
 6061 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
 6121 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
 6181 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
 6241 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt

//

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