PX552

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX552 或 pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor)
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: U6
载体大小: 5303bp
5' 测序引物及序列: cgcgtgagggcctatttcc
3' 测序引物及序列: gcaagtgggttttaggaccag
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: EGFP
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞
备注: AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.
产品目录号: #60958
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

PX552载体图谱



载体简介

载体序列

LOCUS       PX552	5303 bp 	DNA	SYN
DEFINITION  PX552
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="PX552"
                     /mol_type="other DNA"
     promoter        165..397
                     /label="hU6_promoter"
     misc_feature    327..346
                     /label="LKO1_5_primer"
     CDS             1017..2000
                     /label="ORF frame 3"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     gene            1020..1733
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     misc_feature    complement(1062..1083)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     misc_feature    1200..1229
                     /label="Y66 (EGFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     misc_feature    1670..1691
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     terminator      2048..2526
                     /label="hGH_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     rep_origin      2798..3104
                     /label="f1_origin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     misc_feature    3372..3394
                     /label="pGEX_3_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     promoter        3553..3581
                     /label="AmpR_promoter"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     gene            3623..4483
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKSGLRSREEEEETDSRMPHLDSPGSSQPRRSFLSRVIR
                     AALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT*"
     CDS             3623..4483
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4638..5257
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
   61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
  121 actccatcac taggggttcc tgcggccgca cgcgtgaggg cctatttccc atgattcctt
  181 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
  241 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
  301 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
  361 tcgatttctt ggctttatat atcttGTGGA AAGGACGAAA CACCggaaga gcgagctctt
  421 ctgttttaga gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa
  481 gtggcaccga gtcggtgcTT TTTTtctaga ctgcagaggg ccctgcgtat gagtgcaagt
  541 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
  601 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
  661 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
  721 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
  781 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
  841 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
  901 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
  961 gtcgtgcctg agagcgcagt cgagaaggta ccggatcctc tagagtcgac gccaccatgg
 1021 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
 1081 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
 1141 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
 1201 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
 1261 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
 1321 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
 1381 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
 1441 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
 1501 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
 1561 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
 1621 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
 1681 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
 1741 tcagatctcg agaggaggag gaggagacag acagcaggat gccccacctc gacagccccg
 1801 gcagctccca gccgagacgc tccttcctct caagggtgat cagggcagcg ctaccgttgc
 1861 agctgcttct gctgctgctg ctgctcctgg cctgcctgct acctgcctct gaagatgact
 1921 acagctgcac ccaggccaac aactttgccc gatccttcta ccccatgctg cggtacacca
 1981 acgggccacc tcccacctag gaattcgata tcaagcttat cgataccgag cgctgctcga
 2041 gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
 2101 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
 2161 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
 2221 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
 2281 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
 2341 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
 2401 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
 2461 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
 2521 gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa cccctagtga
 2581 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
 2641 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
 2701 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
 2761 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
 2821 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
 2881 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
 2941 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
 3001 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
 3061 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
 3121 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
 3181 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
 3241 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
 3301 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
 3361 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
 3421 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
 3481 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
 3541 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
 3601 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
 3661 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
 3721 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
 3781 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
 3841 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
 3901 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
 3961 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
 4021 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
 4081 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
 4141 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
 4201 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
 4261 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
 4321 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
 4381 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
 4441 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
 4501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
 4561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
 4621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
 4681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
 4741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
 4801 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
 4861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
 4921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
 4981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
 5041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
 5101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
 5161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
 5221 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
 5281 ttgctggcct tttgctcaca tgt
//

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