PX377

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX377 或 PX377 Corynebacter diphtheriae Cas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;昆虫细胞载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 10448bp
5' 测序引物及序列: CMV Forward:CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: cctagtcagacaaaatgatgcaa
载体标签: NLS (C ter);HA、NLS (N ter)
载体抗性: 氨苄青霉素
筛选标记: Neomycin
克隆菌株: Stbl3
宿主细胞(系): 昆虫细胞
备注: Corynebacter diphtheriae Cas9
产品目录号: #68314
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 杆状病毒

载体质粒图谱和多克隆位点信息

PX377载体图谱



载体简介

载体序列

LOCUS       PX377 Corynebacter diphtheriae Cas9	10448 bp 	DNA	SYN
DEFINITION  PX377 Corynebacter diphtheriae Cas9
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="PX377 Corynebacter diphtheriae Cas9"
                     /mol_type="other DNA"
     misc_feature    47..1001
                     /label="Ac_5_flank"
     CDS             complement(343..948)
                     /label="ORF frame 3"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1023..1587
                     /label="CMV_immearly_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1090..1377
                     /label="CAG_enhancer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1544..1564
                     /label="CMV_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1615..1633
                     /label="T7_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1633..1656
                     /label="lacO"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1772..1796
                     /label="attB1"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             1822..5139
                     /label="ORF frame 1"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1825..1851
                     /label="HA"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1855..1875
                     /label="SV40 NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     misc_feature    1861..1875
                     /label="NLS"
                     /translation="MYPYDVPDYASPKKKRKVEASMKYHVGIDVGTFSVGLAAIEVDD
                     AGMPIKTLSLVSHIHDSGLDPDEIKSAVTRLASSGIARRTRRLYRRKRRRLQQLDKFI
                     QRQGWPVIELEDYSDPLYPWKVRAELAASYIADEKERGEKLSVALRHIARHRGWRNPY
                     AKVSSLYLPDGPSDAFKAIREEIKRASGQPVPETATVGQMVTLCELGTLKLRGEGGVL
                     SARLQQSDYAREIQEICRMQEIGQELYRKIIDVVFAAESPKGSASSRVGKDPLQPGKN
                     RALKASDAFQRYRIAALIGNLRVRVDGEKRILSVEEKNLVFDHLVNLTPKKEPEWVTI
                     AEILGIDRGQLIGTATMTDDGERAGARPPTHDTNRSIVNSRIAPLVDWWKTASALEQH
                     AMVKALSNAEVDDFDSPEGAKVQAFFADLDDDVHAKLDSLHLPVGRAAYSEDTLVRLT
                     RRMLSDGVDLYTARLQEFGIEPSWTPPTPRIGEPVGNPAVDRVLKTVSRWLESATKTW
                     GAPERVIIEHVREGFVTEKRAREMDGDMRRRAARNAKLFQEMQEKLNVQGKPSRADLW
                     RYQSVQRQNCQCAYCGSPITFSNSEMDHIVPRAGQGSTNTRENLVAVCHRCNQSKGNT
                     PFAIWAKNTSIEGVSVKEAVERTRHWVTDTGMRSTDFKKFTKAVVERFQRATMDEEID
                     ARSMESVAWMANELRSRVAQHFASHGTTVRVYRGSLTAEARRASGISGKLKFFDGVGK
                     SRLDRRHHAIDAAVIAFTSDYVAETLAVRSNLKQSQAHRQEAPQWREFTGKDAEHRAA
                     WRVWCQKMEKLSALLTEDLRDDRVVVMSNVRLRLGNGSAHKETIGKLSKVKLSSQLSV
                     SDIDKASSEALWCALTREPGFDPKEGLPANPERHIRVNGTHVYAGDNIGLFPVSAGSI
                     ALRGGYAELGSSFHHARVYKITSGKKPAFAMLRVYTIDLLPYRNQDLFSVELKPQTMS
                     MRQAEKKLRDALATGNAEYLGWLVVDDELVVDTSKIATDQVKAVEAELGTIRRWRVDG
                     FFSPSKLRLRPLQMSKEGIKKESAPELSKIIDRPGWLPAVNKLFSDGNVTVVRRDSLG
                     RVRLESTAHLPVTWKVQ*"
     CDS             complement(2608..3120)
                     /label="ORF frame 3"
                     /translation="MQAVQLGVDVIVQIGKECLHFCSLGRIKIVDFGVGQCLHHGVLL
                     QCASCFPPVDQWCDPAVHNTSVSIVCRGSGSCSLSIVGHSSCSDQLPAIDAQNFGDGH
                     PFGLFLWSQIHQVVEHQVLLLHAQNPLLPIHPDSQIADQCSNPVSLKSVAGFQCSVLS
                     WLQRIFPHPA*"
     CDS             complement(3529..4065)
                     /label="ORF frame 3"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(5172..5189)
                     /label="Sp6_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    5194..5218
                     /label="attB2"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     terminator      5230..5696
                     /label="hGH_PA_terminator"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     terminator      5702..5737
                     /label="T7_terminator"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     rep_origin      5854..6160
                     /label="f1_origin"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(6274..6294)
                     /label="pBABE_3_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    complement(6280..6495)
                     /label="SV40_enhancer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     promoter        6292..6560
                     /label="SV40_promoter"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     rep_origin      6459..6536
                     /label="SV40_origin"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     misc_feature    6521..6540
                     /label="SV40pro_F_primer"
                     /translation="MVPSIQPGLPHAIKEFQLSTDPRCPSSLCCQAASVHPDCGSMGC
                     EVLSHAAPQLISHPGDGFHASGVDFLIHCCSLKPLDHSLGELLEVSASHAGISDPMSG
                     PFHGLLHTDPFDRSVFCPNSEGCIALRLVTAMAHGDQILPCVGGSLPCSGHDVVHFTI
                     RKGNWGPAIGTLAVLSLH*"
     CDS             6675..7469
                     /label="ORF frame 3"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            6678..7466
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(6984..7520)
                     /label="ORF frame 1"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7646..7765
                     /label="SV40_PA_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    7734..7753
                     /label="EBV_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     terminator      7956..7991
                     /label="T7_terminator"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    complement(8014..8031)
                     /label="Baculovirs_rev_primer"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     misc_feature    8021..8098
                     /label="3_AcPH"
                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA
                     VSPFAAKLFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA
                     VGHARLEPGEQFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ
                     PPHCISHDGYFLGRSKVR*"
     CDS             complement(8128..8745)
                     /label="ORF frame 3"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    8725..8758
                     /label="loxP"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     gene            complement(9526..10386)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MYAIRSYAIAIPKSSTTNLIADVLADTINRRRVAMAKSSSEATS
                     NDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLL
                     QNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIF
                     KFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             complement(9526..10386)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
   61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
  121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
  181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
  241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
  301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
  361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
  421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
  481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
  541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
  601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
  661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
  721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
  781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
  841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
  901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
  961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
 1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
 1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
 1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
 1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
 1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
 1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
 1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
 1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
 1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
 1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
 1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
 1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
 1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
 1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
 1861 aaaaagcgca aggtcgaagc gtccatgaag taccatgtcg gaatcgatgt cggaaccttt
 1921 tctgtggggc tggctgctat tgaagtggat gacgctggaa tgcctattaa gaccctgagt
 1981 ctggtgtcac acattcatga ctcaggactg gatcctgacg agatcaagag cgctgtgacc
 2041 aggctggcaa gctccggaat cgcccggaga acaaggcgcc tgtaccgacg gaagagaagg
 2101 cgcctgcagc agctggataa gttcatccag aggcagggct ggccagtgat cgagctggaa
 2161 gattacagcg accccctgta tccttggaag gtgcgcgccg aactggccgc ttcttatatt
 2221 gctgacgaga aggaacgggg ggagaaactg agtgtggctc tgagacacat cgcaaggcat
 2281 cgcggatgga ggaaccctta cgccaaggtg tctagtctgt atctgccaga tggcccctca
 2341 gacgccttca aggctattag ggaggaaatc aaacgcgcta gcggccagcc tgtgccagag
 2401 actgcaaccg tcgggcagat ggtgaccctg tgcgaactgg gcacactgaa gctgcgagga
 2461 gagggaggag tgctgagtgc acggctgcag cagtcagatt acgcccgcga gatccaggaa
 2521 atttgtcgaa tgcaggagat cggccaggaa ctgtatcgca agatcattga cgtggtgttc
 2581 gcagccgagt ccccaaaggg ctctgcctca agccgggtgg ggaaagatcc tctgcagcca
 2641 ggaaagaaca gagcactgaa agccagcgac gcttttcagc gataccggat tgctgcactg
 2701 atcggcaatc tgagagtcag ggtggatggg gagaagagga ttctgagcgt ggaggagaag
 2761 aacctggtgt tcgaccacct ggtgaatctg actccaaaga aagagcccga atgggtgacc
 2821 atcgccgaaa ttctgggcat cgatcgcggg cagctgatcg gaacagctac tatgaccgac
 2881 gatggagagc gagcaggagc ccgaccccct acacacgata ctaacagaag tattgtgaac
 2941 agccggatcg caccactggt cgactggtgg aaaacagcta gcgcactgga gcagcacgcc
 3001 atggtgaagg cactgtccaa cgccgaagtc gacgattttg attctcccga gggagcaaaa
 3061 gtgcaggcat tctttgccga tctggacgat gacgtccacg ccaagctgga cagcctgcat
 3121 ctgcctgtgg gacgagcagc ttactccgag gacactctgg tcagactgac ccgacggatg
 3181 ctgagtgatg gggtggacct gtataccgcc cggctgcagg agttcggaat tgaacctagc
 3241 tggaccccac ccacaccaag aatcggagag cctgtcggca atccagccgt cgaccgggtg
 3301 ctgaaaacag tgagcagatg gctggaatcc gcaacaaaga cttggggcgc cccagagagg
 3361 gtcatcattg agcacgtgcg cgaaggcttc gtcactgaga aacgcgctcg agaaatggat
 3421 ggggacatga gaaggcgcgc agcccggaac gccaagctgt ttcaggagat gcaggaaaag
 3481 ctgaatgtgc agggcaaacc cagtcgagcc gatctgtgga gataccagtc agtgcagaga
 3541 cagaactgcc agtgtgccta ttgcgggtcc ccaattacct tttctaatag tgaaatggac
 3601 cacatcgtgc ccagagcagg gcagggatcc accaacacaa gggagaatct ggtcgccgtg
 3661 tgccatcgct gtaaccagtc taagggcaat acacccttcg ctatttgggc aaaaaacact
 3721 tctatcgaag gggtcagtgt gaaggaggcc gtggaacgga ccagacattg ggtcactgat
 3781 accggcatga gaagcactga cttcaagaag ttcaccaagg ctgtggtcga gcggtttcag
 3841 agagcaacaa tggatgagga aatcgacgcc agaagcatgg aatccgtcgc ctggatggct
 3901 aatgagctga ggagccgcgt ggctcagcac ttcgcatccc atggaaccac agtcagggtg
 3961 taccgaggca gcctgacagc agaggctcga cgggcatctg ggatcagtgg aaagctgaaa
 4021 ttctttgatg gcgtggggaa gtccaggctg gatagaaggc accatgctat tgacgctgca
 4081 gtgatcgcat tcacctctga ctatgtggcc gaaacactgg ctgtccgctc aaacctgaaa
 4141 cagagccagg cccaccgaca ggaggctcct cagtggagag agttcaccgg caaggatgca
 4201 gagcatcgag cagcttggag agtgtggtgc cagaagatgg aaaaactgag cgccctgctg
 4261 accgaggacc tgcgagatga ccgggtggtc gtgatgtcta acgtgcgact gcggctggga
 4321 aatggcagtg cccacaagga aaccattggc aaactgtcaa aggtgaaact gtcctctcag
 4381 ctgtcagtca gcgatatcga caaagcaagt tcagaggccc tgtggtgtgc tctgaccaga
 4441 gagcccggat tcgatcctaa ggaaggcctg cccgctaacc ctgagagaca catcagggtg
 4501 aatgggacac atgtctacgc cggggacaat attggactgt ttccagtgtc agcaggaagc
 4561 atcgcactga ggggaggata cgcagagctg ggcagctcct tccaccatgc tcgcgtgtat
 4621 aaaattactt ccggcaagaa acccgcattt gccatgctga gggtgtacac catcgatctg
 4681 ctgccttatc gcaaccagga cctgtttagc gtggaactga agccacagac aatgtccatg
 4741 aggcaggctg agaagaaact gcgcgacgct ctggcaactg ggaatgcaga atatctggga
 4801 tggctggtcg tggatgacga gctggtcgtg gatacatcta agattgccac tgaccaggtc
 4861 aaagcagtgg aggccgaact ggggactatc cgccgatggc gggtggatgg attcttttcc
 4921 ccctctaaac tgagactgag gcctctgcag atgtccaagg aggggatcaa gaaagagtcc
 4981 gctcccgaac tgtctaaaat cattgacaga ccaggatggc tgcccgccgt gaacaagctg
 5041 ttctctgatg gaaatgtcac cgtcgtgcgg agagactctc tgggacgcgt gcgactggag
 5101 agtacagccc acctgcctgt cacttggaag gtgcagtaag aattcggtac cgagctcaag
 5161 cttgcggccg cctatagtgt cacctaaatc gtaacccagc tttcttgtac aaagtggtcg
 5221 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
 5281 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
 5341 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggatgacaa
 5401 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
 5461 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
 5521 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
 5581 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
 5641 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctag
 5701 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtctaga agacaatagc
 5761 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
 5821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
 5881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
 5941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
 6001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
 6061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
 6121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
 6181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
 6241 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
 6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
 6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
 6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
 6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
 6541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
 6601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
 6661 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
 6721 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
 6781 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
 6841 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
 6901 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
 6961 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
 7021 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
 7081 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
 7141 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
 7201 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
 7261 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
 7321 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
 7381 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
 7441 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
 7501 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
 7561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
 7621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
 7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
 7741 gtccaaactc atcaatgtat cttatcatgt ctgttcatga agccccttga gcatctgact
 7801 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
 7861 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
 7921 tagagtttgg caacatatgc ccatatgtaa ctagcataac cccttggggc ctctaaacgg
 7981 gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt ctccttgaag tttccctggt
 8041 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
 8101 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
 8161 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
 8221 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
 8281 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
 8341 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
 8401 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
 8461 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
 8521 cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg
 8581 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
 8641 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
 8701 tgagcttttt ggaattgcga tcgcataact tcgtatagca tacattatac gaagttataa
 8761 gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
 8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
 8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
 8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
 9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
 9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
 9121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
 9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
 9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
 9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
 9361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
 9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
 9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgttacc aatgcttaat
 9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
 9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
 9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
 9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
 9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
 9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
 9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
 9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgtc
10441 cgcgcgtt
//

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