PX165

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: PX165 或 pSpCas9
质粒类型: CRISPR-Cas9载体;基因敲除载体;哺乳动物载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: Cbh
载体大小: 8124bp
5' 测序引物及序列: CTGGAGCACCTGCCTGAAATCACT
3' 测序引物及序列: --
载体标签: 3XFLAG (N ter)
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: Stbl3
宿主细胞(系): 常规哺乳动物细胞
备注: Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)
产品目录号: #48137
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

PX165载体图谱



载体简介

载体序列

LOCUS       pSpCas9 (PX165) Sequencing Result	8214 bp 	DNA	SYN
DEFINITION  pSpCas9 (PX165) Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="pSpCas9 (PX165) Sequencing Result"
                     /mol_type="other DNA"
     rep_origin      complement(268..574)
                     /label="f1_origin"
     terminator      complement(822..1025)
                     /label="bGH_PA_terminator"
     misc_feature    1011..1028
                     /label="BGH_rev_primer"
     CDS             complement(1053..5324)
                     /label="ORF frame 2"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKY
                     SIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATR
                     LKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGN
                     IVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNS
                     DVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGL
                     FGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKN
                     LSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFD
                     QSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIP
                     HQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKS
                     EETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKV
                     KYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVED
                     RFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLF
                     DDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDS
                     LTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENI
                     VIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQ
                     NGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEV
                     VKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQ
                     ILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNA
                     VVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLG
                     ITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKG
                     NELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVI
                     LADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTST
                     KEVLDATLIHQSITGLYETRIDLSQLGGDKRPAATKKAGQAKKKK*"
     CDS             2688..5345
                     /label="ORF frame 3"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5226..5249)
                     /label="SV40 NLS"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5229..5249)
                     /label="SV40 NLS"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5229..5243)
                     /label="NLS"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5256..5279)
                     /label="FLAG"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5280..5300)
                     /label="FLAG"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5301..5321)
                     /label="FLAG"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     misc_feature    complement(5860..6129)
                     /label="CAG_enhancer"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     rep_origin      complement(6329..6948)
                     /label="pBR322_origin"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     gene            complement(7103..7963)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVHIVVGQPVDVQFLVHVHIPPILQVVQVQLLVLQLGVFHGVFF
                     QDLAAQLFDALFDPLHSLAAVLLSLLGGLVLSGHFDHDVLGLVPAHHFHELVHHLHCL
                     QDALLNGGAAGQIGNVLVQAIALAGHLGFLDVLFKGQAVVVDQLHEVSVGEAVGLQEI
                     QDCLAGLLVPDAVDQLPAQPAPAGVSPPLQLLHHFVVEQVGIGFQPFLDHLSVLKQCQ
                     GQHDIFQNVLVFLIVQEVLVLDNFQQIVVCAQGGVEPIFHAGDFHGVEALDFLEVVLF
                     QLLHGHFPVGLEQQVHDGLFLLAAQEGGLSHSLGHVFHFGQLVIHGEVLVQQAVLGQH
                     LLVGQVLIEVGHPLDEALGGSALVHHFLEVPGGDGFLALSGHPGESAVSPGQRAHVVG
                     DAEGQDLLDLFPVVLQEWVKIFLPPQNGVQLSQVDLVGDAAVVEGPLLPQQVLSVQLH
                     EQFLGAVHLFQDGLDELVELFLAGSAVNVAGVAVLALVEENLFVLLRQLLPHESFQQG
                     QVLVVLVVSLDHRGAQGGLGDLGVHSQDVAQQDGVGQVLGGQKQVGVLVADLGQQVVQ
                     VVVVGVLAQLQFGILGQVEVALEVGGQAQAQGNQVSEQAILLLAGQLGDQIFQPSALA
                     QSGRQDGLGVHAAGVDGVFLEQLVVGLHQLDEQLVHVAVVGVQVALDQEVAPELDHVG
                     QGQIDQPQVGLVGAVHQFLSQVVDGGVLLVVGHLVHDVAEDGVPLVLLILFHQEGLFQ
                     SVEEAVVHLGHLVAEDLLQIADPVLPSGVSSSGGSLQPGGLGCFAAVEQQGSDQVLLD
                     AVPVGVAQHLEFLAGHLVLVGDHGPAHRVGADVQADAVLLVGCWDSVDTDLPLLLWGH
                     LIVIVFVINIMILVVSVVVLIVHGGTGPT*"
     CDS             complement(7103..7963)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(8005..8033)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(8192..8214)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT
   61 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC
  121 TGAGAGTGCA CCATAAAATT GTAAACGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT
  181 GTTAAATCAG CTCATTTTTT AACCAATAGA CCGAAATCGG CAAAATCCCT TATAAATCAA
  241 AAGAATAGCC CGAGATAGAG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA
  301 AGAACGTGGA CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC
  361 GTGAACCATC ACCCAAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA
  421 ACCCTAAAGG GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA
  481 AGGAAGGGAA GAAAGCGAAA GGAGCGGGCG CTAAGGCGCT GGCAAGTGTA GCGGTCACGC
  541 TGCGCGTAAC CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TACTATGGTT
  601 GCTTTGACGT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG
  661 GCGCCCCTGC AGGCAGCTGC GCGCTCGCTC GCTCACTGAG GCCGCCCGGG CAAAGCCCGG
  721 GCGTCGGGCG ACCTTTGGTC GCCCGGCCTC AGTGAGCGAG CGAGCGCGCA GAGAGGGAGT
  781 GGCCAACTCC ATCACTAGGG GTTCCTGCGG CCGCTCCCCA GCATGCCTGC TATTCTCTTC
  841 CCAATCCTCC CCCTTGCTGT CCTGCCCCAC CCCACCCCCC AGAATAGAAT GACACCTACT
  901 CAGACAATGC GATGCAATTT CCTCATTTTA TTAGGAAAGG ACAGTGGGAG TGGCACCTTC
  961 CAGGGTCAAG GAAGGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC
 1021 AGTCGAGGCT GATCAGCGAG CTCTAGGAAT TCTTACTTTT TCTTTTTTGC CTGGCCGGCC
 1081 TTTTTCGTGG CCGCCGGCCT TTTGTCGCCT CCCAGCTGAG ACAGGTCGAT CCGTGTCTCG
 1141 TACAGGCCGG TGATGCTCTG GTGGATCAGG GTGGCGTCCA GCACCTCTTT GGTGCTGGTG
 1201 TACCTCTTCC GGTCGATGGT GGTGTCAAAG TACTTGAAGG CGGCAGGGGC TCCCAGATTG
 1261 GTCAGGGTAA ACAGGTGGAT GATATTCTCG GCCTGCTCTC TGATGGGCTT ATCCCGGTGC
 1321 TTGTTGTAGG CGGACAGCAC TTTGTCCAGA TTAGCGTCGG CCAGGATCAC TCTCTTGGAG
 1381 AACTCGCTGA TCTGCTCGAT GATCTCGTCC AGGTAGTGCT TGTGCTGTTC CACAAACAGC
 1441 TGTTTCTGCT CATTATCCTC GGGGGAGCCC TTCAGCTTCT CATAGTGGCT GGCCAGGTAC
 1501 AGGAAGTTCA CATATTTGGA GGGCAGGGCC AGTTCGTTTC CCTTCTGCAG TTCGCCGGCA
 1561 GAGGCCAGCA TTCTCTTCCG GCCGTTTTCC AGCTCGAACA GGGAGTACTT AGGCAGCTTG
 1621 ATGATCAGGT CCTTTTTCAC TTCTTTGTAG CCCTTGGCTT CCAGAAAGTC GATGGGATTC
 1681 TTCTCGAAGC TGCTTCTTTC CATGATGGTG ATCCCCAGCA GCTCTTTCAC ACTCTTCAGT
 1741 TTCTTGGACT TGCCCTTTTC CACTTTGGCC ACCACCAGCA CAGAATAGGC CACGGTGGGG
 1801 CTGTCGAAGC CGCCGTACTT CTTAGGGTCC CAGTCCTTCT TTCTGGCGAT CAGCTTATCG
 1861 CTGTTCCTCT TGGGCAGGAT AGACTCTTTG CTGAAGCCGC CTGTCTGCAC CTCGGTCTTT
 1921 TTCACGATAT TCACTTGGGG CATGCTCAGC ACTTTCCGCA CGGTGGCAAA ATCCCGGCCC
 1981 TTATCCCACA CGATCTCCCC GGTTTCGCCG TTTGTCTCGA TCAGAGGCCG CTTCCGGATC
 2041 TCGCCGTTGG CCAGGGTAAT CTCGGTCTTG AAAAAGTTCA TGATGTTGCT GTAGAAGAAG
 2101 TACTTGGCGG TAGCCTTGCC GATTTCCTGC TCGCTCTTGG CGATCATCTT CCGCACGTCG
 2161 TACACCTTGT AGTCGCCGTA CACGAACTCG CTTTCCAGCT TAGGGTACTT TTTGATCAGG
 2221 GCGGTTCCCA CGACGGCGTT CAGGTAGGCG TCGTGGGCGT GGTGGTAGTT GTTGATCTCG
 2281 CGCACTTTGT AAAACTGGAA ATCCTTCCGG AAATCGGACA CCAGCTTGGA CTTCAGGGTG
 2341 ATCACTTTCA CTTCCCGGAT CAGCTTGTCA TTCTCGTCGT ACTTAGTGTT CATCCGGGAG
 2401 TCCAGGATCT GTGCCACGTG CTTTGTGATC TGCCGGGTTT CCACCAGCTG TCTCTTGATG
 2461 AAGCCGGCCT TATCCAGTTC GCTCAGGCCG CCTCTCTCGG CCTTGGTCAG ATTGTCGAAC
 2521 TTTCTCTGGG TAATCAGCTT GGCGTTCAGC AGCTGCCGCC AGTAGTTCTT CATCTTCTTC
 2581 ACGACCTCTT CGGAGGGCAC GTTGTCGCTC TTGCCCCGGT TCTTGTCGCT TCTGGTCAGC
 2641 ACCTTGTTGT CGATGGAGTC GTCCTTCAGA AAGCTCTGAG GCACGATATG GTCCACATCG
 2701 TAGTCGGACA GCCGGTTGAT GTCCAGTTCC TGGTCCACGT ACATATCCCG CCCATTCTGC
 2761 AGGTAGTACA GGTACAGCTT CTCGTTCTGC AGCTGGGTGT TTTCCACGGG GTGTTCTTTC
 2821 AGGATCTGGC TGCCCAGCTC TTTGATGCCC TCTTCGATCC GCTTCATTCT CTCGCGGCTG
 2881 TTCTTCTGTC CCTTCTGGGT GGTCTGGTTC TCTCTGGCCA TTTCGATCAC GATGTTCTCG
 2941 GGCTTGTGCC GGCCCATCAC TTTCACGAGC TCGTCCACCA CCTTCACTGT CTGCAGGATG
 3001 CCCTTCTTAA TGGCGGGGCT GCCGGCCAGA TTGGCAATGT GCTCGTGCAG GCTATCGCCC
 3061 TGGCCGGACA CCTGGGCTTT CTGGATGTCC TCTTTAAAGG TCAGGCTGTC GTCGTGGATC
 3121 AGCTGCATGA AGTTTCTGTT GGCGAAGCCG TCGGACTTCA GGAAATCCAG GATTGTCTTG
 3181 CCGGACTGCT TGTCCCGGAT GCCGTTGATC AGCTTCCGGC TCAGCCTGCC CCAGCCGGTG
 3241 TATCTCCGCC GCTTCAGCTG CTTCATCACT TTGTCGTCGA ACAGGTGGGC ATAGGTTTTC
 3301 AGCCGTTCCT CGATCATCTC TCTGTCCTCA AACAGTGTCA GGGTCAGCAC GATATCTTCC
 3361 AGAATGTCCT CGTTTTCCTC ATTGTCCAGG AAGTCCTTGT CCTTGATAAT TTTCAGCAGA
 3421 TCGTGGTATG TGCCCAGGGA GGCGTTGAAC CGATCTTCCA CGCCGGAGAT TTCCACGGAG
 3481 TCGAAGCACT CGATTTTCTT GAAGTAGTCC TCTTTCAGCT GCTTCACGGT CACTTTCCGG
 3541 TTGGTCTTGA ACAGCAGGTC CACGATGGCC TTTTTCTGCT CGCCGCTCAG GAAGGCGGGC
 3601 TTTCTCATTC CCTCGGTCAC GTATTTCACT TTGGTCAGCT CGTTATACAC GGTGAAGTAC
 3661 TCGTACAGCA GGCTGTGCTT GGGCAGCACC TTCTCGTTGG GCAGGTTCTT ATCGAAGTTG
 3721 GTCATCCGCT CGATGAAGCT CTGGGCGGAA GCGCCCTTGT CCACCACTTC CTCGAAGTTC
 3781 CAGGGGGTGA TGGTTTCCTC GCTCTTTCTG GTCATCCAGG CGAATCTGCT GTTTCCCCTG
 3841 GCCAGAGGGC CCACGTAGTA GGGGATGCGG AAGGTCAGGA TCTTCTCGAT CTTTTCCCGG
 3901 TTGTCCTTCA GGAATGGGTA AAAATCTTCC TGCCGCCGCA GAATGGCGTG CAGCTCTCCC
 3961 AGGTGGATCT GGTGGGGGAT GCTGCCGTTG TCGAAGGTCC GCTGCTTCCG CAGCAGGTCC
 4021 TCTCTGTTCA GCTTCACGAG CAGTTCCTCG GTGCCGTCCA TCTTTTCCAG GATGGGCTTG
 4081 ATGAACTTGT AGAACTCTTC CTGGCTGGCT CCGCCGTCAA TGTAGCCGGC GTAGCCGTTC
 4141 TTGCTCTGGT CGAAGAAAAT CTCTTTGTAC TTCTCAGGCA GCTGCTGCCG CACGAGAGCT
 4201 TTCAGCAGGG TCAGGTCCTG GTGGTGCTCG TCGTATCTCT TGATCATAGA GGCGCTCAGG
 4261 GGGGCCTTGG TGATCTCGGT GTTCACTCTC AGGATGTCGC TCAGCAGGAT GGCGTCGGAC
 4321 AGGTTCTTGG CGGCCAGAAA CAGGTCGGCG TACTGGTCGC CGATCTGGGC CAGCAGGTTG
 4381 TCCAGGTCGT CGTCGTAGGT GTCCTTGCTC AGCTGCAGTT TGGCATCCTC GGCCAGGTCG
 4441 AAGTTGCTCT TGAAGTTGGG GGTCAGGCCC AGGCTCAGGG CAATCAGGTT TCCGAACAGG
 4501 CCATTCTTCT TCTCGCCGGG CAGCTGGGCG ATCAGATTTT CCAGCCGTCT GCTCTTGCTC
 4561 AGTCTGGCAG ACAGGATGGC CTTGGCGTCC ACGCCGCTGG CGTTGATGGG GTTTTCCTCG
 4621 AACAGCTGGT TGTAGGTCTG CACCAGCTGG ATGAACAGCT TGTCCACGTC GCTGTTGTCG
 4681 GGGTTCAGGT CGCCCTCGAT CAGGAAGTGG CCCCGGAACT TGATCATGTG GGCCAGGGCC
 4741 AGATAGATCA GCCGCAGGTC GGCCTTGTCG GTGCTGTCCA CCAGTTTCTT TCTCAGGTGG
 4801 TAGATGGTGG GGTACTTCTC GTGGTAGGCC ACCTCGTCCA CGATGTTGCC GAAGATGGGG
 4861 TGCCGCTCGT GCTTCTTATC CTCTTCCACC AGGAAGGACT CTTCCAGTCT GTGGAAGAAG
 4921 CTGTCGTCCA CCTTGGCCAT CTCGTTGCTG AAGATCTCTT GCAGATAGCA GATCCGGTTC
 4981 TTCCGTCTGG TGTATCTTCT TCTGGCGGTT CTCTTCAGCC GGGTGGCCTC GGCTGTTTCG
 5041 CCGCTGTCGA ACAGCAGGGC TCCGATCAGG TTCTTCTTGA TGCTGTGCCG GTCGGTGTTG
 5101 CCCAGCACCT TGAATTTCTT GCTGGGCACC TTGTACTCGT CGGTGATCAC GGCCCAGCCC
 5161 ACAGAGTTGG TGCCGATGTC CAGGCCGATG CTGTACTTCT TGTCGGCTGC TGGGACTCCG
 5221 TGGATACCGA CCTTCCGCTT CTTCTTTGGG GCCATCTTAT CGTCATCGTC TTTGTAATCA
 5281 ATATCATGAT CCTTGTAGTC TCCGTCGTGG TCCTTATAGT CCATGGTGGC ACCGGTCCAA
 5341 CCTGAAAAAA AGTGATTTCA GGCAGGTGCT CCAGGTAATT AAACATTAAT ACCCCACCAA
 5401 CCAACCATCC CTTAAACCCT TACCTCTTGC TCAGCTAATT ACAGCCCGGA GGAGAAGGGC
 5461 CGTCCCGCCC GCTCACCTGT GGGAGTAACG CGGTCAGTCA GAGCCGGGGC GGGCGGCGCG
 5521 AGGCGGCGGC GGAGCGGGGC ACGGGGCGAA GGCAGCGCGC AGCGACTCCC GCCCGCCGCG
 5581 CGCTTCGCTT TTTATAGGGC CGCCGCCGCC GCCGCCTCGC CATAAAAGGA AACTTTCGGA
 5641 GCGCGCCGCT CTGATTGGCT GCCGCCGCAC CTCTCCGCCT CGCCCCGCCC CGCCCCTCGC
 5701 CCCGCCCCGC CCCGCCTGGC GCGCGCCCCC CCCCCCCCCC CGCCCCCATC GCTGCACAAA
 5761 ATAATTAAAA AATAAATAAA TACAAAATTG GGGGTGGGGA GGGGGGGGAG ATGGGGAGAG
 5821 TGAAGCAGAA CGTGGGGCTC ACCTCGACCA TGGTAATAGC GATGACTAAT ACGTAGATGT
 5881 ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ACAATGCCAG GCGGGCCATT
 5941 TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA
 6001 AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG
 6061 TTACTATTGA CGTCAATGGG CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG
 6121 TAAGTTATGT AACGGGTACC TCTAGAGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC
 6181 TCCCTCTCTG CGCGCTCGCT CGCTCACTGA GGCCGGGCGA CCAAAGGTCG CCCGACGCCC
 6241 GGGCGGCCTC AGTGAGCGAG CGAGCGCGCA GCTGCCTGCA GGACATGTGA GCAAAAGGCC
 6301 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
 6361 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
 6421 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
 6481 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA
 6541 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
 6601 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
 6661 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
 6721 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
 6781 GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
 6841 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
 6901 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
 6961 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
 7021 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
 7081 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
 7141 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
 7201 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGGCTTCCA CGCTCACCGG
 7261 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
 7321 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
 7381 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
 7441 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
 7501 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
 7561 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
 7621 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
 7681 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
 7741 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
 7801 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
 7861 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
 7921 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
 7981 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
 8041 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT
 8101 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC
 8161 GTctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccgg

//

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