MEM培养基Gibco 11095080

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规格: 500ml 500ml*10

联系方式:I47-825O-882O

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Gibco MEM培养基产品基本信息

产品名称: Gibco MEM培养基、Gibco MEM培养基
产品又名: Gibco MEM细胞培养基、Gibco MEM细胞培养基
英文名称: Gibco MEM
培养基类型: 哺乳细胞培养基
级别: For Mammalian Cell Culture
品牌: Gibco
产品目录号: 11095080、11095-080
谷氨酰胺: 含L-谷氨酰胺
酚红: 含酚红
丙酮酸钠: 不含丙酮酸钠
HEPES 不含HEPES
环保特征: 可循环使用包装
产品规格: 500ml
血清添加: 使用时,正常浓度添加血清。
运输条件: 室温
产品外观: 红色液体
颜色与澄清度: 红色透明至微不透明液体。
保存条件: 密封,2-8°C保存。
注意事项: 避免呼入和皮肤接触。
Gibco: 新鲜酵母浸出液
热灭活马血清

Gibco MEM培养基图片

MEM

Gibco MEM细胞培养基产品描述:

MEM(Minimum Essential Medium)是最常用的细胞培养基之一。MEM可用于多种悬浮和粘附的哺乳动物细胞,包括HeLa、BHK-21、293、HEP-2、HT-1080、MCF-7、成纤维细胞和原代大鼠星形胶质细胞。我们为一系列细胞培养应用提供多种MEM修饰。使用介质选择器工具找到正确的公式。

Gibco MEM培养基使用方法

MEM是由harryeagle开发的,基于他早期的basicmedium Eagle(BME)公式。许多其他的MEM修饰,包括格拉斯哥的MEM,MEMα,DMEM和Temin的修饰。MEM可与Earle's盐类一起用于二氧化碳培养箱,或与Hanks's盐类一起用于无二氧化碳培养箱。这种产品是用厄尔盐制成的。MEM不含蛋白质、脂类或生长因子。因此,MEM需要补充,通常添加10%的胎牛血清(FBS)。MEM使用碳酸氢钠缓冲系统(2.2 g/L),因此需要5–10%的CO2环境来维持生理pH值。

Gibco MEM培养基质量控制了体系

MEM在纽约格兰德岛符合cGMP标准的工厂生产。该设施在FDA注册为医疗器械制造商,并通过ISO 13485标准认证。为了保持供应链的连续性,我们提供了在苏格兰工厂(31095-029)生产的相同MEM产品。该工厂在FDA注册为医疗器械制造商,并通过ISO 13485标准认证。

Gibco MEM培养基配方

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211 126 0.5971564
L-Cystine 2HCl 313 31 0.09904154
L-Glutamine 146 292 2
L-Histidine hydrochloride-H2O 210 42 0.2
L-Isoleucine 131 52 0.39694658
L-Leucine 131 52 0.39694658
L-Lysine hydrochloride 183 73 0.3989071
L-Methionine 149 15 0.10067114
L-Phenylalanine 165 32 0.19393939
L-Threonine 119 48 0.40336135
L-Tryptophan 204 10 0.04901961
L-Tyrosine disodium salt dihydrate 261 52 0.19923371
L-Valine 117 46 0.3931624
Vitamins
Choline chloride 140 1 0.007142857
D-Calcium pantothenate 477 1 0.002096436
Folic Acid 441 1 0.002267574
Niacinamide 122 1 0.008196721
Pyridoxal hydrochloride 204 1 0.004901961
Riboflavin 376 0.1 2.66E-04
Thiamine hydrochloride 337 1 0.002967359
i-Inositol 180 2 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 200 1.8018018
Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.8139166
Potassium Chloride (KCl) 75 400 5.3333335
Sodium Bicarbonate (NaHCO3) 84 2200 26.190475
Sodium Chloride (NaCl) 58 6800 117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 140 1.0144928
Other Components
D-Glucose (Dextrose) 180 1000 5.5555553
Phenol Red 376.4 10 0.026567481


Gibco细胞培养基选择指南

Transfection Reagent (细胞转染试剂)
产品描述 规格 货号
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培养基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培养基)
产品描述 规格 货号
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培养基)
产品描述 规格 货号
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培养基)
产品描述 规格 货号
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基础培养基
产品描述 规格 货号
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 细胞减血清培养基
产品描述 规格 货号
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 细胞培养缓冲液
产品描述 规格 货号
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆虫培养基
产品描述 规格 货号
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 细胞培养试剂
产品描述 规格 货号
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青链霉素双抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(无菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

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Gibco MEM培养基产品应用举例

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