| 保藏机构: | NCTC |
|---|---|
| 菌种名称: | NCTC9336 , NCTC 9336 |
| 英文名称: | Prevotella melaninogenica |
| 中文名称 | 产黑素普雷沃菌、杀虫贪铜菌 |
| 存储人: | (Oliver and Wherry) Shah and Collins |
| 来源历史: | << NCTC << Sharpe << L. P. Garrod, St. Bartholomew's Hospital, London |
| 其他保藏中心编号: | NCTC 9336 |
| 分离来源: | 文森特牙龈炎 |
| 菌种特性 | |
| 备注： | |
| 培养条件： | 37°C |
| 生物安全等级: | 2 |
| 氧气需求: | 需氧 |
| 培养基: |
CHOPPED MEAT MEDIUM
Ground beef (fat free) 500.0 g
1N NaOH 25.0 mL
Distilled water 1.0 L
Peptone 30.0 g
Yeast extract 5.0 g
L-cysteine·HCl 0.5 g
K2HPO4 5.0 g
0.025% Resazurin solution 4.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.
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| 模式菌株: | |
| 参考文献: |
NCTC9336
价格：3200元
代购价格：询价
联系方式：I47-825O-882O
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