CIP106682

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CIP106682标准菌株基本信息

保藏机构: CIP
菌种名称: CIP106682, CIP 106682
英文名称: Rhodopseudomonas rhenobacensis
中文名称 大红假单胞菌
存储人: Hougardy et al.
来源历史:
<< DSMZ << J.-H. Klemme, R1
其他保藏中心编号: DSM 12706; CIP 106682
分离来源: 富营养化池塘底泥
菌种特性  
备注:  
培养条件: 28°C
生物安全等级: 1
氧气需求: 需氧
培养基: TSB培养基:66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone 15.0 g
Soytone 5.0 g
NaCl 5.0 g
Agar 15.0 g
Distilled water 1.0 L
Adjust pH to 7.3.
996
RHODOSPIRILLACEAE MEDIUM (reduce yeast extract and pH)
(NH4)-acetate 0.50 g
CaCl2 · 2 H2O 0.05 g
Distilled water 1000 mL
Fe(III) citrate solution (0.1% in H2O) 5.00 mL
KH2PO4 0.50 g
L-Cysteiniumchloride 0.30 g
MgSO4 · 7 H2O 0.40 g
Na2-succinate 1.00 g
NaCl 0.40 g
NH4Cl 0.40 g
Resazurin (0.1%) 0.50 mL
Trace element solution SL-6 (see below) 1.00 mL
Vitamin B12 solution (10 mg in 100 mL H2O) 0.40 mL
Yeast extract 0.20 g
Reduce Medium 903 the yeast extract concentration to 0.2 g/L. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 mL in 15 mL tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121°C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Adjust pH to 5.7.
Trace element solution SL-6
CoCl2· 6 H2O 0.20 g
CuCl2 ·2 H2O 0.01 g
Distilled water 1000 mL
H3BO3 0.30 g
MnCl2·4 H2O 0.03 g
Na2MoO4·2 H2O 0.03 g
NiCl2· 6 H2O 0.02 g
ZnSO4·7 H2O 0.10 g
模式菌株:
参考文献:
B4513 
Hougardy, A., B. J. Tindall, and J. H. Klemme. 2000. Rhodopseudomonas rhenobacensis sp. nov., a new nitrate-reducing purple non-sulfur bacterium. Int.J.Syst.Evol.Microbiol. 50 : 985-992. 

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