| 质粒名称: | pX330a dCas9-KRAB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92361 |
| 存储实验室: | Tatjana Sauka-Spengler |
|
载体骨架基本信息 |
|
| 载体名称: | x330 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4332 bp |
| 完整质粒大小: | 8731 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | dCas9-KRAB |
| 物种来源: | -- |
| 插入大小: | 4399 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CAG |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | tctgactgaccgcgttactc |
| 3' 测序引物 | aggaaaggacagtgggagtg |
|
文献引用方法 |
|
| 材料和方法部分: | pX330a dCas9-KRAB was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 92361 ; http://n2t.net/addgene:92361 ; RRID:Addgene_92361) |
| 参考文献部分: | Genome and epigenome engineering CRISPR toolkit for in vivo modulation of cis-regulatory interactions and gene expression in the chicken embryo. Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T. Development. 2018 Feb 23;145(4). pii: dev.160333. doi: 10.1242/dev.160333. 10.1242/dev.160333 PubMed 29386245 |
买家导航
pX330a dCas9-KRAB载体质粒基本信息
pX330a dCas9-KRAB质粒图谱载体图谱和pX330a dCas9-KRAB载体序列质粒序列多克隆位点信息
pX330a dCas9-KRAB质粒载体简介
pX330a dCas9-KRAB质粒序列载体序列
LOCUS pX330a dCas9-KRAB 8731 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Modified CAG promoter-containing vector for ubiquitous expression of
catalytically inactive Cas9 fused to KRAB repressor domain. For
targeted enhancer inactivation in chicken embryos..
ACCESSION .
VERSION .
KEYWORDS pX330a dCas9-KRAB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8731)
AUTHORS Williams RM, Senanayake U, Artibani M, Taylor G, Wells D, Ahmed AA,
Sauka-Spengler T
TITLE Genome and epigenome engineering CRISPR toolkit for probing in vivo
cis-regulatory interactions in the chicken embryo
JOURNAL BioRxiv
REFERENCE 2 (bases 1 to 8731)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8731
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 24..612
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 513..532
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 674..914
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 674..694
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 845..864
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 941..1016
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
enhancer 1113..1398
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter 1400..1677
/label=chicken beta-actin promoter
intron 1678..1906
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
CDS 1927..1947
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1957..6060
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=Cas9m4
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the mutations D10A, D839A,
H840A, and N863A (Mali et al., 2013)"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6073..6093
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6127..6321
/codon_start=1
/gene="ZNF10"
/gene_synonym="KOX1"
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
CDS 6388..6408
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(6445..6462)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6451..6658
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 6667..6807
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 6667..6796
/label=AAV2 ITR
rep_origin 6882..7337
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6969..6988)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7179..7200
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7354..7373)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7473..7495
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7533..7551)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7619..7723
/gene="bla"
/label=AmpR promoter
CDS 7724..8584
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7942..7961)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
61 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
121 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
181 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
241 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
301 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
361 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
421 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
481 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
541 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
601 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
661 tttgctcaca tgtgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
721 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
781 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
841 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
901 cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc tagaaatagc
961 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
1021 ttgttttaga gctagaaata gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc
1081 caattctgca gacaaatggc tctagaggta cccgttacat aacttacggt aaatggcccg
1141 cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact
1201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
1261 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
1321 cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
1381 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
1441 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
1501 ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
1561 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
1621 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
1681 gtcgctgcga cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
1741 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
1801 gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt
1861 aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg gaccggtgcc
1921 accatgccca agaagaagag gaaggtggga agggggatgg acaagaagta ctccattggg
1981 ctcgctatcg gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg
2041 agcaaaaaat tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt
2101 ggcgccctcc tgttcgactc cggggagacg gccgaagcca cgcggctcaa aagaacagca
2161 cggcgcagat atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat
2221 gagatggcta aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag
2281 gaggataaaa agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac
2341 catgaaaagt acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag
2401 gctgacttgc ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc
2461 ctcatcgagg gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg
2521 gttcagactt acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc
2581 aaagcaatcc tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag
2641 ctccctgggg agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg
2701 acccccaact ttaaatctaa cttcgacctg gccgaagatg ccaagcttca actgagcaaa
2761 gacacctacg atgatgatct cgacaatctg ctggcccaga tcggcgacca gtacgcagac
2821 ctttttttgg cggcaaagaa cctgtcagac gccattctgc tgagtgatat tctgcgagtg
2881 aacacggaga tcaccaaagc tccgctgagc gctagtatga tcaagcgcta tgatgagcac
2941 caccaagact tgactttgct gaaggccctt gtcagacagc aactgcctga gaagtacaag
3001 gaaattttct tcgatcagtc taaaaatggc tacgccggat acattgacgg cggagcaagc
3061 caggaggaat tttacaaatt tattaagccc atcttggaaa aaatggacgg caccgaggag
3121 ctgctggtaa agcttaacag agaagatctg ttgcgcaaac agcgcacttt cgacaatgga
3181 agcatccccc accagattca cctgggcgaa ctgcacgcta tcctcaggcg gcaagaggat
3241 ttctacccct ttttgaaaga taacagggaa aagattgaga aaatcctcac atttcggata
3301 ccctactatg taggccccct cgcccgggga aattccagat tcgcgtggat gactcgcaaa
3361 tcagaagaga ccatcactcc ctggaacttc gaggaagtcg tggataaggg ggcctctgcc
3421 cagtccttca tcgaaaggat gactaacttt gataaaaatc tgcctaacga aaaggtgctt
3481 cctaaacact ctctgctgta cgagtacttc acagtttata acgagctcac caaggtcaaa
3541 tacgtcacag aagggatgag aaagccagca ttcctgtctg gagagcagaa gaaagctatc
3601 gtggacctcc tcttcaagac gaaccggaaa gttaccgtga aacagctcaa agaggactat
3661 ttcaaaaaga ttgaatgttt cgactctgtt gaaatcagcg gagtggagga tcgcttcaac
3721 gcatccctgg gaacgtatca cgatctcctg aaaatcatta aagacaagga cttcctggac
3781 aatgaggaga acgaggacat tcttgaggac attgtcctca cccttacgtt gtttgaagat
3841 agggagatga ttgaagaacg cttgaaaact tacgctcatc tcttcgacga caaagtcatg
3901 aaacagctca agaggcgccg atatacagga tgggggcggc tgtcaagaaa actgatcaat
3961 gggatccgag acaagcagag tggaaagaca atcctggatt ttcttaagtc cgatggattt
4021 gccaaccgga acttcatgca gttgatccat gatgactctc tcacctttaa ggaggacatc
4081 cagaaagcac aagtttctgg ccagggggac agtctccacg agcacatcgc taatcttgca
4141 ggtagcccag ctatcaaaaa gggaatactg cagaccgtta aggtcgtgga tgaactcgtc
4201 aaagtaatgg gaaggcataa gcccgagaat atcgttatcg agatggcccg agagaaccaa
4261 actacccaga agggacagaa gaacagtagg gaaaggatga agaggattga agagggtata
4321 aaagaactgg ggtcccaaat ccttaaggaa cacccagttg aaaacaccca gcttcagaat
4381 gagaagctct acctgtacta cctgcagaac ggcagggaca tgtacgtgga tcaggaactg
4441 gacatcaatc ggctctccga ctacgacgtg gctgctatcg tgccccagtc ttttctcaaa
4501 gatgattcta ttgataataa agtgttgaca agatccgata aagctagagg gaagagtgat
4561 aacgtcccct cagaagaagt tgtcaagaaa atgaaaaatt attggcggca gctgctgaac
4621 gccaaactga tcacacaacg gaagttcgat aatctgacta aggctgaacg aggtggcctg
4681 tctgagttgg ataaagccgg cttcatcaaa aggcagcttg ttgagacacg ccagatcacc
4741 aagcacgtgg cccaaattct cgattcacgc atgaacacca agtacgatga aaatgacaaa
4801 ctgattcgag aggtgaaagt tattactctg aagtctaagc tggtctcaga tttcagaaag
4861 gactttcagt tttataaggt gagagagatc aacaattacc accatgcgca tgatgcctac
4921 ctgaatgcag tggtaggcac tgcacttatc aaaaaatatc ccaagcttga atctgaattt
4981 gtttacggag actataaagt gtacgatgtt aggaaaatga tcgcaaagtc tgagcaggaa
5041 ataggcaagg ccaccgctaa gtacttcttt tacagcaata ttatgaattt tttcaagacc
5101 gagattacac tggccaatgg agagattcgg aagcgaccac ttatcgaaac aaacggagaa
5161 acaggagaaa tcgtgtggga caagggtagg gatttcgcga cagtccggaa ggtcctgtcc
5221 atgccgcagg tgaacatcgt taaaaagacc gaagtacaga ccggaggctt ctccaaggaa
5281 agtatcctcc cgaaaaggaa cagcgacaag ctgatcgcac gcaaaaaaga ttgggacccc
5341 aagaaatacg gcggattcga ttctcctaca gtcgcttaca gtgtactggt tgtggccaaa
5401 gtggagaaag ggaagtctaa aaaactcaaa agcgtcaagg aactgctggg catcacaatc
5461 atggagcgat caagcttcga aaaaaacccc atcgactttc tcgaggcgaa aggatataaa
5521 gaggtcaaaa aagacctcat cattaagctt cccaagtact ctctctttga gcttgaaaac
5581 ggccggaaac gaatgctcgc tagtgcgggc gagctgcaga aaggtaacga gctggcactg
5641 ccctctaaat acgttaattt cttgtatctg gccagccact atgaaaagct caaagggtct
5701 cccgaagata atgagcagaa gcagctgttc gtggaacaac acaaacacta ccttgatgag
5761 atcatcgagc aaataagcga attctccaaa agagtgatcc tcgccgacgc taacctcgat
5821 aaggtgcttt ctgcttacaa taagcacagg gataagccca tcagggagca ggcagaaaac
5881 attatccact tgtttactct gaccaacttg ggcgcgcctg cagccttcaa gtacttcgac
5941 accaccatag acagaaagcg gtacacctct acaaaggagg tcctggacgc cacactgatt
6001 catcagtcaa ttacggggct ctatgaaaca agaatcgacc tctctcagct cggtggagac
6061 agcagggctg accccaagaa gaagaggaag gtggctagcg atgctaagtc actgactgcc
6121 tggtcccgga cactggtgac cttcaaggat gtgtttgtgg acttcaccag ggaggagtgg
6181 aagctgctgg acactgctca gcagatcctg tacagaaatg tgatgctgga gaactataag
6241 aacctggttt ccttgggtta tcagcttact aagccagatg tgatcctccg gttggagaag
6301 ggagaagagc cctggctggt ggagagagaa attcaccaag agacccatcc tgattcagag
6361 actgcatttg aaatcaaatc atcagttccg aaaaagaaac gcaaagttgc tagctgataa
6421 gaattcctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
6481 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
6541 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
6601 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagc
6661 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
6721 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
6781 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
6841 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
6901 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
6961 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
7021 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
7081 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
7141 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
7201 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
7261 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
7321 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
7381 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
7441 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
7501 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
7561 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
7621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
7681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
7741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
7801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
7861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
7921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
7981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
8041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
8101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
8161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
8221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
8281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
8341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
8401 ctggtttatt gctgataaat ctggagccgg tgagcgtgga agccgcggta tcattgcagc
8461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
8521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
8581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
8641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
8701 tgagttttcg ttccactgag cgtcagaccc c
//
