| 质粒名称: | pVenus-N1 (804) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61854 |
| 存储实验室: | Jonathon Pines |
|
载体骨架基本信息 |
|
| 载体名称: | pEYFP-N1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 4733bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 无 |
| 标签/融合蛋白: | Venus (C terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: |
pVenus-N1 (804) was a gift from Jonathon Pines (Addgene plasmid # 61854 ; http://n2t.net/addgene:61854 ; RRID:Addgene_61854)
|
pVenus-N1 (804)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pVenus-N1 (804)载体质粒基本信息
pVenus-N1 (804)质粒图谱载体图谱和pVenus-N1 (804)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pVenus-N1 (804)质粒载体简介
pVenus-N1 (804)质粒序列载体序列
LOCUS pVenus-N1 (804) 4733 bp DNA circular SYN 25-AUG-2024
DEFINITION empty backbone for tagging proteins at the C-terminus with Venus.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4733)
TITLE Pines Lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4733)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..4733
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 129..432
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 433..636
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 587..607
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 659..739
/label=MCS
/note="multiple cloning site"
regulatory 741..750
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 747..1466
/codon_start=1
/product="yellow fluorescent protein (YFP) with fast and
efficient maturation (Nagai et al., 2002)"
/label=Venus
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(792..813)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1053..1072)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1400..1421
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 1589..1710
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1626..1645)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1680..1699
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1717..2172)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1854..1875)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2066..2085
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2199..2303
/gene="bla"
/label=AmpR promoter
promoter 2305..2662
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2305..2496
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2328..2348)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2513..2648
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2697..3491
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2697..3491
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2751..2770)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3361..3380
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3679..3698)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3723..3770
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4099..4687
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4588..4607
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
61 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
121 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
181 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
241 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
301 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
361 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
421 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
481 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
541 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
601 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc
661 tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
721 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
781 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
841 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc tgcaccaccg
901 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc ctgcagtgct
961 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
1021 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
1081 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
1141 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
1201 atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
1261 tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
1321 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
1381 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
1441 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
1501 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
1561 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
1621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1681 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
1741 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
1801 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
1861 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
1921 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
1981 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
2041 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
2101 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
2161 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
2221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
2281 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
2341 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2401 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2461 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
2521 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2581 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
2641 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
2701 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
2761 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
2821 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
2881 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
2941 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
3001 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
3061 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
3121 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
3181 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
3241 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
3301 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
3361 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
3421 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
3481 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
3541 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
3601 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
3661 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
3721 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
3781 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
3841 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
3901 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
3961 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
4021 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
4081 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
4141 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
4201 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
4261 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
4321 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
4381 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
4441 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
4501 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4561 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4621 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4681 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggc
//
