pLX313 MSLN

LOCUS       pLX313 MSLN       10711 bp DNA     circular SYN 25-AUG-2024
DEFINITION  Expresses MSLN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Hagel KR, Arafeh R, Gang S, Arnoff TE, Larson RC, Doench JG, 
            Mathewson ND, Wucherpfennig KW, Maus MV, Hahn WC
  TITLE     Systematic interrogation of tumor cell resistance to CAR T cell 
            therapy in pancreatic cancer.
  JOURNAL   Cancer Res. 2022 Dec 22:CAN-22-2245. doi: 
            10.1158/0008-5472.CAN-22-2245.
  PUBMED    36548402
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 7.0.3
            https://www.biofen.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2022 Dec 22:CAN-22-2245. doi: 10.1158/0008-5472.CAN-22-2245."
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1539..1749
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1561..1757
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1608..1743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1779..2798
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    2869..2986
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3124..4302
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3355..4293
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4250..4270
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    4322..4346
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6213..6237)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6245..6286
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6337..6925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6390..6410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6808..6819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6997..7230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7308..7429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7345..7364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7399..7418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7469..7604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7624..7643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7625..7643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7653..7670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7653..7669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7662..7684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7811..8266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7898..7917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8108..8129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8292..8396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8397..9257
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8615..8634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9428..10016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9917..9936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10170..10187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10304..10325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10340..10370
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10378..10394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10383..10405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10402..10418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10402..10418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10437..10457
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10439..10457
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10485..10711
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg catctcaatt
     1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1561 tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1741 gcctaggctt ttgcaaaaag ctttctagag gtaccaccat gaaaaagcct gaactcaccg
     1801 ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     1861 tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc
     1921 tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg
     1981 catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga
     2041 cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac
     2101 tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta
     2161 gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc
     2221 gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     2281 acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact
     2341 gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca
     2401 atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg
     2461 aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct
     2521 acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc
     2581 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     2641 gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac
     2701 aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata
     2761 gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa tgaggcgcgc
     2821 cgttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2881 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2941 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     3001 ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag atggataaag
     3061 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     3121 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     3181 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     3241 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     3301 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     3361 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     3421 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     3481 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     3541 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     3601 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     3661 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     3721 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     3781 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     3841 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     3901 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     3961 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     4021 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     4081 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     4141 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     4201 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     4261 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggctagca tcgattgatc
     4321 aacaagtttg tacaaaaaag ttggcatggc cttgctaacg gctcgacccc tgttggggtc
     4381 ctgtgggacc cccgccctcg gcagcctcct gttcctgctc ttcagcctcg gatgggtgca
     4441 gccctcgagg accctggctg gagagacagg gcaggaggct gcgcccctgg acggagtcct
     4501 ggccaaccca cctaacattt ccagcctctc ccctcgccaa ctccttggct tcccgtgtgc
     4561 ggaggtgtcc ggcctgagca cggagcgtgt ccgggagctg gctgtggcct tggcacagaa
     4621 gaatgtcaag ctctcaacag agcagctgcg ctgtctggct caccggctct ctgagccccc
     4681 cgaggacctg gacgccctcc cattggacct gctgctattc ctcaacccag atgcgttctc
     4741 ggggccccag gcctgcaccc gtttcttctc ccgcatcacg aaggccaatg tggacctgct
     4801 cccgaggggg gctcccgagc gacagcggct gctgcctgcg gctctggcct gctggggtgt
     4861 gcgggggtct ctgctgagcg aggctgatgt gcgggctctg ggaggcctgg cttgcgacct
     4921 gcctgggcgc tttgtggccg agtcggccga agtgctgcta ccccggctgg tgagctgccc
     4981 gggacccctg gaccaggacc agcaggaggc agccagggcg gctctgcagg gcgggggacc
     5041 cccctacggc cccccgtcga catggtctgt ctccacgatg gacgctctgc ggggcctgct
     5101 gcccgtgctg ggccagccca tcatccgcag catcccgcag ggcatcgtgg ccgcgtggcg
     5161 gcaacgctcc tctcgggacc catcctggcg gcagcctgaa cggaccatcc tccggccgcg
     5221 gttccggcgg gaagtggaga agacagcctg tccttcaggc aagaaggctc ccgagataga
     5281 cgagagcctc atcttctaca agaagtggga gctggaagcc tgcgtggatg cggccctgct
     5341 ggccacccag atggaccgcg tgaacgccat ccccttcacc tacgagcagc tggacgtcct
     5401 aaagcataaa ctggatgagc tctacccaca aggttacccc gagtctgtga tccagcacct
     5461 gggctacctc ttcctcaaga tgagccctga ggacattcgc aagtggaatg tgacgtccct
     5521 ggagaccctg aaggctttgc ttgaagtcaa caaagggcac gaaatgagtc ctcaggtggc
     5581 caccctgatc gaccgctttg tgaagggaag gggccagcta gacaaagaca ccctagacac
     5641 cctgaccgcc ttctaccctg ggtacctgtg ctccctcagc cccgaggagc tgagctccgt
     5701 gccccccagc agcatctggg cggtcaggcc ccaggacctg gacacgtgtg acccaaggca
     5761 gctggacgtc ctctatccca aggcccgcct tgctttccag aacatgaacg ggtccgaata
     5821 cttcgtgaag atccagtcct tcctgggtgg ggcccccacg gaggatttga aggcgctcag
     5881 tcagcagaat gtgagcatgg acttggccac gttcatgaag ctgcggacgg atgcggtgct
     5941 gccgttgact gtggctgagg tgcagaaact tctgggaccc cacgtggagg gcctgaaggc
     6001 ggaggagcgg caccgcccgg tgcgggactg gatcctacgg cagcggcagg acgacctgga
     6061 cacgctgggg ctggggctac agggcggcat ccccaacggc tacctggtcc tagacctcag
     6121 catgcaagag gccctctcgg ggacgccctg cctcctagga cctggacctg ttctcaccgt
     6181 cctggcactg ctcctagcca ccaccctggc ctgcccaact ttcttgtaca aagtggttga
     6241 tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag
     6301 taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     6361 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6421 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6481 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6541 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6601 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6661 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6721 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6781 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6841 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6901 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     6961 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7021 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7081 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7141 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7201 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     7261 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     7321 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7381 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     7441 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7501 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7561 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     7621 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7681 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     7741 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     7801 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7861 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     7921 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     7981 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8041 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8101 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8161 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8221 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     8281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     8401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     8461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     8521 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8941 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     9121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     9181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     9241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     9301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     9361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9541 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     9601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    10141 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    10201 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    10261 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    10321 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    10381 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    10441 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
    10501 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
    10561 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
    10621 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
    10681 tgtatttaag tgcctagctc gatacataaa c
//