pLKO.1 neo

LOCUS       pLKO.1 neo Sequencing Result	7589 bp 	DNA	SYN
DEFINITION  pLKO.1 neo Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="pLKO.1 neo Sequencing Result"
                     /mol_type="other DNA"
     promoter        742..791
                     /label="NEOKAN_promoter"
     CDS             880..1674
                     /label="ORF frame 1"
     gene            883..1671
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     CDS             complement(1189..1695)
                     /label="ORF frame 3"
     misc_feature    complement(1697..1713)
                     /label="pBluescriptKS_primer"
     misc_feature    1778..1799
                     /label="U3PPT"
     misc_feature    1778..1793
                     /label="cPPT"
     misc_feature    1795..1847
                     /label="delta_U3"
     misc_feature    1848..2028
                     /label="HIV-1_5_LTR"
     misc_feature    1848..2028
                     /label="truncHIV-1_3_LTR"
     terminator      2103..2222
                     /label="SV40_PA_terminator"
     misc_feature    2191..2210
                     /label="EBV_rev_primer"
     rep_origin      2240..2338
                     /label="SV40_origin"
     misc_feature    2323..2342
                     /label="SV40pro_F_primer"
     promoter        complement(2417..2435)
                     /label="T7_promoter"
     promoter        complement(2445..2461)
                     /label="M13_forward20_primer"
     misc_feature    complement(2454..2476)
                     /label="M13_pUC_fwd_primer"
     misc_feature    2454..2602
                     /label="lacZ_a"
     rep_origin      2620..2926
                     /label="f1_origin"
     promoter        3119..3147
                     /label="AmpR_promoter"
     gene            3189..4049
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             3189..4049
                     /label="ORF frame 3"
     rep_origin      4204..4823
                     /label="pBR322_origin"
     promoter        5132..5161
                     /label="lac_promoter"
     misc_feature    5175..5197
                     /label="M13_pUC_rev_primer"
     promoter        5196..5214
                     /label="M13_reverse_primer"
     promoter        5231..5250
                     /label="T3_promoter"
     misc_feature    5504..5684
                     /label="truncHIV-1_3_LTR"
     misc_feature    5504..5684
                     /label="HIV-1_5_LTR"
     misc_feature    5795..5839
                     /label="HIV-1_psi_pack"
     CDS             6227..7084
                     /label="ORF frame 2"
     misc_feature    6349..6582
                     /label="RRE"
     promoter        7118..7350
                     /label="hU6_promoter"
     misc_feature    7280..7299
                     /label="LKO1_5_primer"
     misc_feature    7424..7439
                     /label="cPPT"
ORIGIN
    1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
   61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
  121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
  181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
  241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
  301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
  361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
  421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
  481 gaccgaatca ccgacctctc tccccagggg gatccaccgg aTCAAGCTTC ACGCTGCCGC
  541 AAGCACTCAG GGCGCAAGGG CTGCTAAAGG AAGCGGAACA CGTAGAAAGC CAGTCCGCAG
  601 AAACGGTGCT GACCCCGGAT GAATGTCAGC TACTGGGCTA TCTGGACAAG GGAAAACGCA
  661 AGCGCAAAGA GAAAGCAGGT AGCTTGCAGT GGGCTTACAT GGCGATAGCT AGACTGGGCG
  721 GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG TAAGGTTGGG
  781 AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG GCGCAGGGGA
  841 TCAAGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
  901 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
  961 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
 1021 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
 1081 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
 1141 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
 1201 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
 1261 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
 1321 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCaGGG GCTCGCGCCA
 1381 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
 1441 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCtTTTC TGGATTCATC
 1501 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
 1561 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
 1621 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
 1681 CTCTGGGGTT CGCATCGATA CCGTCGACCT CGAGGGGGGG CCcggtacct ttaagaccaa
 1741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
 1801 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
 1861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
 1921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
 1981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
 2041 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
 2101 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
 2161 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
 2221 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
 2281 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
 2341 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
 2401 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
 2461 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
 2521 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
 2581 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
 2641 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
 2701 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
 2761 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
 2821 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
 2881 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
 2941 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
 3001 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
 3061 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
 3121 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
 3181 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
 3241 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
 3301 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
 3361 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
 3421 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
 3481 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
 3541 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
 3601 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
 3661 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
 3721 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
 3781 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
 3841 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
 3901 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
 3961 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
 4021 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
 4081 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
 4141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
 4201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
 4261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
 4321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
 4381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
 4441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
 4501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
 4561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
 4621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
 4681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
 4741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
 4801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
 4861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
 4921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
 4981 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
 5041 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
 5101 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
 5161 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
 5221 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
 5281 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
 5341 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
 5401 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
 5461 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
 5521 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
 5581 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
 5641 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
 5701 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
 5761 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
 5821 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
 5881 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
 5941 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
 6001 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
 6061 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
 6121 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
 6181 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
 6241 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
 6301 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
 6361 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
 6421 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
 6481 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
 6541 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
 6601 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
 6661 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
 6721 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
 6781 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
 6841 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
 6901 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
 6961 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
 7021 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
 7081 ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
 7141 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
 7201 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
 7261 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
 7321 cttggcttta tatatcttgt ggaaaggacg aaacaccggt CCGCAGGTAT GCACGCGTga
 7381 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
 7441 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
 7501 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
 7561 agatccactt tggccgcggc tcgaggggg
//