| 质粒名称: | pJK-caCas9-NatMX-Neut5L-Ade2 drive |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 89577 |
| 存储实验室: | George Church |
|
载体骨架基本信息 |
|
| 载体名称: | pJK1027 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 插入caCas9和Neut5L同源臂 |
| 载体类型: | 酵母表达，合成生物学 |
| 筛选标记: | Nourseothricin |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Ade2 gene drive |
| 物种来源: | C. albicans |
| 插入大小: | 1000 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | TTGCAATTATTAGAGATCCAGAAAA |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJK-caCas9-NatMX-Neut5L-Ade2 drive was a gift from George Church (Addgene plasmid # 89577 ; http://n2t.net/addgene:89577 ; RRID:Addgene_89577) |
| 参考文献部分: | A CRISPR-Cas9-based gene drive platform for genetic interaction analysis in Candida albicans. Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE, Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins JJ. Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0. Epub 2017 Oct 23. 10.1038/s41564-017-0043-0 PubMed 29062088 |
买家导航
pJK-caCas9-NatMX-Neut5L-Ade2 drive载体质粒基本信息
pJK-caCas9-NatMX-Neut5L-Ade2 drive质粒图谱载体图谱和pJK-caCas9-NatMX-Neut5L-Ade2 drive载体序列质粒序列多克隆位点信息
pJK-caCas9-NatMX-Neut5L-Ade2 drive质粒载体简介
pJK-caCas9-NatMX-Neut5L-Ade2 drive质粒序列载体序列
LOCUS pJK-caCas9-NatMX-Neut5L-Ade2 drive 12969 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION caCas9 integrating vector into the Neut5L locus with gene drive for
targeting Ade2 locus.
ACCESSION .
VERSION .
KEYWORDS pJK-caCas9-NatMX-Neut5L-Ade2 drive
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12969)
AUTHORS Shapiro RS, Chavez A, Porter CBM, Hamblin M, Kaas CS, DiCarlo JE,
Zeng G, Xu X, Revtovich AV, Kirienko NV, Wang Y, Church GM, Collins
JJ
TITLE A CRISPR-Cas9-based gene drive platform for genetic interaction
analysis in Candida albicans.
JOURNAL Nat Microbiol. 2018 Jan;3(1):73-82. doi: 10.1038/s41564-017-0043-0.
Epub 2017 Oct 23.
PUBMED 29062088
REFERENCE 2 (bases 1 to 12969)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12969
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..4104
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 4117..4137
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 4142..4158
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 4143..4159
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
terminator 5095..5292
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
promoter complement(5893..6236)
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
primer_bind complement(7199..7218)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(7200..7218)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(7225..7242)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7225..7241)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7234..7256)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(7470..7489)
/label=F1ori-R
/note="F1 origin, reverse primer"
terminator 7742..7761
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
misc_RNA complement(7766..7840)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(7866..7940)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 9587..9608
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 9771..9875
/gene="bla"
/label=AmpR promoter
CDS 9876..10736
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10094..10113)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 10907..11495
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11396..11415
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 11649..11666
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 11783..11804
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 11819..11849
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11857..11873
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 11862..11884
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 11881..11897
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 11881..11897
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 11918..11936
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 atggataaaa aatactccat aggtttggac attggaacca attcagttgg atgggctgta
61 atcactgatg aatataaggt tccttctaaa aagttcaaag ttttaggaaa tactgataga
121 cactccatta aaaagaattt aattggtgcc ttgttgtttg acagtggtga aacagctgaa
181 gcaactcgtt tgaaaagaac agctagaaga agatatacca gaagaaagaa cagaatttgt
241 tacttgcaag aaatcttttc taatgaaatg gcaaaggttg atgattcttt ttttcataga
301 ttggaagaaa gtttcttagt tgaagaagat aagaaacatg aaagacatcc aatctttggt
361 aatattgtag atgaagtggc ttaccacgaa aaatatccta caatttatca tttgagaaaa
421 aaattagttg actccactga taaagctgac ttaagactta tatatttggc attggctcac
481 atgatcaagt tcagaggtca cttcttaatt gaaggtgatt tgaatccaga taattccgat
541 gtcgacaagt tgtttattca attggttcaa acttacaacc aacttttcga ggaaaatcca
601 atcaatgcct caggtgtgga tgctaaagct attttatctg ctagattgtc aaaaagtaga
661 cgtttggaaa atcttattgc ccaattgcca ggtgaaaaaa aaaatggatt gtttggaaac
721 ttaattgcat tatctttggg tttgacccca aatttcaaat caaactttga tttggccgag
781 gatgctaaac ttcaattgtc taaggataca tacgatgacg atttagataa tcttcttgcc
841 caaattggtg atcaatatgc agatttattc ttggcagcta agaacttgag tgatgcaatt
901 ttgttaagtg acattttgag agtcaacaca gaaattacaa aagctccttt atccgcctct
961 atgattaaaa gatatgatga acaccatcaa gatttaacct tgttgaaagc tcttgtacgt
1021 caacaattac cagaaaagta taaagaaata ttctttgatc aatctaaaaa tggttacgct
1081 ggttatattg atggtggtgc tagtcaagaa gaattttata agtttatcaa gccaattctt
1141 gaaaaaatgg atggtactga ggaattgttg gttaagttga atcgagagga tttacttcgt
1201 aagcaaagaa catttgataa cggttctatc cctcatcaaa tccacttagg tgagttacac
1261 gctatcttga gacgtcagga agatttttac ccttttttga aagacaatcg tgaaaaaatt
1321 gaaaaaattt tgactttcag aattccttat tacgtcgggc cattggctag aggtaattct
1381 agatttgcat ggatgactcg taaatctgaa gagactatca ccccatggaa ttttgaggaa
1441 gttgttgata agggagcctc cgctcaatca ttcatcgaga gaatgaccaa tttcgacaaa
1501 aacttgccaa atgagaaggt gttacctaaa cattctttgt tatatgaata tttcactgtt
1561 tataatgaat taacaaaagt caaatacgtc actgaaggta tgagaaaacc agcattctta
1621 agtggtgaac aaaagaaagc tatcgttgac ttgttattca aaactaatcg taaagtcact
1681 gtcaaacaac ttaaagagga ctattttaag aaaatagagt gtttcgattc cgttgaaatc
1741 agtggggttg aggatagatt taatgctagt ttaggaactt atcacgattt gttgaaaatt
1801 atcaaagaca aagatttttt ggataatgaa gaaaacgaag atatcttgga ggacattgta
1861 ttgactttga ctttgtttga ggatagagaa atgatcgaag aacgtttaaa aacttacgct
1921 cacttattcg acgataaagt catgaaacaa ttaaaaagaa gaagatatac cggttgggga
1981 agattatcta gaaagttgat taatggtatt agagataagc aaagtggtaa aaccatctta
2041 gatttcctta aaagtgatgg ttttgcaaac agaaacttta tgcagttgat tcatgatgat
2101 agtttgacct ttaaggaaga tattcaaaaa gctcaggttt ctggacaggg tgattcattg
2161 catgaacata ttgccaactt agcagggtct ccagctatca aaaagggaat tttacaaact
2221 gttaaagttg ttgatgagtt ggtgaaagtt atgggacgtc ataagcctga aaatatagtt
2281 atagaaatgg ctagagaaaa tcaaacaact cagaaaggac aaaagaattc tcgtgaaaga
2341 atgaaaagaa tagaagaagg aattaaagag ttgggttctc aaatcttaaa agaacaccca
2401 gtcgaaaaca cccagttaca gaacgagaag ctttacttgt actatttgca aaacgggcgt
2461 gacatgtatg tagatcaaga gttggatatc aatagacttt cagattacga tgtcgatcac
2521 atagttcctc agtcattttt aaaagatgat tccattgaca acaaagtttt aacaagatct
2581 gataagaata gaggtaaatc agacaatgtg ccatcagaag aagtggttaa gaaaatgaag
2641 aactattgga gacaattgtt aaatgcaaag ttgattactc agagaaaatt tgacaactta
2701 actaaagctg aaagaggagg attatccgaa ttagataaag ctggattcat aaaacgtcag
2761 ttggttgaaa caagacaaat tactaaacat gtagcccaaa ttttagattc ccgaatgaat
2821 actaaatatg atgaaaatga caaattaatc agagaggtca aagtgatcac attaaagtca
2881 aagttggtgt cagatttccg aaaggatttt caattctaca aggtgagaga gatcaacaat
2941 tatcatcatg ctcacgacgc atatttgaat gcagtggtag gaactgcctt aataaagaag
3001 tatcctaagt tagaatccga attcgtgtat ggtgattata aagtctacga tgtcagaaag
3061 atgatagcaa aatcagaaca agaaattggg aaagccaccg caaagtattt cttttactcc
3121 aacatcatga atttctttaa gaccgaaatc accttggcta acggggaaat cagaaaacgt
3181 cctttgattg aaacaaatgg agaaaccggt gaaattgtgt gggataaggg gagagatttt
3241 gctactgtta gaaaagtttt atcaatgcct caagttaata ttgtaaagaa gactgaagtt
3301 caaactggtg gtttctctaa ggaatccatt cttccaaaac gaaactcaga caagttgatc
3361 gcccgaaaaa aagattggga tcctaagaaa tacggaggtt ttgattctcc tactgtcgca
3421 tattctgtct tggtcgtggc taaagttgaa aagggtaaat ccaaaaaatt gaaatctgtg
3481 aaagaattgt taggtattac catcatggag cgttcatcct ttgagaaaaa cccaattgac
3541 tttctcgaag cgaagggcta taaagaagtg aagaaagatt tgattattaa gttgccaaag
3601 tattctctct ttgaacttga aaatgggaga aagagaatgt tggcttcagc tggagaattg
3661 caaaaaggaa acgagttagc tttgccttca aagtacgtta atttcttata cttagcatct
3721 cattatgaaa agttgaaggg gtcccctgaa gacaatgagc aaaagcaatt attcgtagaa
3781 caacataaac actatttgga tgagataatt gaacaaatct ccgagttttc taaaagagtt
3841 attttggctg atgcaaattt ggataaagtt ttatctgctt ataataagca tcgtgacaaa
3901 ccaattcgag aacaagctga aaatattatc cacttgttca cattgactaa cttgggagct
3961 ccagccgcgt ttaagtactt tgacaccacc attgaccgta agagatatac ttcaacaaaa
4021 gaagtgttag acgccacatt gattcatcag tccataactg gtttatatga aactagaatc
4081 gatttgtcac aattgggggg tgatagccga gcggatccta agaaaaaacg aaaagtctga
4141 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccgag
4201 tgaaattctg gaaatctgga aatctggttt tgtattcttg ttattcttct ttttgttatt
4261 acatatataa cttgttactt ttttaaaaaa atctttgttt attttataaa tatataaaac
4321 taaatttaag aaaaagagaa aaatgtttta tttgagagat tgaaatttta cttgaattta
4381 gcttagcttt tataaagtat tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt
4441 aagactataa aatagccacc caaggcattt ctatatcttg ttgttgttgt tttcatcttc
4501 tgtatcagag gaacttattt tattattttc gtcacgggta ttttctcttg tttgatgatt
4561 catcccattc attccatcat aaaattctag aaggaccacc tttgattgta aatagtaata
4621 attaccaccc ttatttaatt atttatttaa cttatttatt tatttattat acatatatac
4681 aaatctaata aagtgaaaat ctcccccttc acatttataa ttggccagtt tttttcaaat
4741 aagcattcca accagcatct ctataccttt taccttcaat atctggatct cttcctttac
4801 caaacaatcc tctaccaaca atgataatat cagttccagt gctaacaact tcatcaacag
4861 ttctatattg ttgtcctaat ccatcacctt tatcatctaa tccaactcca ggtgtcataa
4921 taagccaatc aaatccttct tcttgtccac ccatatcacg ttgggcaata aatccaataa
4981 caaattcctt atcggattta gcaatttcaa cagttttttg agaatattct ccatacgaat
5041 tcgagctcgt ttaaacgtcg acactggatg gcggcgttag tatcgaatcg acagcagtat
5101 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
5161 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
5221 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
5281 tttattgtca gtactgactc gagttattat ggacatggca tagacatata caaagcttgt
5341 tcaccatcgg aagcagtacc atcgtataaa gcagtatcca aaccacacaa agtgaaaccc
5401 attcttctat aagcatgaat agctggagca ttaacattgg taacttccaa ccacaaatga
5461 ccagcacctc tttctctggc gaattcagta gccaaaccca tcaaagctct accaacacca
5521 tgacctctat gttctggagc aacttcaata tcttcaacag tcaatcttct gttccaacca
5581 gaataagaaa caacaacgaa accagccaaa tcaccatcat caccataagc aacgaaagtt
5641 ctagaatctg gatcaccatc ttcaccagca tcggattcat catcggattc atcatctggg
5701 aaaaccttag tcaatggtgg atcaactgga acttctctca aagtgaaacc atcaccagta
5761 gcagtaactc taaaaacagt atcggtagtg aaagaaccat ccaaagcttc aatagcttca
5821 gcatcacctg gaacagaagt tctgtatcta taagcagtat catccaaagt agtagacata
5881 attgtaggat ccggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt
5941 ttaaaaggaa gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc
6001 tacaggggcg cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg
6061 caggtctgca gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg
6121 gatgcaaatg attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat
6181 gcccctgagc tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg
6241 gccgggtgac ccggcgggga cgaggcaagc ttgatatgct aatgatccca ctgatgataa
6301 ttcagctaac atcaataacc ctcttggctc ttggttggtg gtggtttctt tagctccctg
6361 ttttaatcct tcaactactc cattcccagt gacaccatga gcattagtaa tatctgccca
6421 actactaatt ttataaactc caccaatata ttgtttcttc acggtattac caatatcagc
6481 aaattttcta tcttcaaaaa tcataaattg atgtttacgt gaaagttcta ataatggttc
6541 aatagtggat tcataggaaa aatcattgat tatatcaata tgagtcttga ttaagcatac
6601 ataaggaccc aatttatcaa ttaattcaag gaattcctta gtggtatcaa catcaattga
6661 tgcacataaa ttggttttct tcagttccat taatcgaaat aatcgttgtg ctactggtga
6721 ggcatgagtt tctgctctct cactataggt cttagtgttg actgtcatat cttttgtttg
6781 ctatttctat ttctatttct ttcttcaact gtaaaagggg cgatatataa accagtgtgt
6841 atggggttgt tgctcaggaa ttgatcaatg tttaaaagaa tggttgaaat catcattgaa
6901 gaacaaaaaa aaagattttt tctttcatta tgttatctgt ataatatgca ggaaataaga
6961 ttgcaaaaaa atagccgttt ttttggatac tatcaaacaa gaggaatgac taatacagaa
7021 ttagtgttac gaatcaatgg cactacagca actttcaaca aaataatcta cgttcaaaat
7081 ataatatata ctattaaata aaatcaaata ataaaataat tttaaagaaa gtagtaccag
7141 ctcttttttt tgtttccgtt tataccatcc aaatcaattc ctattagtct ccaattcgcc
7201 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
7261 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
7321 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
7381 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
7441 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
7501 cacgttcgcc tttaaatgat aattatagta ttcacggata gatctgttag agatgtggtg
7561 gattggtatt tctttctgtg attcaccgaa agtattatca actaagaagg gaaaggcacc
7621 acattttcta atgtctcata tattagttca gttcgatgta tttcttttta tatattaatt
7681 ataattttat tgcaccataa cgtttacttg tttaatatgc tattgatatc tatatgcggc
7741 cagacataaa aaacaaaaaa agcaccaccg actcggtgcc actttttcaa gttgataacg
7801 gactagcctt attttaactt gctatttcta gctctaaaac attaggtcgt atgattgttg
7861 cgcaccaccg actcggtgcc actttttcaa gttgataacg gactagcctt attttaactt
7921 gctatttcta gctctaaaac atttcttcta aagtttctgc caaattaaaa atagtttacg
7981 caagtctcga acttccacca caatttaatt gtaagaacgc tgcgaaaaca tattctccaa
8041 tcctattggg ccgaagtttc agctatgcca aaggcatcaa attttttttt ataaaatttt
8101 ccacttttcc ataccagtga aattatcacg tgatggagtg agtatctata atatttgatt
8161 gtttctttga tgaggcaata ctcgagtata gttattgacc aatgactttt agctatgtgt
8221 agtgcttgta tatgcgtgtg tgatgagaat aagatgaata gattagtaat gtataagttg
8281 attctttttg gtggggggtg ttacttttat tttttctttc catctcattg acaagattgt
8341 accgagtcaa aatgtttggg gtctttttat tgctgcgatg gattgatttt tcactctaag
8401 cctaaaatta aatggctaaa caagtggtat tcaagcacaa ttcagttttc tggatctcta
8461 ataattgcaa ttgcaattgc ttcacatact ctgtttataa ttcagtttct ttctcttgat
8521 gttactgata tattaagtta ataaatacca ataacttttt ttcaaaaatc ttctattttc
8581 ctttcaattg attatttaaa cacgaatcgt taataagctg tgattgcttt ttctctttct
8641 ttttcttttc tttgttcaag tttgaaaaaa aaaaaaaaag gtttctcttg ttcgatgtag
8701 aattcagtgg gttatagttg taatgatata tggaataaaa agaaagaaag agaagagaaa
8761 gagaagaaga gaagaattaa gtaaacgtgt attagaattt aaaaattaaa attcatgata
8821 atctttaata ataattaact aagaatcaca aaattgaaaa tttgaaagaa aaaagatcac
8881 tccaaggtcg tgtcaagtga ctagaaattc ttaattaaaa tagttataac aaaaaaagaa
8941 atttgtatat aacaattaag taaaagaaaa aaaagcaatt cgctttgtat aataataccc
9001 agaaaaatat attcatatat tcataaatca aacatgaaat gaagaatgct gaatcacttg
9061 ataggattta gttccattat ggtactaaga agtaatatta cttctatgaa aaggttgaat
9121 ccgagatcaa aattcatctt ttcatatact ctggagaaaa aaaaaaaagt aattgtagta
9181 agaatgacaa gtatcagttg agaaaaaaag taacaaagag aaagctcgga ggaggctccc
9241 caaaggtttt atcaccaatg gtggtaccac taacccaaga acagaaaaag cattgatatt
9301 ttgaaaaata caatactcat cttttaaccc aaaccttttc ttgtccttta aaacttttat
9361 ctcatgtctg tccaaagtca tattgtctta ctactttctc tccttcaacg tccttccggc
9421 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
9481 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
9541 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
9601 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
9661 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
9721 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
9781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
9841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
9901 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
9961 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
10021 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
10081 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
10141 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
10201 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
10261 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
10321 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
10381 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
10441 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
10501 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
10561 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
10621 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
10681 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
10741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
10801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
10861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
10921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
10981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
11041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
11101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
11161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
11221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
11281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
11341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
11401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
11461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
11521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
11581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
11641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
11701 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
11761 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
11821 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
11881 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
11941 aaaagctggt accagagcta ttaagatcac cagcctcgtt tataataaac ttagtctaat
12001 atttcttttg gaccaaaata gaaactgttc tcaggtggca aaaaacagct taaagtctgt
12061 gttaaaaaaa gatctcggct atgccaatca aaaaggagtg acattattat gtaaaagaag
12121 aaatgttgta attccgcgaa acaacaattt taatgcttac tgtgccgcca ccaatatcct
12181 atggccaagg ggcctttaaa catgccacgg aaatatacag tcaaagtaaa cctactatgt
12241 ctttagagcc ttcaggatca attgttttta aatgaatgac tgcaaaacta gaaaagtagc
12301 ctcttacata agcaagtcat ttgtacaccg agagtttagt aaccagtgaa gcaagtatat
12361 ctggcaaacc tgaaacttat ctgtgaatta taaagctcat aagtaaaaag tgtagaaaag
12421 aagataaggc agaccaaata caaatttgca acagtacaac gaatatttca gcgtcaaaac
12481 tagagaataa taaagaaaac gatcttttca aaaagaaaaa accttttagt tttcctttgt
12541 tgttgttgtg ggtgtgtgct atttatatta tatagtttac tcataatacc ataaaatatt
12601 cggtttgatt aggttatttt aataagctaa tttgtttcta atcgtgtaat ttatgctgtg
12661 tatattaagt agtgtgtgca ctgcccaaaa atgtttgttg tttatagtcg gttaaagaga
12721 aaaaagaaaa aaagatccat acacacacgt taattagttg ttcaacgtaa tacactcata
12781 ttttgttctt atttgctttc ggtcgctgtt ctcaccaaga tttattgcca acgaaacaat
12841 ttttttttat atattttcag atttttcttt ttttcctttc ctttcctttt ctaattttca
12901 ctcctggttt tctttctttc ttagaaacat tatctcgata ttaatattaa aaaaatataa
12961 tcattcaaa
//
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