| 质粒名称: | pGreenIIM DR5v2-ntdTomato/DR5-n3GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 61628 |
| 存储实验室: | Dolf Weijers |
|
载体骨架基本信息 |
|
| 载体名称: | pGreenII |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 2580 bp |
| 完整质粒大小: | 9692 bp |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | Methothrexate |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ntdTomato |
| 物种来源: | Synthetic |
| 插入大小: | 1518 bp |
| 基因突变: | -- |
| GenBank编号 | KJ081243.1 |
| 启动子 | Auxin-dependent DR5v2 promoter containing 9 reverse TGTCGG sites and -46 35S promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII（如果被摧毁则未知） |
| 3' 克隆位点: | XhoI（如果被摧毁则未知） |
| 5' 测序引物 | M13_pUC_fwd |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGreenIIM DR5v2-ntdTomato/DR5-n3GFP was a gift from Dolf Weijers (Addgene plasmid # 61628 ; http://n2t.net/addgene:61628 ; RRID:Addgene_61628) |
| 参考文献部分: | Reporters for sensitive and quantitative measurement of auxin response. Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D. Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub 2015 Feb 2. 10.1038/nmeth.3279 PubMed 25643149 |
买家导航
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP载体质粒基本信息
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP质粒图谱载体图谱和pGreenIIM DR5v2-ntdTomato/DR5-n3GFP载体序列质粒序列多克隆位点信息
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP质粒载体简介
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP质粒序列载体序列
LOCUS pGreenIIM DR5v2-ntdTomato/DR5-n3GFP 9680 bp ds-DNA circular SYN 01-OCT-2019
DEFINITION Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG)
promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter.
ACCESSION .
VERSION .
KEYWORDS pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9680)
AUTHORS Liao CY, Smet W, Brunoud G, Yoshida S, Vernoux T, Weijers D
TITLE Reporters for sensitive and quantitative measurement of auxin
response.
JOURNAL Nat Methods. 2015 Mar;12(3):207-10. doi: 10.1038/nmeth.3279. Epub
2015 Feb 2.
PUBMED 25643149
REFERENCE 2 (bases 1 to 9680)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 1, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9680
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(607..635)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 1230..1250
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind complement(1634..1654)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1634..1652)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(1673..1689)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1673..1689)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1686..1708)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1697..1713
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1721..1751)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1766..1787
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1904..1921)
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2026..2050
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin complement(2141..2729)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2221..2240)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(2900..3715)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 3623..3642
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 3707..3726
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 4006..4441
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
misc_feature 4568..4590
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA
(truncated)"
polyA_signal 4598..4774
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(5170..5733)
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/label=DHFR
/translation="MVRPLNCIVAVSQNMGIGKNGDRPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKKD"
promoter complement(5766..6111)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(5803..5823)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
primer_bind 6314..6336
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6328..6345
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6329..6345
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6355..6374
/label=T7
/note="T7 promoter, forward primer"
promoter 6355..6373
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 6602..6647
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 6658..6713
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 6760..6780
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6781..8211
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/label=tdTomato
/note="mammalian codon-optimized"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
promoter 8637..8682
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 8693..8748
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 8770..8790
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8791..9507
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 9514..553
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
ORIGIN
1 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
61 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
301 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
541 gctgtacaag taaagatcca aggagatata accatgagta gtaaaggaga agaacttttc
601 actggagttg tcccaattct tgttgaatta gatggtgatg gtaatgggca caaattttct
661 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
721 actactggaa aactacctgt tccatggcca acacttgtca ctacgttaac ttatggagta
781 caatgctttt caagataccc agatcatatg aagcggcacg acttcttcaa gagcgccatg
841 cctgagggat acgtgcagga gaggaccatc ttcttcaagg acgacgggaa ctacaagaca
901 cgtgctgaag tcaagtttga gggagacacc ctcgtcaaca ggatcgagct taagggaatc
961 gatttcaagg aggacggaaa catcctcggc cacaagttgg aatacaacta caactcccac
1021 aacgtataca tcatggcaga caaacaaaag aatggaatca aagtcaactt caaaattaga
1081 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
1141 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
1201 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
1261 attacacatg gcatggatga atacaaatct tacgttactg ttccaaacag aactagaact
1321 tacatcaagt aatctagatc cgatccccga tcgttcaaac atttggcaat aaagtttctt
1381 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
1441 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
1501 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
1561 ggataaatta tcgcgcgcgg tgtcatctat gtctagagcg gccgccaccg cggtggagct
1621 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
1681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
1741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
1801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
1861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
1921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
1981 tatccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca ggatatattg
2041 gcgggtaaac taagtcgctg tatgtgtttg tttgagatct catgtgagca aaaggccagc
2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaga agagttggta
2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
2881 tgtaacattg gtctagtgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
2941 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
3001 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
3061 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
3121 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
3181 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
3241 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
3301 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
3361 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag
3421 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
3481 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
3541 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cggtagattg
3601 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
3661 tgttggaatt taatcgcggc cttgagcaag acgtttcccg ttgaatatgg ctcataacac
3721 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
3781 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
3841 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
3901 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
3961 gctccctcac tttctggctg gatgatgggg cgattcaggc gatccccatc caacagcccg
4021 ccgtcgagcg ggctttttta tccccggaag cctgtggata gagggtagtt atccacgtga
4081 aaccgctaat gccccgcaaa gccttgattc acggggcttt ccggcccgct ccaaaaacta
4141 tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa tcggagtacg tgaaatcgct
4201 aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga gaacgctaat agccctttca
4261 gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt tacagcaagt agtatgttca
4321 attagctttt caattatgaa tatatatatc aattattggt cgcccttggc ttgtggacaa
4381 tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag cggttgccca ccgtcgagcg
4441 cctttgccca caacccggcg gccggccgca acagatcgtt ttataaattt ttttttttga
4501 aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct ggatttccga tccccggaat
4561 tagatcttgg caggatatat tgtggtgtaa cgttatcgat ctggatttta gtactggatt
4621 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact
4681 aagggtttct tatatgctca acacatgagc gaaaccctat aagaacccta atttccctta
4741 tcgggaaact actcacacat tatttatgga gaaaatagag agagatagat ttgtagagag
4801 agactggtga tttcagcgta ccgaattaat tcgagctcgg tacccctgga ttttggtttt
4861 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
4921 cttatatgct caacacatga gcgaaaccct ataagaaccc taattccctt atctgggaac
4981 tactcacaca ttattataga gagagataga tttgtagaga gagactggtg atttcagcgg
5041 gggattcagc gccgacggga cgtaaacaaa ggacgtcccg cgaaggatct aaagccagca
5101 aaagtcccat ggtcttataa aaatgcatag ctttaggagg ggagcagaga acttgaaagc
5161 atcttcctgt tagtctttct tctcgtagac ttcaaactta tacttgatgc ctttttcctc
5221 ctggacctca gagaggacgc ctgggtattc tgggagaagt ttatatttcc ccaaatcaat
5281 ttctgggaaa aacgtgtcac tttcaaattc ctgcatgatc cttgtcacaa agagtctgag
5341 gtggcctggt tgattcatgg cttcctggta aacagaactg cctccgacta tccaaaccat
5401 gtctacttta cttgccaatt ccggttgttc aataagtctt aaggcatcat ccaaactttt
5461 ggcaagaaaa tgagctcctc gtggtggttc tttgagttct ctactgagaa ctatattaat
5521 tctgtccttt aaaggtcgat tcttctcagg aatggagaac caggttttcc tacccataat
5581 caccagattc tgtttacctt ccactgaaga ggttgtggtc attctttgga agtacttgaa
5641 ctcgttcctg agcggaggcc agggtcggtc tccgttcttg ccaatcccca tattttggga
5701 cacggcgacg atgcagttca atggtcgaac catgatggca aattctaggg gtacccagct
5761 tgggctgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
5821 tagtgggatt gtgcgtcatc ccttacgtca gtggagatgt cacatcaatc cacttgcttt
5881 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
5941 ttgggaccac tgtcggcaga ggcatcttga atgatagcct ttcctttatc gcaatgatgg
6001 catttgtagg agccaccttc cttttctact gtcctttcga tgaagtgaca gatagctggg
6061 caatggaatc cgaggaggtt tcccgaaatt accctttgtt gaaaagtctc aatagccctt
6121 tggtcttctg agactgtatc tttgacattt ttggagtagg ggtacgataa cattaacgct
6181 tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
6241 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
6301 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
6361 gactcactat agggcgaatt gggtaccggg cccgtatggt taattaagac acaggcgcgc
6421 ctagttggaa taggatttcg aattcccgac aaaaggccga caaaaggccg acaaaaggcc
6481 gacaaaaggc cgacaaaagg ccgacaaaag gccgacaaaa ggccgacaaa aggccgacaa
6541 aaggccgaca aaagggggca ggcctcgata agcttgatat cgaattaatt cctgcagccc
6601 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggtat ttttacaaca
6661 attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat tacaattaca
6721 ggggatccga aatccaactc catactgaag cttatggctc caaagaagaa gagaaaggtc
6781 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
6841 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
6901 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
6961 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
7021 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
7081 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
7141 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
7201 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
7261 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
7321 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
7381 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
7441 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
7501 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
7561 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
7621 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
7681 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
7741 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
7801 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
7861 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
7921 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
7981 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
8041 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
8101 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
8161 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagta gctcgagaga
8221 tccgatcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
8281 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
8341 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
8401 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
8461 ggtgtcatct atgtgaattc gagacaaaag ggagacaaaa gggagacaaa agggagacaa
8521 aagggagaca aaagggagac aaaagggaga caaaagggag acaaaaggga gacaaaaggg
8581 agacaaaagg gggcaggcct cgataagctt gatatcgaat taattcctgc agccccgcaa
8641 gacccttcct ctatataagg aagttcattt catttggaga ggtattttta caacaattac
8701 caacaacaac aaacaacaaa caacattaca attactattt acaattacaa ttacagggga
8761 tccatggctc caaagaagaa gagaaaggtc atggtgagca agggcgagga gctgttcacc
8821 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
8881 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
8941 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
9001 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
9061 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
9121 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
9181 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
9241 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
9301 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
9361 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
9421 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
9481 actctcggca tggacgagct gtacaagaga tccatggtga gcaagggcga ggagctgttc
9541 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
9601 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
9661 accaccggca agctgcccgt
//
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP载体图谱质粒图谱pdf版和pGreenIIM DR5v2-ntdTomato/DR5-n3GFP载体序列质粒序列等相关资料下载
pGreenIIM DR5v2-ntdTomato/DR5-n3GFP质粒载体应用举例
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