| 质粒名称: | pDae079 (eMutaT7[transition] |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 187622 |
| 存储实验室: | Seokhee Kim |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD33 |
| 载体出品公司: | -- |
| 空载体大小: | 5814 bp |
| 完整质粒大小: | 12653 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氯霉素，25μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | TadA-8e-T7RNAP |
| 物种来源: | Synthetic |
| 插入大小: | 3198 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | 5’-ggatcatttgcgcttcagcc-3’ |
| 3’测序引物 | 5'-gagtatgaaaagtctgccgcc-3' |
|
文献引用方法 |
|
| 材料和方法部分: | pDae079 (eMutaT7[transition]) was a gift from Seokhee Kim (Addgene plasmid # 187622 ; http://n2t.net/addgene:187622 ; RRID:Addgene_187622) |
| 参考文献部分: | A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo. Seo D, Koh B, Eom GE, Kim HW, Kim S. Nucleic Acids Res. 2023 Apr 18:gkad266. doi: 10.1093/nar/gkad266. 10.1093/nar/gkad266 PubMed 37070179 |
买家导航
pDae079 (eMutaT7[transition]载体质粒基本信息
pDae079 (eMutaT7[transition]质粒图谱载体图谱和pDae079 (eMutaT7[transition]载体序列质粒序列多克隆位点信息
![pDae079 (eMutaT7[transition]质粒图谱](/uploads/载体图谱/pDae079 (eMutaT7[transition]质粒图谱.png)
pDae079 (eMutaT7[transition]质粒载体简介
pDae079 (eMutaT7[transition]质粒序列载体序列
LOCUS pDae079 (eMutaT7[transition] 12662 bp ds-DNA circular SYN 02-AUG-2022
DEFINITION Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing
genes downstream from a T7 promoter in e.coli.
ACCESSION .
VERSION .
KEYWORDS pDae079 (eMutaT7[transition])
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12662)
AUTHORS Seo D, Eom G, Kim HW, Kim S
TITLE A dual gene-specific mutator system installs all transition
mutations at similar rates in vivo
JOURNAL bioRxiv June 2022
REFERENCE 2 (bases 1 to 12662)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 2, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12662
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(96..974)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
promoter 1001..1285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
promoter 4673..4957
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 8403..8657
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
terminator 8866..8952
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 9044..9071
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 9090..9181
/gene="bla"
/label=AmpR promoter
rep_origin 9639..10094
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9726..9745)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9936..9957
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(10650..11309)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 11224..11243
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(11310..11412)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(11804..11821)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11938..12483)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(12018..12037)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12559..12578
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcagg
1321 aggaattcac catggcatat gagtgaagtt gaattcagcc atgaatattg gatgcgtcat
1381 gccctgaccc tggcaaaacg tgcccgcgat gaacgtgaag tgccggttgg tgcggtgctg
1441 gttttaaata atcgtgtgat tggcgaaggc tggaatcgtg cgattggtct gcatgatcct
1501 accgcacatg cagaaatcat ggccctgcgt cagggtggtc tggttatgca aaattatcgc
1561 ctgattgatg ccaccctgta tgtgaccttt gaaccttgtg tgatgtgtgc gggtgcaatg
1621 attcacagtc gtattggccg tgttgtgttt ggcgttcgta atagcaagcg cggtgccgcc
1681 ggcagcctga tgaatgttct gaattatcct ggcatgaatc atcgcgtgga aattaccgag
1741 ggtattctgg ccgatgaatg tgccgcactg ctgtgcgatt tttatcgcat gccgcgtcaa
1801 gtgtttaatg cacaaaagaa agcacagagc agcattaata gcggcagcga gactcccggg
1861 acctcagagt ccgccacacc cgaaagtaac acgattaaca tcgctaagaa cgacttctct
1921 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta
1981 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
2041 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
2101 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
2161 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
2221 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca
2281 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
2341 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag
2401 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
2461 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta
2521 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
2581 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
2641 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta
2701 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
2761 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac
2821 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
2881 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
2941 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag
3001 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
3061 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
3121 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
3181 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
3241 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
3301 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
3361 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
3421 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
3481 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat
3541 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
3601 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
3661 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
3721 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
3781 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
3841 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
3901 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
3961 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
4021 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
4081 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
4141 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
4201 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
4261 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
4321 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
4381 gttgacacct atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
4441 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
4501 cgtgacatct tagagtcgga cttcgcgttc gcgtaactgc agtataagct tggctgtttt
4561 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
4621 ataaaacaga atttgcctgg cggcagactt ttcatactcc cgccattcag agaagaaacc
4681 aattgtccat attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta
4741 accaaaccgg taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg
4801 acaaaaacgc gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg
4861 cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc ggatcctacc
4921 tgacgctttt tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagcgaa
4981 ttcgagctca ggaggaattc accatggcat tatcatatga tgacagacgc cgagtacgtg
5041 cgcattcatg agaaactgga tatttacacc ttcaagaagc agttcttcaa caacaagaaa
5101 tctgtgtcac accgctgcta cgtgctgttt gagttgaagc gaaggggcga aagaagggct
5161 tgcttttggg gctatgccgt caacaagccc caaagtggca ccgagagagg aatacacgct
5221 gagatattca gtatccgaaa ggtggaagag tatcttcggg ataatcctgg gcagtttacg
5281 atcaactggt attccagctg gagtccttgc gctgattgtg ccgagaaaat tctggaatgg
5341 tataatcagg aacttcgggg aaacgggcac acattgaaaa tctgggcctg caagctgtac
5401 tacgagaaga atgcccggaa ccagatagga ctctggaatc tgagggacaa tggtgtaggc
5461 ctgaacgtga tggtttccga gcactatcag tgttgtcgga agattttcat ccaaagctct
5521 cataaccagc tcaatgaaaa ccgctggttg gagaaaacac tgaaacgtgc ggagaagtgg
5581 agatccgagc tgagcatcat gatccaggtc aagattctgc ataccactaa gtctccagcc
5641 gttagcgaga ctcccgggac ctcagagtcc gccacacccg aaagtaacac gattaacatc
5701 gctaagaacg acttctctga catcgaactg gctgctatcc cgttcaacac tctggctgac
5761 cattacggtg agcgtttagc tcgcgaacag ttggcccttg agcatgagtc ttacgagatg
5821 ggtgaagcac gcttccgcaa gatgtttgag cgtcaactta aagctggtga ggttgcggat
5881 aacgctgccg ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac
5941 gactggtttg aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg
6001 caagaaatca agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta
6061 accagtgctg acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag
6121 gacgaggctc gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt
6181 gaggaacaac tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc
6241 gaggctgaca tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag
6301 gaagactcta ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg
6361 gttagcttac accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc
6421 gcacctgaat acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctccg
6481 atgttccaac cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat
6541 tgggctaacg gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg
6601 cgctacgaag acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc
6661 gcatggaaaa tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat
6721 tgtccggtcg aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac
6781 atcgacatga atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc
6841 aaggacaagg ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat
6901 aagtttgcta accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt
6961 tacgctgtgt caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg
7021 gcgaaaggta aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac
7081 tgtgcgggtg tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac
7141 gagaacatca tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat
7201 tctccgttct gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg
7261 agctataact gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc
7321 tccgcgatgc tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc
7381 gttcaggaca tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca
7441 atcaatggga ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct
7501 gagaaagtca agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact
7561 cgcagtgtga ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc
7621 cgtcaacaag tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg
7681 ttcactcagc cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc
7741 gtgacggtgg tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg
7801 gctgctgagg tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat
7861 tgggtaactc ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc
7921 ttgaacctga tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat
7981 agcgagattg atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa
8041 gacggtagcc accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct
8101 tttgcactga ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa
8161 gcagtgcgcg aaactatggt tgacacctat gagtcttgtg atgtactggc tgatttctac
8221 gaccagttcg ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct
8281 aaaggtaact tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaactgcag
8341 cccggggatc ctaacagagc gcgctctgtt tgagtactag caataaataa ggaggatttt
8401 ttatgaccaa cctttccgac atcatagaga aggaaacagg caaacagttg gtcatccaag
8461 agtcgatact catgcttcct gaagaagttg aggaggtcat tgggaataag ccggaaagtg
8521 acattctcgt acacactgcg tatgatgaga gcaccgatga gaacgtgatg ctgctcacgt
8581 cagatgcccc agagtacaaa ccctgggctc tggtgattca ggactctaat ggagagaaca
8641 agatcaagat gctgtaaaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
8701 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
8761 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
8821 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
8881 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
8941 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
9001 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
9061 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
9121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
9181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
9241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
9301 agcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
9361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
9421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
9481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
9541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
9601 tcagaccaag tttactcata tatactttag attgatttac gcgccctgta gcggcgcatt
9661 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
9721 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
9781 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
9841 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
9901 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
9961 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc
10021 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
10081 aacgtttaca atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
10141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
10201 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
10261 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
10321 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
10381 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtca
10441 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
10501 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
10561 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
10621 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
10681 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
10741 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
10801 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
10861 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
10921 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
10981 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
11041 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
11101 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
11161 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
11221 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
11281 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
11341 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
11401 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
11461 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
11521 caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
11581 tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt
11641 gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat
11701 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
11761 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
11821 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
11881 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
11941 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
12001 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
12061 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
12121 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
12181 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
12241 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
12301 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
12361 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
12421 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
12481 caagaagatc atcttattaa tcagataaaa tatttctagg ctcatgagcc cgaagtggcg
12541 agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
12601 gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt tgacagctta
12661 tc
//
