| 质粒名称: | pDD162 (Peft-3::Cas9 + Empty sgRNA) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 47549 |
| 存储实验室: | Bob Goldstein |
|
载体骨架基本信息 |
|
| 载体名称: | pCFJ601 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2300 bp |
| 完整质粒大小: | 8113 bp |
| 载体修饰: | 由pCFJ601产生，用Cas9替换Mos1转座酶，然后插入U6启动子：：sgRNA基因。克隆方法为Gibson组装（选择“结扎独立克隆”作为通用描述）。 |
| 载体类型: | 蠕虫表达，CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | 合成 |
| 插入大小: | 4323 bp |
| 基因突变: | 线虫密码子优化及内含子合成 |
| 启动子 | eef-1A.1 (eft-3) |
| 标签/融合蛋白: |
NLS（插入的C端子）
HA（插入的C端子）
|
| 克隆方法: | 非连接克隆 |
| 5' 测序引物 | TCAGTTGGGAAACACTTTGCT |
| 3' 测序引物 | gcttgaaaggattttgcatttatc |
|
文献引用方法 |
|
| 材料和方法部分: | pDD162 (Peft-3::Cas9 + Empty sgRNA) was a gift from Bob Goldstein (Addgene plasmid # 47549 ; http://n2t.net/addgene:47549 ; RRID:Addgene_47549) |
| 参考文献部分: | Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination. Dickinson DJ, Ward JD, Reiner DJ, Goldstein B. Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. 10.1038/nmeth.2641 PubMed 23995389 |
买家导航
pDD162 (Peft-3::Cas9 + Empty sgRNA)载体质粒基本信息
pDD162 (Peft-3::Cas9 + Empty sgRNA)质粒图谱载体图谱和pDD162 (Peft-3::Cas9 + Empty sgRNA)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pDD162 (Peft-3::Cas9 + Empty sgRNA)质粒载体简介
pDD162 (Peft-3::Cas9 + Empty sgRNA)质粒序列载体序列
LOCUS pDD162 (Peft-3::Cas9 + Empty sgRNA) 8113 bp ds-DNA circular SYN 13-NOV-2018
DEFINITION Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target
site in the C. elegans genome..
ACCESSION .
VERSION .
KEYWORDS pDD162 (Peft-3::Cas9 + Empty sgRNA)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8113)
AUTHORS Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
TITLE Engineering the Caenorhabditis elegans genome using Cas9-triggered
homologous recombination.
JOURNAL Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
PUBMED 23995389
REFERENCE 2 (bases 1 to 8113)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 13, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8113
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1..589
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 490..509
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 716..738
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 735..751
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 735..751
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
protein_bind 771..791
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 792..1391
/gene="eft-3"
/label=eft-3 promoter
/note="promoter for the C. elegans elongation factor
1-alpha gene (Mitrovich and Anderson, 2000)"
CDS join(1408..2451,2503..3573,3625..4650,4702..5664)
/codon_start=1
/product="S. pyogenes Cas9, codon optimized for C. elegans
with synthetic introns"
/label=Cas9
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5680..5700
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5701..5727
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 5742..6065
/gene="tbb-2"
/label=tbb-2 terminator
/note="transcription terminator for the C. elegans tubulin
beta-2 gene"
protein_bind complement(6069..6089)
/gene="mutant version of attB"
/label=attB3
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
primer_bind complement(6097..6114)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6097..6113)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6106..6128)
/label=M13/pUC Forward
/note="In lacZ gene"
promoter 6134..6483
/label=CeU6 promoter
/note="promoter for the C. elegans R07E5.16 U6 snRNA gene
(Dickinson et al., 2013)"
misc_RNA 6485..6560
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 6832..6854
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6892..6910)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6978..7082
/gene="bla"
/label=AmpR promoter
CDS 7083..7943
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7301..7320)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
601 cgcggccttt ttacggttcc tggtcttttg ctggcctttt gctcacatgt tctttcctgc
661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccagcgg
721 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat caactttgta
781 tagaaaagtt ggcacctttg gtcttttatt gtcaacttcc attggttctt ccattgtttc
841 tgttaaatta atgaattttt cataaaataa agacattata caatataaaa atgaagaatt
901 tattgaaaat aaactgccag agagaaaaag tatgcaacac tcccgccgag agtgtttgaa
961 atggtgtacg gtacattttc gtgctaggag ttagatgtgc aggcagcaac gagaggggga
1021 gagatttttt tgggccttgt gaaattaacg tgagttttct ggtcatctga ctaatcatgt
1081 tggttttttg ttggtttatt ttgtttttat ctttgttttt atccagatta ggaaatttaa
1141 attttatgaa tttataatga ggtcaaacat tcagtcccag cgtttttcct gttctcactg
1201 tttagtcgaa tttttatttt aggctttcaa caaatgttct aactgtctta tttgtgacct
1261 cactttttat atttttttaa tttttaaaaa tattagaagt ttctaggata attttttcga
1321 cttttattct ctctaccgtc cgcactcttc ttacttttaa attaaattgt ttttttttca
1381 gttgggaaac actttgctct agaaaaaatg gacaaaaaat acagcatcgg ccttgacatc
1441 ggaacgaact ccgtcggatg ggccgtcatc accgacgagt acaaggtccc atccaagaag
1501 ttcaaggtcc tcggaaacac cgaccgtcac tccatcaaga agaacctcat cggagccctc
1561 ctcttcgact ccggagagac cgccgaggcc acccgtctca agcgtaccgc ccgtcgtcgt
1621 tacacccgtc gtaagaaccg tatctgctac ctccaagaga tcttctccaa cgagatggcc
1681 aaggtcgacg actccttctt ccaccgtctc gaggagtcct tcctcgtcga ggaggacaag
1741 aagcacgagc gtcacccaat cttcggaaac atcgtcgacg aggtcgccta ccacgagaag
1801 tacccaacca tctaccacct ccgtaagaag ctcgtcgact ccaccgacaa ggccgacctc
1861 cgtctcatct acctcgccct cgcccacatg atcaagttcc gtggacactt cctcatcgag
1921 ggagacctca acccagacaa ctccgacgtc gacaagctct tcatccaact cgtccaaacc
1981 tacaaccaac tcttcgagga gaacccaatc aacgcctccg gagtcgacgc caaggccatc
2041 ctctccgccc gtctctccaa gtcccgtcgt ctcgagaacc tcatcgccca actcccagga
2101 gagaagaaga acggactctt cggaaacctc atcgccctct ccctcggact caccccaaac
2161 ttcaagtcca acttcgacct cgccgaggac gccaagctcc aactctccaa ggacacctac
2221 gacgacgacc tcgacaacct cctcgcccaa atcggagacc aatacgccga cctcttcctc
2281 gccgccaaga acctctccga cgccatcctc ctctccgaca tcctccgtgt caacaccgag
2341 atcaccaagg ccccactctc cgcctccatg atcaagcgtt acgacgagca ccaccaagac
2401 ctcaccctcc tcaaggccct cgtccgtcaa caactcccag agaagtacaa ggtaagttta
2461 aacatatata tactaactaa ccctgattat ttaaattttc aggagatctt cttcgaccaa
2521 tccaagaacg gatacgccgg atacatcgac ggaggagcct cccaagagga gttctacaag
2581 ttcatcaagc caatcctcga gaagatggac ggaaccgagg agctcctcgt caagctcaac
2641 cgtgaggacc tcctccgtaa gcaacgtacc ttcgacaacg gatccatccc acaccaaatc
2701 cacctcggag agctccacgc catcctccgt cgtcaagagg acttctaccc attcctcaag
2761 gacaaccgtg agaagatcga gaagatcctc accttccgta tcccatacta cgtcggacca
2821 ctcgcccgtg gaaactcccg tttcgcctgg atgacccgta agtccgagga gaccatcacc
2881 ccatggaact tcgaggaggt cgtcgacaag ggagcctccg cccaatcctt catcgagcgt
2941 atgaccaact tcgacaagaa cctcccaaac gagaaggtcc tcccaaagca ctccctcctc
3001 tacgagtact tcaccgtcta caacgagctc accaaggtca agtacgtcac cgagggaatg
3061 cgtaagccag ccttcctctc cggagagcaa aagaaggcca tcgtcgacct cctcttcaag
3121 accaaccgta aggtcaccgt caagcaactc aaggaggact acttcaagaa gatcgagtgc
3181 ttcgactccg tcgagatctc cggagtcgag gaccgtttca acgcctccct cggaacctac
3241 cacgacctcc tcaagatcat caaggacaag gacttcctcg acaacgagga gaacgaggac
3301 atcctcgagg acatcgtcct caccctcacc ctcttcgagg accgtgagat gatcgaggag
3361 cgtctcaaga cctacgccca cctcttcgac gacaaggtca tgaagcagct taagcgtcgt
3421 cgttacaccg gatggggacg tctctcccgt aagctcatca acggaatccg tgacaagcaa
3481 tccggaaaga ccatcctcga cttcctcaag tccgacggat tcgccaaccg taacttcatg
3541 caactcatcc acgacgactc cctcaccttc aaggtaagtt taaacagttc ggtactaact
3601 aaccatacat atttaaattt tcaggaggac atccaaaagg cccaagtctc cggacaagga
3661 gactccctcc acgagcacat cgccaacctc gccggatccc cagccatcaa gaagggaatc
3721 ctccaaaccg tcaaggtcgt cgacgagctc gtcaaggtca tgggacgtca caagccagag
3781 aacatcgtca tcgagatggc ccgtgagaac caaaccaccc aaaagggaca aaagaactcc
3841 cgtgagcgta tgaagcgtat cgaggaggga atcaaggagc tcggatccca aatcctcaag
3901 gagcacccag tcgagaacac ccaactccaa aacgagaagc tctacctcta ctacctccaa
3961 aacggacgtg acatgtacgt cgaccaagag ctcgacatca accgtctctc cgactacgac
4021 gtcgaccaca tcgtcccaca atccttcctc aaggacgact ccatcgacaa caaggtcctc
4081 acccgttccg acaagaaccg tggaaagtcc gacaacgtcc catccgagga ggtcgtcaag
4141 aagatgaaga actactggcg tcaactcctc aacgccaagc tcatcaccca acgtaagttc
4201 gacaacctca ccaaggctga gcgtggagga ctctccgagc tcgacaaggc cggattcatc
4261 aagcgtcaac tcgtcgagac ccgtcaaatc accaagcacg tcgcccaaat cctcgactcc
4321 cgtatgaaca ccaagtacga cgagaacgac aagctcatcc gtgaggtcaa ggtcatcacc
4381 ctcaagtcca agctcgtctc cgacttccgt aaggacttcc aattctacaa ggtccgtgag
4441 atcaacaact accaccacgc ccacgacgcc tacctcaacg ccgtagtagg aaccgccctc
4501 atcaagaagt acccaaagct cgagtccgag ttcgtctacg gagactacaa ggtctacgac
4561 gtccgtaaga tgatcgccaa gtccgagcaa gagatcggaa aggccaccgc caagtacttc
4621 ttctactcca acatcatgaa cttcttcaag gtaagtttaa acatgatttt actaactaac
4681 taatctgatt taaattttca gaccgagatc accctcgcca acggagagat ccgtaagcgt
4741 ccactcatcg agaccaacgg agagaccgga gagatcgtct gggacaaggg acgtgacttc
4801 gccacagtcc gtaaggtcct ctccatgcca caagtcaaca tcgtcaagaa gaccgaggtc
4861 caaaccggag gattctccaa ggagtccatc ctcccaaagc gtaactccga caagctcatc
4921 gcccgtaaga aggactggga cccaaagaag tacggaggat tcgactcccc aaccgtcgcc
4981 tactccgtcc tcgtcgtcgc caaggtcgag aagggaaagt ccaagaagct caagtccgtc
5041 aaggagctcc tcggaatcac catcatggag cgttcctcct tcgagaagaa cccaatcgac
5101 ttcctcgagg ccaagggata caaggaggtc aagaaggacc tcatcatcaa gctcccaaag
5161 tactccctct tcgagctcga gaacggacgt aagcgtatgc tcgcctccgc cggagagctc
5221 caaaagggaa acgagctcgc cctcccatcc aagtacgtca acttcctcta cctcgcctcc
5281 cactacgaga agctcaaggg atccccagag gacaacgagc aaaagcaact cttcgtcgag
5341 caacacaagc actacctcga cgagatcatc gagcaaatct ccgagttctc caagcgtgtc
5401 atcctcgccg acgccaacct cgacaaggtc ctctccgcct acaacaagca ccgtgacaag
5461 ccaatccgtg agcaagccga gaacatcatc cacctcttca ccctcaccaa cctcggagcc
5521 ccagccgcct tcaagtactt cgacaccacc atcgaccgta agcgttacac ctccaccaag
5581 gaggtcctcg acgccaccct catccaccaa tccatcaccg gactctacga gacccgtatc
5641 gacctctccc aactcggagg agacggagga tccggaccac caaagaagaa gcgtaaggtc
5701 tacccatacg acgtcccaga ctacgcctaa actagtgata aatgcaaaat cctttcaagc
5761 attcccttct tctctatcac tcttctttct ttttgtcaaa aaattctctc gctaatttat
5821 ttgctttttt aatgttatta ttttatgact ttttatagtc actgaaaagt ttgcatctga
5881 gtgaagtgaa tgctatcaaa atgtgattct gtctgatgta ctttcacaat ctctcttcaa
5941 ttccattttg aagtgcttta aacccgaaag gttgagaaaa atgcgagcgc tcaaatattt
6001 gtattgtgtt cgttgagtga cccaacaaaa agaggaaact ttattgtgcc gccaagaaaa
6061 aagtctcaca actttattat acatagttga taattcactg gccgtcgttt tacaacgtcg
6121 tgactgggaa aaccaaaaaa aactagcaat aaaggaataa aaaactgtac accttaaagg
6181 cgcacactct gttttgcaaa ttttattttt agttgtgaat tttctgctga gacctgaaaa
6241 tagcaacttt agtactacta taatttgtca accttttcaa aaaaagcatg caatttttga
6301 gaaactctta taaaagctat tattaaaaaa acaccttttt tccaaaatta ttccacaaaa
6361 aatatgttat gaaatgccta caccctctca cacacactct ttatactact ctgtcaaact
6421 cacgagatgt ctgccgcctc ttgtgttgcc cctatataaa cacctcctat tgcgagatgt
6481 cttggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa
6541 aagtggcacc gagtcggtgc tttttgtgaa atttctggcg taatagcgaa gaggcccgca
6601 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
6661 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtgcaatct
6721 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
6781 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
6841 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
6901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
6961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
7021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
7081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
7141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
7201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
7261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
7321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
7381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
7441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
7501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
7561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
7621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
7681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
7741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
7801 ctcgcggtat cattgcagca ctggggtcag atggtaagcc ctcccgtatc gtagttatct
7861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
7921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
7981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
8041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
8101 tcaaaggatc ttc
//
pDD162 (Peft-3::Cas9 + Empty sgRNA)载体图谱质粒图谱pdf版和pDD162 (Peft-3::Cas9 + Empty sgRNA)载体序列质粒序列等相关资料下载
pDD162 (Peft-3::Cas9 + Empty sgRNA)质粒载体应用举例
- 上一篇：pCRISPR
- 下一篇：pET28a-sfGFP
