| 质粒名称: | pCW57-MCS1-P2A-MCS2 (RFP) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80923 |
| 存储实验室: | Adam Karpf |
|
载体骨架基本信息 |
|
| 载体名称: | pCW57.1 |
| 载体出品公司: | Broad Insitute |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 7834 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 用turbo-RFP代替嘌呤霉素对pCW57-MCS1-2A-MCS2进行了改性。 |
| 载体类型: | 哺乳动物表达，慢病毒；强力霉素诱导；P2A自切割肽；Turbo RFP reporter |
| 筛选标记: | RFP |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | TRE |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pCW57.MCS_Seq_F CGTATGTCGAGGTAGGCGTG |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCW57-MCS1-P2A-MCS2 (RFP) was a gift from Adam Karpf (Addgene plasmid # 80923 ; http://n2t.net/addgene:80923 ; RRID:Addgene_80923) |
| 参考文献部分: | Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression in Cancer. Barger CJ, Branick C, Chee L, Karpf AR. Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 10.3390/cancers11020251. 10.3390/cancers11020251 PubMed 30795624 |
买家导航
pCW57-MCS1-P2A-MCS2 (RFP)载体质粒基本信息
pCW57-MCS1-P2A-MCS2 (RFP)质粒图谱载体图谱和pCW57-MCS1-P2A-MCS2 (RFP)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pCW57-MCS1-P2A-MCS2 (RFP)质粒载体简介
pCW57-MCS1-P2A-MCS2 (RFP)质粒序列载体序列
LOCUS pCW57-MCS1-P2A-MCS2 (RFP) 7834 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION All-in-one doxycycline inducible lentiviral vector for expression of
one or two genes using the P2A self-cleaving peptide..
ACCESSION .
VERSION .
KEYWORDS pCW57-MCS1-P2A-MCS2 (RFP)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7834)
TITLE All-in-one doxycycline inducible lentiviral vector for expression of
one or two genes.
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7834)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7834
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 41..158
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 212..526
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 220..238
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 256..274
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 291..309
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 327..345
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 363..381
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 398..416
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 434..452
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 499..523
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 567..623
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
promoter 651..1161
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1080..1098
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1185..1877
/codon_start=1
/product="red fluorescent protein from Entacmaea
quadricolor"
/label=TurboRFP
/note="mammalian codon-optimized"
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
CDS 1893..1946
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 1947..2693
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/label=rtTA-Advanced
/note="In the Tet-On(R) system, rtTA-Advanced binds to the
Tet-responsive element and stimulates transcription only in
the presence of tetracycline or doxycycline."
/translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
primer_bind complement(2733..2753)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3151..3162)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3367..3600
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3672..3793
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3709..3728)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3763..3782
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3833..3968
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3895..3914
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 3968..4039
/gene="bla"
/label=AmpR promoter
CDS 4040..4900
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4258..4277)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5071..5659
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5560..5579
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5813..5830
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5947..5968
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5983..6013
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6021..6037
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6026..6048
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6045..6061
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6045..6061
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6080..6100
/label=T3
/note="T3 promoter, forward primer"
promoter 6082..6100
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6128..6354
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6355..6535
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6582..6707
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7200..7433
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
541 gcgaattcgt cgacaccggt tccggagcca cgaacttctc tctgttaaag caagcaggag
601 acgtggaaga aaaccccggt cctaggctgc agacgcgttg tacaggatcc ggggttgggg
661 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
721 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
781 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
841 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
901 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
961 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
1021 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
1081 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
1141 atcaccgacc tctctcccca gcaattcacc cgcgcgcggc caccatgagc gagctgatca
1201 aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
1261 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag atcaaggtgg
1321 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
1381 gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg
1441 agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg accgctaccc
1501 aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac ggggtgaact
1561 tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc aacaccgaga
1621 tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg aagctcgtgg
1681 gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa cccgctaaga
1741 acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc aaggaggccg
1801 acaaagagac ctacgtcgag cagcacgaga tggctgtggc caagtactgc gacctcccta
1861 gcaaactggg gcacagattc gaaggaagcg gagaaggtag aggttctctc ctcacttgtg
1921 gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg
1981 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
2041 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
2101 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
2161 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
2221 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
2281 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
2341 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
2401 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
2461 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
2521 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
2581 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
2641 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
2701 aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac
2761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
2821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
2881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
2941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
3001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
3061 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
3121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
3181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
3241 cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac
3301 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
3361 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
3421 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
3481 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
3541 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
3601 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
3661 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
3721 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
3781 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
3841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
3901 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
3961 tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4021 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
4081 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4141 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4201 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4261 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4321 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4381 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4441 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4501 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
4561 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
4621 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
4681 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
4741 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
4801 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
4861 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
4921 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
4981 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5041 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5101 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5161 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5221 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5281 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5341 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5401 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5461 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
5521 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
5581 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
5641 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
5701 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
5761 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
5821 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
5881 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
5941 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
6001 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
6061 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
6121 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
6181 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
6241 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
6301 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
6361 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
6421 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
6481 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
6541 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
6601 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
6661 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
6721 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
6781 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
6841 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
6901 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
6961 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
7021 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
7081 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
7141 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
7201 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
7261 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
7321 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
7381 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
7441 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
7501 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
7561 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
7621 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
7681 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
7741 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
7801 tcgtttcaga cccacctccc aaccccgagg ggac
//
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