| 质粒名称: | pCAS9-mCherry empty |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80975 |
| 存储实验室: | Veit Hornung |
|
载体骨架基本信息 |
|
| 载体名称: | pCAS9 |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | None |
|
文献引用方法 |
|
| 材料和方法部分: | pCAS9-mCherry empty was a gift from Veit Hornung (爱迪基因 plasmid # 80975) |
| 参考文献部分: | CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism. Schmid-Burgk JL, Honing K, Ebert TS, Hornung V. Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338. 10.1038/ncomms12338 PubMed 27465542 |
买家导航
pCAS9-mCherry empty载体质粒基本信息
pCAS9-mCherry empty质粒图谱载体图谱和pCAS9-mCherry empty载体序列质粒序列多克隆位点信息
pCAS9-mCherry empty质粒载体简介
pCAS9-mCherry empty质粒序列载体序列
LOCUS pCAS9-mCherry empty 10048 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION Cas9, mCherry, and sgRNA expression.
ACCESSION .
VERSION .
KEYWORDS pCAS9-mCherry empty
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10048)
AUTHORS Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
TITLE CRISPaint allows modular base-specific gene tagging using a
ligase-4-dependent mechanism.
JOURNAL Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
PUBMED 27465542
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCAS9-mCherry empty|
FEATURES Location/Qualifiers
source 1..10048
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS 938..1354
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1322..1338
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
promoter 1365..1605
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1365..1385
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 1536..1555
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
enhancer 1712..2091
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2092..2295
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2246..2266
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 2350..3057
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2495..2513)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2763..2782
/label=mCherry-F
/note="mCherry, forward primer"
CDS 3082..3135
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3136..7239
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7252..7272
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
LTR 7329..7922
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(8123..8140)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8294..8882)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8374..8393)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9060..9920)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9683..9702
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9921..10025)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ctagttagct aactagctct gtatctggcg gacccgtggt
661 ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag ggactttggg
721 ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga
781 tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg
841 ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
901 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
961 aagtttgacc ttagatcact ggaaagatgt cgagcggctc gctcacaacc agtcggtaga
1021 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
1081 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
1141 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
1201 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
1261 tcttccatcc gcgccgtctc tcccccttga acctcctctt tcgaccccgc ctcaatcctc
1321 cctttatcca gccctcactc cttctctagg cgccggccgg atccgagggc ctatttccca
1381 tgattccttc atatttgcat atacgataca aggctgttag agagataatt ggaattaatt
1441 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
1501 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
1561 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggccct
1621 cgatatcgac tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttcg
1681 aattccgatg tacgggccag atatacgcgt tgacattgat tattgtctag ttattaatag
1741 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
1801 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
1861 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
1921 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
1981 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
2041 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
2101 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
2161 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
2221 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
2281 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
2341 aagcttccca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
2401 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
2461 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
2521 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
2581 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
2641 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
2701 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
2761 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
2821 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
2881 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
2941 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
3001 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggtc
3061 gctaccatcg agggatccgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag
3121 gagaatcctg gcccaatgga caagaagtac agcatcggcc tggacatcgg caccaacagc
3181 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaagtt caaggtgctg
3241 ggcaacaccg accgccacag catcaagaag aacctgatcg gcgccctgct gttcgacagc
3301 ggcgagaccg ccgaggccac ccgcctgaag cgcaccgccc gccgccgcta cacccgccgc
3361 aagaaccgca tctgctacct gcaggagatc ttcagcaacg agatggccaa ggtggacgac
3421 agcttcttcc accgcctgga ggagagcttc ctggtggagg aggacaagaa gcacgagcgc
3481 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
3541 taccacctgc gcaagaagct ggtggacagc accgacaagg ccgacctgcg cctgatctac
3601 ctggccctgg cccacatgat caagttccgc ggccacttcc tgatcgaggg cgacctgaac
3661 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
3721 ttcgaggaga accccatcaa cgccagcggc gtggacgcca aggccatcct gagcgcccgc
3781 ctgagcaaga gccgccgcct ggagaacctg atcgcccagc tgcccggcga gaagaagaac
3841 ggcctgttcg gcaacctgat cgccctgagc ctgggcctga cccccaactt caagagcaac
3901 ttcgacctgg ccgaggacgc caagctgcag ctgagcaagg acacctacga cgacgacctg
3961 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgttcctggc cgccaagaac
4021 ctgagcgacg ccatcctgct gagcgacatc ctgcgcgtga acaccgagat caccaaggcc
4081 cccctgagcg ccagcatgat caagcgctac gacgagcacc accaggacct gaccctgctg
4141 aaggccctgg tgcgccagca gctgcccgag aagtacaagg agatcttctt cgaccagagc
4201 aagaacggct acgccggcta catcgacggc ggcgccagcc aggaggagtt ctacaagttc
4261 atcaagccca tcctggagaa gatggacggc accgaggagc tgctggtgaa gctgaaccgc
4321 gaggacctgc tgcgcaagca gcgcaccttc gacaacggca gcatccccca ccagatccac
4381 ctgggcgagc tgcacgccat cctgcgccgc caggaggact tctacccctt cctgaaggac
4441 aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc cctactacgt cggccccctg
4501 gcccgcggca acagccgctt cgcctggatg acccgcaaga gcgaggagac catcaccccc
4561 tggaacttcg aggaggtggt ggacaagggc gccagcgccc agagcttcat cgagcgcatg
4621 accaacttcg acaagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
4681 gagtacttca ccgtgtacaa cgagctgacc aaggtgaagt acgtgaccga gggcatgcgc
4741 aagcccgcct tcctgagcgg cgagcagaag aaggccatcg tggacctgct gttcaagacc
4801 aaccgcaagg tgaccgtgaa gcagctgaag gaggactact tcaagaagat cgagtgcttc
4861 gacagcgtgg agatcagcgg cgtggaggac cgcttcaacg ccagcctggg cacctaccac
4921 gacctgctga agatcatcaa ggacaaggac ttcctggaca acgaggagaa cgaggacatc
4981 ctggaggaca tcgtgctgac cctgaccctg ttcgaggacc gcgagatgat cgaggagcgc
5041 ctgaagacct acgcccacct gttcgacgac aaggtgatga agcagctgaa gcgccgccgc
5101 tacaccggct ggggccgcct gagccgcaag ctgatcaacg gcatccgcga caagcagagc
5161 ggcaagacca tcctggactt cctgaagagc gacggcttcg ccaaccgcaa cttcatgcag
5221 ctgatccacg acgacagcct gaccttcaag gaggacatcc agaaggccca ggtgagcggc
5281 cagggcgaca gcctgcacga gcacatcgcc aacctggccg gcagccccgc catcaagaag
5341 ggcatcctgc agaccgtgaa ggtggtggac gagctggtga aggtgatggg ccgccacaag
5401 cccgagaaca tcgtgatcga gatggcccgc gagaaccaga ccacccagaa gggccagaag
5461 aacagccgcg agcgcatgaa gcgcatcgag gagggcatca aggagctggg cagccagatc
5521 ctgaaggagc accccgtgga gaacacccag ctgcagaacg agaagctgta cctgtactac
5581 ctgcagaacg gccgcgacat gtacgtggac caggagctgg acatcaaccg cctgagcgac
5641 tacgacgtgg accacatcgt gccccagagc ttcctgaagg acgacagcat cgacaacaag
5701 gtgctgaccc gcagcgacaa gaaccgcggc aagagcgaca acgtgcccag cgaggaggtg
5761 gtgaagaaga tgaagaacta ctggcgccag ctgctgaacg ccaagctgat cacccagcgc
5821 aagttcgaca acctgaccaa ggccgagcgc ggcggcctga gcgagctgga caaggccggc
5881 ttcatcaagc gccagctggt ggagacccgc cagatcacca agcacgtggc ccagatcctg
5941 gacagccgca tgaacaccaa gtacgacgag aacgacaagc tgatccgcga ggtgaaggtg
6001 atcaccctga agagcaagct ggtgagcgac ttccgcaagg acttccagtt ctacaaggtg
6061 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt ggtgggcacc
6121 gccctgatca agaagtaccc caagctggag agcgagttcg tgtacggcga ctacaaggtg
6181 tacgacgtgc gcaagatgat cgccaagagc gagcaggaga tcggcaaggc caccgccaag
6241 tacttcttct acagcaacat catgaacttc ttcaagaccg agatcaccct ggccaacggc
6301 gagatccgca agcgccccct gatcgagacc aacggcgaga ccggcgagat cgtgtgggac
6361 aagggccgcg acttcgccac cgtgcgcaag gtgctgagca tgccccaggt gaacatcgtg
6421 aagaagaccg aggtgcagac cggcggcttc agcaaggaga gcatcctgcc caagcgcaac
6481 agcgacaagc tgatcgcccg caagaaggac tgggacccca agaagtacgg cggcttcgac
6541 agccccaccg tggcctacag cgtgctggtg gtggccaagg tggagaaggg caagagcaag
6601 aagctgaaga gcgtgaagga gctgctgggc atcaccatca tggagcgcag cagcttcgag
6661 aagaacccca tcgacttcct ggaggccaag ggctacaagg aggtgaagaa ggacctgatc
6721 atcaagctgc ccaagtacag cctgttcgag ctggagaacg gccgcaagcg catgctggcc
6781 agcgccggcg agctgcagaa gggcaacgag ctggccctgc ccagcaagta cgtgaacttc
6841 ctgtacctgg ccagccacta cgagaagctg aagggcagcc ccgaggacaa cgagcagaag
6901 cagctgttcg tggagcagca caagcactac ctggacgaga tcatcgagca gatcagcgag
6961 ttcagcaagc gcgtgatcct ggccgacgcc aacctggaca aggtgctgag cgcctacaac
7021 aagcaccgcg acaagcccat ccgcgagcag gccgagaaca tcatccacct gttcaccctg
7081 accaacctgg gcgcccccgc cgccttcaag tacttcgaca ccaccatcga ccgcaagcgc
7141 tacaccagca ccaaggaggt gctggacgcc accctgatcc accagagcat caccggcctg
7201 tacgagaccc gcatcgacct gagccagctg ggcggcgaca gccgcgccga ccccaagaag
7261 aagcgcaagg tgtgagcggc cgcatcgata aaataaaaga ttttatttag tctccagaaa
7321 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
7381 tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca
7441 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
7501 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
7561 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
7621 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
7681 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
7741 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
7801 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
7861 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
7921 tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg actacccgtc
7981 agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
8041 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
8101 cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
8161 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
8221 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
8281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
8341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
8401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
8461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
8521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
8581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
8641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
8701 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
8761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
8821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
8881 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
8941 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
9001 aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
9061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
9121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
9181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
9241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
9301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
9361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
9421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
9481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
9541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
9601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
9661 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
9721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
9781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
9841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
9901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
9961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
10021 ccgcgcacat ttccccgaaa agtgccac
//
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